LOCUS       sequence-168685-        6558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2_p.R897Q
FEATURES             Location/Qualifiers
     source          1..6558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      54..642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     543..562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     796..813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(972..999)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1091..1177)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1226..1248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1240..1257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1241..1257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1273..1372
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5141..5240)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5257..5276)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5258..5276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5281..5297)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5281..5297)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5728..6519
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
       61 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      901 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
      961 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     1021 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     1081 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     1141 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     1201 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     1261 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
     1321 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg catggagctg
     1381 gcggccttgt gccgctgggg gctcctcctc gccctcttgc cccccggagc cgcgagcacc
     1441 caagtgtgca ccggcacaga catgaagctg cggctccctg ccagtcccga gacccacctg
     1501 gacatgctcc gccacctcta ccagggctgc caggtggtgc agggaaacct ggaactcacc
     1561 tacctgccca ccaatgccag cctgtccttc ctgcaggata tccaggaggt gcagggctac
     1621 gtgctcatcg ctcacaacca agtgaggcag gtcccactgc agaggctgcg gattgtgcga
     1681 ggcacccagc tctttgagga caactatgcc ctggccgtgc tagacaatgg agacccgctg
     1741 aacaatacca cccctgtcac aggggcctcc ccaggaggcc tgcgggagct gcagcttcga
     1801 agcctcacag agatcttgaa aggaggggtc ttgatccagc ggaaccccca gctctgctac
     1861 caggacacga ttttgtggaa ggacatcttc cacaagaaca accagctggc tctcacactg
     1921 atagacacca accgctctcg ggcctgccac ccctgttctc cgatgtgtaa gggctcccgc
     1981 tgctggggag agagttctga ggattgtcag agcctgacgc gcactgtctg tgccggtggc
     2041 tgtgcccgct gcaaggggcc actgcccact gactgctgcc atgagcagtg tgctgccggc
     2101 tgcacgggcc ccaagcactc tgactgcctg gcctgcctcc acttcaacca cagtggcatc
     2161 tgtgagctgc actgcccagc cctggtcacc tacaacacag acacgtttga gtccatgccc
     2221 aatcccgagg gccggtatac attcggcgcc agctgtgtga ctgcctgtcc ctacaactac
     2281 ctttctacgg acgtgggatc ctgcaccctc gtctgccccc tgcacaacca agaggtgaca
     2341 gcagaggatg gaacacagcg gtgtgagaag tgcagcaagc cctgtgcccg agtgtgctat
     2401 ggtctgggca tggagcactt gcgagaggtg agggcagtta ccagtgccaa tatccaggag
     2461 tttgctggct gcaagaagat ctttgggagc ctggcatttc tgccggagag ctttgatggg
     2521 gacccagcct ccaacactgc cccgctccag ccagagcagc tccaagtgtt tgagactctg
     2581 gaagagatca caggttacct atacatctca gcatggccgg acagcctgcc tgacctcagc
     2641 gtcttccaga acctgcaagt aatccgggga cgaattctgc acaatggcgc ctactcgctg
     2701 accctgcaag ggctgggcat cagctggctg gggctgcgct cactgaggga actgggcagt
     2761 ggactggccc tcatccacca taacacccac ctctgcttcg tgcacacggt gccctgggac
     2821 cagctctttc ggaacccgca ccaagctctg ctccacactg ccaaccggcc agaggacgag
     2881 tgtgtgggcg agggcctggc ctgccaccag ctgtgcgccc gagggcactg ctggggtcca
     2941 gggcccaccc agtgtgtcaa ctgcagccag ttccttcggg gccaggagtg cgtggaggaa
     3001 tgccgagtac tgcaggggct ccccagggag tatgtgaatg ccaggcactg tttgccgtgc
     3061 caccctgagt gtcagcccca gaatggctca gtgacctgtt ttggaccgga ggctgaccag
     3121 tgtgtggcct gtgcccacta taaggaccct cccttctgcg tggcccgctg ccccagcggt
