LOCUS       sequence-168649-        6558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2,part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2_p.T182I
FEATURES             Location/Qualifiers
     source          1..6558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      71..659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     560..579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     813..830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(989..1016)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1108..1194)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1243..1265
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1257..1274
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1258..1274
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1290..1389
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5158..5257)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5274..5293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5275..5293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5298..5314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5298..5314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5745..6536
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
       61 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      181 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      661 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      721 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
      781 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      841 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      901 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      961 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     1021 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     1081 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     1141 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     1201 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     1261 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
     1321 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
     1381 aagttggcat ggagctggcg gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc
     1441 ccggagccgc gagcacccaa gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca
     1501 gtcccgagac ccacctggac atgctccgcc acctctacca gggctgccag gtggtgcagg
     1561 gaaacctgga actcacctac ctgcccacca atgccagcct gtccttcctg caggatatcc
     1621 aggaggtgca gggctacgtg ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga
     1681 ggctgcggat tgtgcgaggc acccagctct ttgaggacaa ctatgccctg gccgtgctag
     1741 acaatggaga cccgctgaac aataccaccc ctgtcacagg ggcctcccca ggaggcctgc
     1801 gggagctgca gcttcgaagc ctcacagaga tcttgaaagg aggggtcttg atccagcgga
     1861 acccccagct ctgctaccag gacacgattt tgtggaagga catcttccac aagaacaacc
     1921 agctggctct catactgata gacaccaacc gctctcgggc ctgccacccc tgttctccga
     1981 tgtgtaaggg ctcccgctgc tggggagaga gttctgagga ttgtcagagc ctgacgcgca
     2041 ctgtctgtgc cggtggctgt gcccgctgca aggggccact gcccactgac tgctgccatg
     2101 agcagtgtgc tgccggctgc acgggcccca agcactctga ctgcctggcc tgcctccact
     2161 tcaaccacag tggcatctgt gagctgcact gcccagccct ggtcacctac aacacagaca
     2221 cgtttgagtc catgcccaat cccgagggcc ggtatacatt cggcgccagc tgtgtgactg
     2281 cctgtcccta caactacctt tctacggacg tgggatcctg caccctcgtc tgccccctgc
     2341 acaaccaaga ggtgacagca gaggatggaa cacagcggtg tgagaagtgc agcaagccct
     2401 gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg agaggtgagg gcagttacca
     2461 gtgccaatat ccaggagttt gctggctgca agaagatctt tgggagcctg gcatttctgc
     2521 cggagagctt tgatggggac ccagcctcca acactgcccc gctccagcca gagcagctcc
     2581 aagtgtttga gactctggaa gagatcacag gttacctata catctcagca tggccggaca
     2641 gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat ccggggacga attctgcaca
     2701 atggcgccta ctcgctgacc ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac
     2761 tgagggaact gggcagtgga ctggccctca tccaccataa cacccacctc tgcttcgtgc
     2821 acacggtgcc ctgggaccag ctctttcgga acccgcacca agctctgctc cacactgcca
     2881 accggccaga ggacgagtgt gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag
     2941 ggcactgctg gggtccaggg cccacccagt gtgtcaactg cagccagttc cttcggggcc
     3001 aggagtgcgt ggaggaatgc cgagtactgc aggggctccc cagggagtat gtgaatgcca
     3061 ggcactgttt gccgtgccac cctgagtgtc agccccagaa tggctcagtg acctgttttg
     3121 gaccggaggc tgaccagtgt gtggcctgtg cccactataa ggaccctccc ttctgcgtgg
     3181 cccgctgccc cagcggtgtg aaacctgacc tctcctacat gcccatctgg aagtttccag
     3241 atgaggaggg cgcatgccag ccttgcccca tcaactgcac ccactcctgt gtggacctgg
     3301 atgacaaggg ctgccccgcc gagcagagag ccagccctct gacgtccatc atctctgcgg
     3361 tggttggcat tctgctggtc gtggtcttgg gggtggtctt tgggatcctc atcaagcgac
     3421 ggcagcagaa gatccggaag tacacgatgc ggagactgct gcaggaaacg gagctggtgg
     3481 agccgctgac acctagcgga gcgatgccca accaggcgca gatgcggatc ctgaaagaga
     3541 cggagctgag gaaggtgaag gtgcttggat ctggcgcttt tggcacagtc tacaagggca
     3601 tctggatccc tgatggggag aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa
     3661 acacatcccc caaagccaac aaagaaatct tagacgaagc atacgtgatg gctggtgtgg
     3721 gctccccata tgtctcccgc cttctgggca tctgcctgac atccacggtg cagctggtga
     3781 cacagcttat gccctatggc tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg
     3841 gctcccagga cctgctgaac tggtgtatgc agattgccaa ggggatgagc tacctggagg
     3901 atgtgcggct cgtacacagg gacttggccg ctcggaacgt gctggtcaag agtcccaacc
     3961 atgtcaaaat tacagacttc gggctggctc ggctgctgga cattgacgag acagagtacc
     4021 atgcagatgg gggcaaggtg cccatcaagt ggatggcgct ggagtccatt ctccgccggc
     4081 ggttcaccca ccagagtgat gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt
     4141 ttggggccaa accttacgat gggatcccag cccgggagat ccctgacctg ctggaaaagg
     4201 gggagcggct gccccagccc cccatctgca ccattgatgt ctacatgatc atggtcaaat
     4261 gttggatgat tgactctgaa tgtcggccaa gattccggga gttggtgtct gaattctccc
     4321 gcatggccag ggacccccag cgctttgtgg tcatccagaa tgaggacttg ggcccagcca
     4381 gtcccttgga cagcaccttc taccgctcac tgctggagga cgatgacatg ggggacctgg
     4441 tggatgctga ggagtatctg gtaccccagc agggcttctt ctgtccagac cctgccccgg
     4501 gcgctggggg catggtccac cacaggcacc gcagctcatc taccaggagt ggcggtgggg
     4561 acctgacact agggctggag ccctctgaag aggaggcccc caggtctcca ctggcaccct
     4621 ccgaaggggc tggctccgat gtatttgatg gtgacctggg aatgggggca gccaaggggc
     4681 tgcaaagcct ccccacacat gaccccagcc ctctacagcg gtacagtgag gaccccacag
     4741 tacccctgcc ctctgagact gatggctacg ttgcccccct gacctgcagc ccccagcctg
     4801 aatatgtgaa ccagccagat gttcggcccc agcccccttc gccccgagag ggccctctgc
     4861 ctgctgcccg acctgctggt gccactctgg aaaggcccaa gactctctcc ccagggaaga
     4921 atggggtcgt caaagacgtt tttgcctttg ggggtgccgt ggagaacccc gagtacttga
     4981 caccccaggg aggagctgcc cctcagcccc accctcctcc tgccttcagc ccagccttcg
     5041 acaacctcta ttactgggac caggacccac cagagcgggg ggctccaccc agcaccttca
     5101 aagggacacc tacggcagag aacccagagt acctgggtct ggacgtgcca gtgtgattgc
     5161 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     5221 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     5281 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     5341 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     5401 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     5461 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     5521 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     5581 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     5641 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     5701 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     5761 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     5821 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     5881 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     5941 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     6001 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     6061 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     6121 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     6181 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     6241 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     6301 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     6361 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     6421 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     6481 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     6541 cgagccaccc atgaccaa
//
