LOCUS       sequence-168652-        5478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP4K3, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP4K3_p.Q473H
FEATURES             Location/Qualifiers
     source          1..5478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      38..626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     527..546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     780..797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(956..983)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1075..1161)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1210..1232
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1224..1241
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1225..1241
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1257..1356
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4045..4144)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4161..4180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4162..4180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4185..4201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4185..4201)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4632..5423
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
       61 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
      781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      901 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
      961 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     1021 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     1081 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     1141 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     1201 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa
     1261 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
     1321 tttttataat gccaactttg tacaaaaaag ttggcaccat gaaccccggc ttcgatttgt
     1381 cccgccggaa cccgcaggag gacttcgagc tgattcagcg catcggcagc ggcacctacg
     1441 gcgacgtcta caaggcacgg aatgttaaca ctggtgaatt agcagcaatt aaagtaataa
     1501 aattggaacc aggagaagac tttgcagttg tgcagcaaga aattatcatg atgaaagact
     1561 gtaaacaccc aaatattgtt gcttattttg gaagctatct caggcgagat aagctttgga
     1621 tttgcatgga gttttgtgga ggtggttctt tacaggatat ttatcacgta actggacctc
     1681 tgtcagaact gcaaattgca tatgttagca gagaaacact gcagggatta tattatcttc
     1741 acagtaaagg aaaaatgcac agagatataa agggagctaa cattctatta acggataatg
     1801 gtcatgtgaa attggctgat tttggagtat ctgcacagat aacagctaca attgccaaac
     1861 ggaagtcttt cattggcaca ccatattgga tggctccaga agttgcagct gttgagagga
     1921 aggggggtta caatcaactc tgtgatctct gggcagtggg aatcactgcc atagaacttg
     1981 cagagcttca gcctcctatg tttgacttac acccaatgag agcattattt ctaatgacaa
     2041 aaagcaattt tcagcctcct aaactaaagg ataaaatgaa atggtcaaat agttttcatc
     2101 actttgtgaa aatggcactt accaaaaatc cgaaaaaaag acctactgct gaaaaattat
     2161 tacagcatcc ttttgtaaca caacatttga cacggtcttt ggcaatcgag ctgttggata
     2221 aagtaaataa tccagatcat tccacttacc atgatttcga tgatgatgat cctgagcctc
     2281 ttgttgctgt accacataga attcactcaa caagtagaaa cgtgagagaa gaaaaaacac
     2341 gctcagagat aacctttggc caagtgaaat ttgatccacc cttaagaaag gagacagaac
     2401 cacatcatga acttcccgac agtgatggtt ttttggacag ttcagaagaa atatactaca
     2461 ctgcaagatc taatctggat ctgcaactgg aatatggaca aggacaccaa ggtggttact
     2521 ttttaggtgc aaacaagagt cttctcaagt ctgttgaaga agaattgcat cagcgaggac
     2581 acgtcgcaca tttagaagat gatgaaggag atgatgatga atctaaacac tcaactctga
     2641 aagcaaaaat tccacctcct ttgccaccaa agcctaagtc tatcttcata ccacaggaaa
     2701 tgcattctac tgaggatgaa aatcaaggaa caatcaagag atgtcccatg tcagggagcc
     2761 cagcaaagcc atcccacgtt ccacctagac caccacctcc cagattaccc ccacacaaac
     2821 ctgttgcctt aggaaatgga atgagctcct tccagttaaa tggtgaacga gatggctcat
     2881 tatgtcaaca acagaatgaa catagaggca caaacctttc aagaaaagaa aagaaagatg
     2941 taccaaagcc tattagtaat ggtcttcctc caacacctaa agtgcatatg ggtgcatgtt
     3001 tttcaaaagt ttttaatggg tgtcccttga aaattcactg tgcatcatca tggataaacc
     3061 cagatacaag agatcagtac ttgatatttg gtgccgaaga agggatttat accctcaatc
     3121 ttaatgaact tcatgaaaca tcaatggaac agctattccc tcgaaggtgt acatggttgt
     3181 atgtaatgaa caattgcttg ctatcaatat ctggtaaagc ttctcagctt tattcccata
     3241 atttaccagg gctttttgat tatgcaagac aaatgcaaaa gttacctgtt gctattccag
     3301 cacacaaact ccctgacaga atactgccaa ggaaattttc tgtatcagca aaaatccctg
     3361 aaaccaaatg gtgccagaag tgttgtgttg taagaaatcc ttacacgggc cataaatacc
     3421 tatgtggagc acttcagact agcattgttc tattagaatg ggttgaacca atgcagaaat
     3481 ttatgttaat taagcacata gattttccta taccatgtcc acttagaatg tttgaaatgc
     3541 tggtagttcc tgaacaggag taccctttag tttgtgttgg tgtcagtaga ggtagagact
     3601 tcaaccaagt ggttcgattt gagacggtca atccaaattc tacctcttca tggtttacag
     3661 aatcagatac cccacagaca aatgttactc atgtaaccca actggagaga gataccatcc
     3721 ttgtatgctt ggactgttgt ataaaaatag taaatctcca aggaagatta aaatctagca
     3781 ggaaattgtc atcagaactc acctttgatt tccagattga atcaatagtg tgcctacaag
     3841 acagtgtgct agctttctgg aaacatggaa tgcaaggtag aagttttaga tctaatgagg
     3901 taacacaaga aatttcagat agcacaagaa ttttcaggct gcttggatct gacagggtcg
     3961 tggttttgga aagtaggcca actgataacc ccacagcaaa tagcaatttg tacatcctgg
     4021 cgggtcatga aaacagttac tgattgccaa ctttcttgta caaagttggc attataagaa
     4081 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     4141 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     4201 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     4261 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     4321 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     4381 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     4441 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     4501 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     4561 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     4621 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     4681 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4741 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     4801 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4861 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4921 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     4981 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     5041 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     5101 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     5161 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     5221 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     5281 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     5341 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     5401 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     5461 gttacgcgtc gttccact
//
