LOCUS       sequence-16866-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for cloning and expressing your gene of interest. 
            Neomycin selection. This plasmid is not available at Addgene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Morgenstern JP, Land H
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990-06-25"; volume: "18(12)"; pages: "3587-96"
COMMENT     Sequence Label: pBABE-neo
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(44..64)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        49..378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      229..364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(789..808)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gtggtggtac gtaggaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta
       61 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      181 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
      241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
      301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
      361 ggcctaggct tttgcaaaaa gcttcacgct gccgcaagca ctcagggcgc aagggctgct
      421 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
      481 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
      541 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
      601 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
      661 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
      721 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
      781 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
      841 tgttccggct gtcagcgca
//
