LOCUS       sequence-168661-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged SIRT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H,
            Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP,
            Gygi SP, Sinclair DA, Alt FW, Greenberg ME
  TITLE     Stress-dependent regulation of FOXO transcription factors by the 
            SIRT1 deacetylase.
  JOURNAL   Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19.
  PUBMED    14976264
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004-03-26"; volume: "303(5666)"; pages: "2011-5. Epub 2004 
            Feb 19"
COMMENT     Sequence Label: Flag-SIRT1
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(463..486)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttcatcaccg aacagaaggt tatctcggta cccaatcgcc gccgccgccg cctcttcctc
       61 ctcctcgccc tcgtcgtcgt cgtcttcgtc gtacaagttg tcggccagcg gtggctcccg
      121 agatgggccc tgcaggcccg gcccattgtc tccttcccca gccgccgcag tcgcctgggc
      181 ctcttgctcc ccgcctgccg ccgccgcctc tgcctccgcc tcccgccaca gcgccgccgc
      241 cgccgcaccc gggcagcccc tggccgccgc cggcacctca cgctctgggg ccgccccacc
      301 gggctcgccc gggctccgct cgaggccggg accatctctc cgcggcctct tgcggagcgg
      361 ctccccggcg ggggacgacg cggcctccct gtcggccccc gccgccgagg gggagccgcc
      421 gggctgaagg gcgagggccg cctcgtccgc cgaattcccg ggtttatcgt catcgtcctt
      481 gtagtccatc ctgaggatcc gagctcggta ccaagcttag atctcctcca aaaaagcctc
      541 ttcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
      601 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
      661 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
      721 tgcctgctgg gg
//
