LOCUS       sequence-168677-        4797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SCEL ,part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SCEL_p.S10Y
FEATURES             Location/Qualifiers
     source          1..4797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2676..2775)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2792..2811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2793..2811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2816..2832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2816..2832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3263..4054
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4147..4735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4636..4655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa
      661 agttggcacc atgtccaatg ttaccttgag aaaaatgtat cccacaggaa atgagatgaa
      721 gagcaccact cagggaacca cacggaagca gcaggatttt cacgaggtga acaaaagaag
      781 aactttctta caggataaca gttggataaa gaaacgccct gaagaagaaa aagatgaaaa
      841 ttacggtagg gtggtgctca accgacataa ttcccatgat gcattggaca ggaaagtaaa
      901 tgagagagat gtgccaaaag ctacaattag tcggtacagt tctgatgaca ctttggacag
      961 gatctcagac agaaatgatg ctgctaaaac atataaggcc aataccttgg ataaccaact
     1021 aaccaatagg agcatgtccg tgtttagatc actggaagta acaaagttgc aacctggcgg
     1081 ttcattgaat gccaacacct ccaacaccat agcatccact tctgctacta ctcctgtaaa
     1141 gaagaagagg cagtcctggt ttccaccgcc ccctccaggt tacaatgcct cttcgagcac
     1201 aggaaccagg agacgggaac caggtgttca ccctccaata cctccaaagc ccagttctcc
     1261 tgtttcttct actaaccagc tgagacagga taataggcag atacatccac ctaaaccagg
     1321 tgtatataca gaaaccaaca gatctgctga aagaaatata agtgaagaat tggataatct
     1381 catcaaaatg aacaaaagct tgaataggaa tcaaggtctt gatagtctct tcagagcaaa
     1441 tccaaaggta gaagaaagag agaaaagagc caaaagcctt gaaagtctca tctatatgag
     1501 tacccggaca gataaagatg gcaaaggaat ccaaagcctt ggaagtccga ttaaagttaa
     1561 tcaaaggact gacaaaaatg agaaaggaag acaaaatctc gaatctgttg ctaaagtgaa
     1621 tgccaggatg aataaaacga gcagaagaag tgaagacctt gataatgcta ctgaagtaaa
     1681 tcccaaagga catgaaaata ccactggaaa aaaagacctt gatgggctta ttaaagtgga
     1741 tcctgaaaca aataaaaata ttacgagggg ccagagcctt gataatctca tcaaagtgac
     1801 ccctgaagta aagagaagta accaaggttc caaagacctt aataacttca tcaaagtgta
     1861 tccaggaaca gaaaaaagta ctgaaggggg ccaaagtctc gacagcctca ttaaagtgac
     1921 tcctgaaaga aacagaacta accaagggaa ccaagacttg gaaaatctta tcaaagtgat
     1981 cccttcagca aacaaaagca gtgaacaagg tcttgatgaa catattaatg tcagccccaa
     2041 agctgtcaaa aacactgatg gaaaacaaga tcttgataaa ctcatcaagg tgaatcctga
     2101 aattttcaca aacaaccaaa gaaaccaaga tcttgctaac ctcatcaaag taaatcctgc
     2161 agtaatcaga aacaatcaga gccaagactt ggacaatctt attaaagtga aaccttcagc
     2221 tcttagaaac actaatcgag accagaacct ggaaaattta attgaagtaa attctcatgt
     2281 gtctgaaaac aagaatggaa gctctaacac tggagcaaag caggcaggac cacaggatac
     2341 tgttgtgtac acaaggacat atgtggagaa tagtaaatca cccaaggatg gatatcagga
     2401 gaatatctct ggaaaataca tacaaactgt ttattcaact tctgataggt ctgtcattga
     2461 aagagatatg tgcacttact gccgaaaacc cttgggtgta gaaactaaaa tgattttaga
     2521 tgaattacaa atttgctgcc attctacttg ctttaagtgt gaaatatgca agcagccttt
     2581 ggaaaatcta caagcgggtg atagtatttg gatttataga cagacaatac actgtgaacc
     2641 ttgctactct aaaattatgg caaagtggat tccataccca actttcttgt acaaagttgg
     2701 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2761 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2821 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2881 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     2941 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3001 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3061 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3121 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3181 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3241 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3301 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3361 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3421 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3481 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3541 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3601 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3661 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3721 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3781 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     3841 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     3901 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     3961 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4021 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4081 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4261 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
