LOCUS       sequence-168671-        4605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MTM1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MTM1_p.D298E
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     426..443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(602..629)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(721..807)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     856..878
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     870..887
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     871..887
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    903..1002
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2818..2917)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2934..2953)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2935..2953)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2958..2974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2958..2974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3405..4196
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4289..4605,1..272)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
       61 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      541 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
      601 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
      661 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
      721 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
      781 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      841 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      901 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      961 aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatggct tctgcatcaa
     1021 cttctaaata taattcacac tccttggaga atgagtctat taagaggacg tctcgagatg
     1081 gagtcaatcg agatctcact gaggctgttc ctcgacttcc aggagaaaca ctaatcactg
     1141 acaaagaagt tatttacata tgtcctttca atggccccat taagggaaga gtttacatca
     1201 caaattatcg tctttattta agaagtttgg aaacggattc ttctctaata cttgatgttc
     1261 ctctgggtgt gatctcgaga attgaaaaaa tgggaggcgc gacaagtaga ggagaaaatt
     1321 cctatggtct agatattact tgtaaagaca tgagaaacct gaggttcgct ttgaaacagg
     1381 aaggccacag cagaagagat atgtttgaga tcctcacgag atacgcgttt cccctggctc
     1441 acagtctgcc attatttgca tttttaaatg aagaaaagtt taacgtggat ggatggacag
     1501 tttacaatcc agtggaagaa tacaggaggc agggcttgcc caatcaccat tggagaataa
     1561 cttttattaa taagtgctat gagctctgtg acacttaccc tgctcttttg gtggttccgt
     1621 atcgtgcctc agatgatgac ctccggagag ttgcaacttt taggtcccga aatcgaattc
     1681 cagtgctgtc atggattcat ccagaaaata agacggtcat tgtgcgttgc agtcagcctc
     1741 ttgtcggtat gagtgggaaa cgaaataaag atgatgagaa atatctcgat gttatcaggg
     1801 agactaataa acaaatttct aaactcacca tttatgatgc aagacccagc gtaaatgcag
     1861 tggccaacaa ggcaacagga ggaggatatg aaagtgaaga tgcatatcat aacgccgaac
     1921 ttttcttctt agacattcat aatattcatg ttatgcggga atctttaaaa aaagtgaagg
     1981 acattgttta tcctaatgta gaagaatctc attggttgtc cagtttggag tctactcatt
     2041 ggttagaaca tatcaagctc gttttgacag gagccattca agtagcagac aaagtttctt
     2101 cagggaagag ttcagtgctt gtgcattgca gtgacggatg ggacaggact gctcagctga
     2161 catccttggc catgctgatg ttggatagct tctataggag cattgaaggg ttcgaaatac
     2221 tggtacaaaa aaaatggata agttttggac ataaatttgc atctcgaata ggtcatggtg
     2281 ataaaaacca caccgatgct gaccgttctc ctatttttct ccagtttatt gattgtgtgt
     2341 ggcaaatgtc aaaacagttc cctacagctt ttgaattcaa tgaacaattt ttgattataa
     2401 ttttggatca tctgtatagt tgccgatttg gtactttctt attcaactgt gaatctgctc
     2461 gagaaagaca gaaggttaca gaaaggactg tttctttatg gtcactgata aacagtaata
     2521 aagaaaaatt caaaaacccc ttctatacta aagaaatcaa tcgagtttta tatccagttg
     2581 ccagtatgcg tcacttggaa ctctgggtga attactacat tagatggaac cccaggatca
     2641 agcaacaaca gccgaatcca gtggagcagc gttacatgga gctcttagcc ttacgcgacg
     2701 aatacataaa gcggcttgag gaactgcagc tcgccaactc tgccaagctt tctgatcccc
     2761 caacttcacc ttccagtcct tcgcaaatga tgccccatgt gcaaactcac ttctagttgc
     2821 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2881 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2941 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     3001 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     3061 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     3121 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     3181 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     3241 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     3301 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     3361 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     3421 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3481 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3541 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3601 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3661 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3721 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3781 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3841 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3901 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3961 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     4021 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     4081 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     4141 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     4201 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4261 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4321 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4381 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4441 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4501 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4561 tggactcaag acgatagtta ccggataagg cgcagcggtc gggct
//
