LOCUS       sequence-168686-        4980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BAP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BAP1_p.D75G
FEATURES             Location/Qualifiers
     source          1..4980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    1859..1870
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(2859..2958)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2975..2994)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2976..2994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2999..3015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2999..3015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3446..4237
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4330..4918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4819..4838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaataagg gctggctgga gctggagagc gacccaggcc tcttcaccct
      721 gctcgtggaa gatttcggtg tcaagggggt gcaagtggag gagatctacg accttcagag
      781 caaatgtcag ggccctgtat atggatttat cttcctgttc aaatggatcg aagagcgccg
      841 gtcccggcga aaggtctcta ccttggtgga tgatacgtcc gtgattgatg atggtattgt
      901 gaataacatg ttctttgccc accagctgat acccaactct tgtgcaactc atgccttgct
      961 gagcgtgctc ctgaactgca gcagcgtgga cctgggaccc accctgagtc gcatgaagga
     1021 cttcaccaag ggttttagcc ctgagagcaa aggatatgcg attggcaatg ccccggagtt
     1081 ggccaaggcc cataatagcc atgccaggcc cgagccacgc cacctccctg agaagcagaa
     1141 tggccttagt gcagtgcgga ccatggaggc gttccacttt gtcagctatg tgcctatcac
     1201 aggccggctc tttgagctgg atgggctgaa ggtctacccc attgaccatg ggccctgggg
     1261 ggaggacgag gagtggacag acaaggcccg gcgggtcatc atggagcgta tcggcctcgc
     1321 cactgcaggg gagccctacc acgacatccg cttcaacctg atggcagtgg tgcccgaccg
     1381 caggatcaag tatgaggcca ggctgcatgt gctgaaggtg aaccgtcaga cagtactaga
     1441 ggctctgcag cagctgataa gagtaacaca gccagagctg attcagaccc acaagtctca
     1501 agagtcacag ctgcctgagg agtccaagtc agccagcaac aagtccccgc tggtgctgga
     1561 agcaaacagg gcccctgcag cctctgaggg caaccacaca gatggtgcag aggaggcggc
     1621 tggttcatgc gcacaagccc catcccacag ccctcccaac aaacccaagc tagtggtgaa
     1681 gcctccaggc agcagcctca atggggttca ccccaacccc actcccattg tccagcggct
     1741 gccggccttt ctagacaatc acaattatgc caagtccccc atgcaggagg aagaagacct
     1801 ggcggcaggt gtgggccgca gccgagttcc agtccgccca ccccagcagt actcagatga
     1861 tgaggatgac tatgaggatg acgaggagga tgacgtgcag aacaccaact ctgcccttag
     1921 gtataagggg aagggaacag ggaagccagg ggcattgagc ggttctgctg atgggcaact
     1981 gtcagtgctg cagcccaaca ccatcaacgt cttggctgag aagctcaaag agtcccagaa
     2041 ggacctctca attcctctgt ccatcaagac tagcagcggg gctgggagtc cggctgtggc
     2101 agtgcccaca cactcgcagc cctcacccac ccccagcaat gagagtacag acacggcctc
     2161 tgagatcggc agtgctttca actcgccact gcgctcgcct atccgctcag ccaacccgac
     2221 gcggccctcc agccctgtca cctcccacat ctccaaggtg ctttttggag aggatgacag
     2281 cctgctgcgt gttgactgca tacgctacaa ccgtgctgtc cgtgatctgg gtcctgtcat
     2341 cagcacaggc ctgctgcacc tggctgagga tggggtgctg agtcccctgg cgctgacaga
     2401 gggtgggaag ggttcctcgc cctccatcag accaatccaa ggcagccagg ggtccagcag
     2461 cccagtggag aaggaggtcg tggaagccac ggacagcaga gagaagacgg ggatggtgag
     2521 gcctggcgag cccttgagtg gggagaaata ctcacccaag gagctgctgg cactgctgaa
     2581 gtgtgtggag gctgagattg caaactatga ggcgtgcctc aaggaggagg tagagaagag
     2641 gaagaagttc aagattgatg accagagaag gacccacaac tacgatgagt tcatctgcac
     2701 ctttatctcc atgctggctc aggaaggcat gctggccaac ctagtggagc agaacatctc
     2761 cgtgcggcgg cgccaagggg tcagcatcgg ccggctccac aagcagcgga agcctgaccg
     2821 gcggaaacgc tctcgcccct acaaggccaa gcgccagtgc ccaactttct tgtacaaagt
     2881 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2941 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3001 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3061 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     3121 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     3181 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     3241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     3301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     3361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     3421 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     3481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     4021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     4081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     4141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     4201 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     4261 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
