LOCUS       sequence-168675-        4980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing LNX1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_LNX1_p.V564I
FEATURES             Location/Qualifiers
     source          1..4980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2859..2958)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2975..2994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2976..2994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2999..3015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2999..3015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3446..4237
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4330..4918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4819..4838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaaccagc cagagtctgc caacgatcct gaacccctgt gtgcagtgtg
      721 tggccaagcc cactccttgg aggaaaacca cttctacagc tatccagagg aagtggatga
      781 tgacctcatc tgccacatct gcctgcaggc tttgctggac cccctggaca ctccgtgtgg
      841 acacacctac tgcaccctct gcctcaccaa cttcctggtg gagaaggact tctgtcccat
      901 ggaccgcaag cctctggttc tgcagcactg caagaagtcc agcatcctgg tcaacaaact
      961 cctcaacaag ctactggtga cctgcccatt cagggagcac tgcacccagg tgttgcagcg
     1021 ctgtgacctc gagcatcact ttcaaaccag ctgtaaaggt gcctcccact acggcctgac
     1081 caaagatagg aagaggcgct cacaagatgg ctgtccagac ggctgtgcga gcctcacagc
     1141 cacggctccc tccccagagg tttctgcagc tgccaccatc tccttaatga cagacgagcc
     1201 tggcctagac aaccctgcct acgtgtcctc ggcagaggac gggcagccag caatcagccc
     1261 agtggactct ggccggagca accgaactag ggcacggccc tttgagagat ccactattag
     1321 aagcagatca tttaaaaaaa taaatcgagc tttgagtgtt cttcgaagga caaagagcgg
     1381 gagtgcagtt gccaaccatg ccgaccaggg cagggaaaat tctgaaaaca ccactgcccc
     1441 tgaagtcttt ccaaggttgt accacctgat tccagatggt gaaattacca gcatcaagat
     1501 caatcgagta gatcccagtg aaagcctctc tattaggctg gtgggaggta gcgaaacccc
     1561 actggtccat atcattatcc aacacattta tcgtgatggg gtgatcgcca gagacggccg
     1621 gctactgcca ggagacatca ttctaaaggt caacgggatg gacatcagca atgtccctca
     1681 caactacgct gtgcgtctcc tgcggcagcc ctgccaggtg ctgtggctga ctgtgatgcg
     1741 tgaacagaag ttccgcagca ggaacaatgg acaggccccg gatgcctaca gaccccgaga
     1801 tgacagcttt catgtgattc tcaacaaaag tagccccgag gagcagcttg gaataaaact
     1861 ggtgcgcaag gtggatgagc ctggggtttt catcttcaat gtgctggatg gcggtgtggc
     1921 atatcgacat ggtcagcttg aggagaatga ccgtgtgtta gccatcaatg gacatgatct
     1981 tcgatatggc agcccagaaa gtgcggctca tctgattcag gccagtgaaa gacgtgttca
     2041 cctcgtcgtg tcccgccagg ttcggcagcg gagccctgac atctttcagg aagccggctg
     2101 gaacagcaat ggcagctggt ccccagggcc aggggagagg agcaacactc ccaagcccct
     2161 ccatcctaca attacttgtc atgagaaggt ggtaaatatc caaaaagacc ccggtgaatc
     2221 tctcggcatg accgtcgcag ggggagcatc acatagagaa tgggatttgc ctatctatgt
     2281 catcagtgtt gagcccggag gagtcataag cagagatgga agaataaaaa caggtgacat
     2341 tttgttgaat gtggatggga tcgaactgac agaggtcagc cggagtgagg cagtggcatt
     2401 attgaaaaga acatcatcct cgatagtact caaagctttg gaagtcaaag agtatgagcc
     2461 ccaggaagac tgcagcagcc cagcagccct ggactccaac cacaacatgg ccccacccag
     2521 tgactggtcc ccatcctggg tcatgtggct ggaattacca cggtgcttgt ataactgtaa
     2581 agatattgta ttacgaagaa acacagctgg aagtctgggc ttctgcattg taggaggtta
     2641 tgaagaatac aatggaaaca aacctttttt catcaaatcc attgttgaag gaacaccagc
     2701 atacaatgat ggaagaatta gatgtggtga tattcttctt gctgtcaatg gtagaagtac
     2761 atcaggaatg atacatgctt gcttggcaag actgctgaaa gaacttaaag gaagaattac
     2821 tctaactatt gtttcttggc ctggcacttt tttatgattg ccaactttct tgtacaaagt
     2881 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2941 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3001 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3061 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     3121 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     3181 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     3241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     3301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     3361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     3421 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     3481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     4021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     4081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     4141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     4201 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     4261 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
