LOCUS       sequence-168659-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged OSBP PH domain in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pBGPa-CMV-GFP-OSBP PH domain
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(42..61)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(43..61)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(106..309)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(135..155)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        310..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(775..792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attttttcta ccggtgacgt caccatggga agcttgggtc tccctatagt gagtcgtatt
       61 aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata
      121 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
      181 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
      241 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
      301 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
      361 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
      421 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
      481 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
      541 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
      601 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
      661 ctattaataa ctagggccga cgcgctgggc tacgtcttgc tggcgttcgc tgcattaatg
      721 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      781 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      841 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      901 ccagcaa
//
