LOCUS       sequence-168681-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.R535Q
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     763..780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(939..966)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1058..1144)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1193..1215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1207..1224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1208..1224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1240..1339
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1342..3684
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPQLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3689..3788)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3805..3824)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3806..3824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3829..3845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3829..3845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4276..5067
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      841 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      901 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
      961 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     1021 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     1081 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     1141 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     1201 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
     1261 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
     1321 ttgtacaaaa aagttggcac catggctact caagctgatt tgatggagtt ggacatggcc
     1381 atggaaccag acagaaaagc ggctgttagt cactggcagc aacagtctta cctggactct
     1441 ggaatccatt ctggtgccac taccacagct ccttctctga gtggtaaagg caatcctgag
     1501 gaagaggatg tggatacctc ccaagtcctg tatgagtggg aacagggatt ttctcagtcc
     1561 ttcactcaag aacaagtagc tgatattgat ggacagtatg caatgactcg agctcagagg
     1621 gtacgagctg ctatgttccc tgagacatta gatgagggca tgcagatccc atctacacag
     1681 tttgatgctg ctcatcccac taatgtccag cgtttggctg aaccatcaca gatgctgaaa
     1741 catgcagttg taaacttgat taactatcaa gatgatgcag aacttgccac acgtgcaatc
     1801 cctgaactga caaaactgct aaatgacgag gaccaggtgg tggttaataa ggctgcagtt
     1861 atggtccatc agctttctaa aaaggaagct tccagacacg ctatcatgcg ttctcctcag
     1921 atggtgtctg ctattgtacg taccatgcag aatacaaatg atgtagaaac agctcgttgt
     1981 accgctggga ccttgcataa cctttcccat catcgtgagg gcttactggc catctttaag
     2041 tctggaggca ttcctgccct ggtgaaaatg cttggttcac cagtggattc tgtgttgttt
     2101 tatgccatta caactctcca caacctttta ttacatcaag aaggagctaa aatggcagtg
     2161 cgtttagctg gtgggctgca gaaaatggtt gccttgctca acaaaacaaa tgttaaattc
     2221 ttggctatta cgacagactg ccttcaaatt ttagcttatg gcaaccaaga aagcaagctc
     2281 atcatactgg ctagtggtgg accccaagct ttagtaaata taatgaggac ctatacttac
     2341 gaaaaactac tgtggaccac aagcagagtg ctgaaggtgc tatctgtctg ctctagtaat
     2401 aagccggcta ttgtagaagc tggtggaatg caagctttag gacttcacct gacagatcca
     2461 agtcaacgtc ttgttcagaa ctgtctttgg actctcagga atctttcaga tgctgcaact
     2521 aaacaggaag ggatggaagg tctccttggg actcttgttc agcttctggg ttcagatgat
     2581 ataaatgtgg tcacctgtgc agctggaatt ctttctaacc tcacttgcaa taattataag
     2641 aacaagatga tggtctgcca agtgggtggt atagaggctc ttgtgcgtac tgtccttcgg
     2701 gctggtgaca gggaagacat cactgagcct gccatctgtg ctcttcgtca tctgaccagc
     2761 cgacaccaag aagcagagat ggcccagaat gcagttcgcc ttcactatgg actaccagtt
     2821 gtggttaagc tcttacaccc accatcccac tggcctctga taaaggctac tgttggattg
     2881 attcgaaatc ttgccctttg tcccgcaaat catgcacctt tgcgtgagca gggtgccatt
     2941 ccacaactag ttcagttgct tgttcgtgca catcaggata cccagcgccg tacgtccatg
     3001 ggtgggacac agcagcaatt tgtggagggg gtccgcatgg aagaaatagt tgaaggttgt
     3061 accggagccc ttcacatcct agctcgggat gttcacaacc gaattgttat cagaggacta
     3121 aataccattc cattgtttgt gcagctgctt tattctccca ttgaaaacat ccaaagagta
     3181 gctgcagggg tcctctgtga acttgctcag gacaaggaag ctgcagaagc tattgaagct
     3241 gagggagcca cagctcctct gacagagtta cttcactcta ggaatgaagg tgtggcgaca
     3301 tatgcagctg ctgttttgtt ccgaatgtct gaggacaagc cacaagatta caagaaacgg
     3361 ctttcagttg agctgaccag ctctctcttc agaacagagc caatggcttg gaatgagact
     3421 gctgatcttg gacttgatat tggtgcccag ggagaacccc ttggatatcg ccaggatgat
     3481 cctagctatc gttcttttca ctctggtgga tatggccagg atgccttggg tatggacccc
     3541 atgatggaac atgagatggg tggccaccac cctggtgctg actatccagt tgatgggctg
     3601 ccagatctgg ggcatgccca ggacctcatg gatgggctgc ctccaggtga cagcaatcag
     3661 ctggcctggt ttgatactga cctgtgattg ccaactttct tgtacaaagt tggcattata
     3721 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     3781 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     3841 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     3901 atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     3961 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     4021 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     4081 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     4141 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     4201 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     4261 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     4321 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     4381 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     4441 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     4501 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     4561 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     4621 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     4681 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     4741 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     4801 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     4861 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     4921 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     4981 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     5041 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     5101 gtgagttacg cgtcgttcca ctgagcgtca gaccccgta
//