     3181 gtgaaacctg acctctccta catgcccatc tggaagtttc cagatgagga gggcgcatgc
     3241 cagccttgcc ccatcaactg cacccactcc tgtgtggacc tggatgacaa gggctgcccc
     3301 gccgagcaga gagccagccc tctgacgtcc atcatctctg cggtggttgg cattctgctg
     3361 gtcgtggtct tgggggtggt ctttgggatc ctcatcaagc gacggcagca gaagatccgg
     3421 aagtacacga tgcggagact gctgcaggaa acggagctgg tggagccgct gacacctagc
     3481 ggagcgatgc ccaaccaggc gcagatgcgg atcctgaaag agacggagct gaggaaggtg
     3541 aaggtgcttg gatctggcgc ttttggcaca gtctacaagg gcatctggat ccctgatggg
     3601 gagaatgtga aaattccagt ggccatcaaa gtgttgaggg aaaacacatc ccccaaagcc
     3661 aacaaagaaa tcttagacga agcatacgtg atggctggtg tgggctcccc atatgtctcc
     3721 cgccttctgg gcatctgcct gacatccacg gtgcagctgg tgacacagct tatgccctat
     3781 ggctgcctct tagaccatgt ccgggaaaac cgcggacgcc tgggctccca ggacctgctg
     3841 aactggtgta tgcagattgc caaggggatg agctacctgg aggatgtgcg gctcgtacac
     3901 agggacttgg ccgctcggaa cgtgctggtc aagagtccca accatgtcaa aattacagac
     3961 ttcgggctgg ctcggctgct ggacattgac gagacagagt accatgcaga tgggggcaag
     4021 gtgcccatca agtggatggc gctggagtcc attctccgcc agcggttcac ccaccagagt
     4081 gatgtgtgga gttatggtgt gactgtgtgg gagctgatga cttttggggc caaaccttac
     4141 gatgggatcc cagcccggga gatccctgac ctgctggaaa agggggagcg gctgccccag
     4201 ccccccatct gcaccattga tgtctacatg atcatggtca aatgttggat gattgactct
     4261 gaatgtcggc caagattccg ggagttggtg tctgaattct cccgcatggc cagggacccc
     4321 cagcgctttg tggtcatcca gaatgaggac ttgggcccag ccagtccctt ggacagcacc
     4381 ttctaccgct cactgctgga ggacgatgac atgggggacc tggtggatgc tgaggagtat
     4441 ctggtacccc agcagggctt cttctgtcca gaccctgccc cgggcgctgg gggcatggtc
     4501 caccacaggc accgcagctc atctaccagg agtggcggtg gggacctgac actagggctg
     4561 gagccctctg aagaggaggc ccccaggtct ccactggcac cctccgaagg ggctggctcc
     4621 gatgtatttg atggtgacct gggaatgggg gcagccaagg ggctgcaaag cctccccaca
     4681 catgacccca gccctctaca gcggtacagt gaggacccca cagtacccct gccctctgag
     4741 actgatggct acgttgcccc cctgacctgc agcccccagc ctgaatatgt gaaccagcca
     4801 gatgttcggc cccagccccc ttcgccccga gagggccctc tgcctgctgc ccgacctgct
     4861 ggtgccactc tggaaaggcc caagactctc tccccaggga agaatggggt cgtcaaagac
     4921 gtttttgcct ttgggggtgc cgtggagaac cccgagtact tgacacccca gggaggagct
     4981 gcccctcagc cccaccctcc tcctgccttc agcccagcct tcgacaacct ctattactgg
     5041 gaccaggacc caccagagcg gggggctcca cccagcacct tcaaagggac acctacggca
     5101 gagaacccag agtacctggg tctggacgtg ccagtgtgat tgccaacttt cttgtacaaa
     5161 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     5221 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     5281 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     5341 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     5401 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     5461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     5521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     5581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     5641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     5701 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     5761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     5821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     5881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     5941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     6001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     6061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     6121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     6181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     6241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     6301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     6361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     6421 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     6481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     6541 aaatccctta acgtgagt
//
