LOCUS       sequence-168676-        4960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAPK6, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAPK6_p.D387N
FEATURES             Location/Qualifiers
     source          1..4960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(932..959)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1051..1137)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1186..1208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1200..1217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1201..1217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1233..1332
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3503..3602)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3619..3638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3620..3638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3643..3659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3643..3659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4090..4881
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
      901 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      961 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     1021 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     1081 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     1141 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     1201 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
     1261 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
     1321 aaaaagttgg caccatggca gagaaaattt gaaagtctca tgaacattca tggttttgat
     1381 ctgggttcta ggtatatgga cttaaaacca ttgggttgtg gaggcaatgg cttggttttt
     1441 tctgctgtag acaatgactg tgacaaaaga gtagccatca agaaaattgt ccttactgat
     1501 ccccagagtg tcaaacatgc tctacgtgaa atcaaaatta ttagaagact tgaccatgat
     1561 aacattgtga aagtgtttga gattcttggt cccagtggaa gccaattaac agacgatgtg
     1621 ggctctctta cggaactgaa cagtgtttac attgttcagg agtacatgga gacagacttg
     1681 gctaatgtgc tggagcaggg ccctttactg gaagagcatg ccaggctttt catgtatcag
     1741 ctgctacggg ggctcaagta tattcactct gcaaatgtac tgcacagaga tctcaaacca
     1801 gctaatcttt tcattaatac ggaagacttg gtgctgaaga taggtgactt tggtcttgca
     1861 cggatcatgg atcctcatta ttcccataag ggtcatcttt ctgaaggatt ggttactaaa
     1921 tggtacagat ctccacgtct tttactttct cctaataatt atactaaagc cattgacatg
     1981 tgggctgcag gctgcatctt tgctgaaatg ctgactggta aaaccctttt tgcaggtgca
     2041 catgaacttg aacagatgca gctgatttta gaatctattc ctgttgtaca tgaggaagat
     2101 cgtcaggagc ttctcagcgt aattccagtt tacattagaa atgacatgac tgagccacac
     2161 aaacctttaa ctcagctgct tccaggaatt agtcgagaag cagtggattt cctggaacaa
     2221 attttgacat ttagccccat ggatcggtta acagcagaag aagcactctc ccatccttac
     2281 atgagcatat attcttttcc aatggatgag ccaatttcaa gccatccttt tcatattgaa
     2341 gatgaagttg atgatatttt gcttatggat gaaactcaca gtcacattta taactgggaa
     2401 aggtatcatg attgtcagtt ttcagagcat gattggcctg tacataacaa ctttgatatt
     2461 gatgaagttc agcttgatcc aagagctctg tccaatgtca ctgatgaaga agaagtacaa
     2521 gttgatcccc gaaaatattt ggatggagat cgggaaaagt atctggagga tcctgctttt
     2581 gataccaatt actctactga gccttgttgg caatactcag atcatcatga aaacaaatat
     2641 tgtgatctgg agtgtagcca tacttgtaac tacaaaacga ggtcatcatc atatttagat
     2701 aacttagttt ggagagagag tgaagttaac cattactatg aacccaagct tattatagat
     2761 ctttccaatt ggaaagaaca aagcaaagaa aaatctgata agaaaggcaa atcaaaatgt
     2821 gaaaggaatg gattggttaa agcccagata gcgctagagg aagcatcaca gcaactggct
     2881 ggaaaagaaa gggaaaagaa tcagggattt gattttgatt cctttattgc aggaactatt
     2941 cagcttagtt cccagcatga gcctactgat gttgttgata aattaaatga cttgaatagc
     3001 tcagtgtccc aactagaatt gaaaagtttg atatcaaagt cagtaagcca agaaaaacag
     3061 gaaaaaggaa tggcaaatct ggctcaatta gaagccttgt accagtcttc ttgggacagc
     3121 cagtttgtga gtggtgggga ggactgtttt ttcataaatc agttttgtga ggtaaggaag
     3181 gatgaacaag ttgagaagga aaacacttac actagttact tggacaagtt ctttagcagg
     3241 aaagaagata ctgaaatgct agaaactgag ccagtagagg atgggaagct tggggagaga
     3301 ggacatgagg aaggatttct gaacaacagt ggggagttcc tctttaacaa gcagctcgag
     3361 tccataggca tcccacagtt tcacagtcca gttgggtcac cacttaagtc aatacaggcc
     3421 acattaacac cttctgctat gaaatcttcc cctcaaattc ctcatcaaac atacagcagc
     3481 attctgaaac atctgaactg attgccaact ttcttgtaca aagttggcat tataagaaag
     3541 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     3601 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     3661 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     3721 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     3781 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     3841 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     3901 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     3961 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     4021 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     4081 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     4141 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     4201 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     4261 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     4321 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     4381 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     4441 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     4501 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     4561 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     4621 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     4681 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     4741 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     4801 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     4861 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     4921 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
//
