LOCUS       sequence-168689-        5133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTPN12 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTPN12_p.E57D
FEATURES             Location/Qualifiers
     source          1..5133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3012..3111)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3128..3147)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3129..3147)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3152..3168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3152..3168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3599..4390
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4483..5071
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4972..4991
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagcaag tggagatcct gaggaaattc atccagaggg tccaggccat
      721 gaagagtcct gaccacaatg gggaggacaa cttcgcccgg gacttcatgc ggttaagaag
      781 attgtctacc aaatatagaa cagaaaagat atatcccaca gccactggag aaaaagaaga
      841 caatgttaaa aagaacagat acaaggacat actgccattt gatcacagcc gagttaaatt
      901 gacattaaag actccttcac aagattcaga ctatatcaat gcaaatttta taaagggcgt
      961 ctatgggcca aaagcatatg tagcaactca aggaccttta gcaaatacag taatagattt
     1021 ttggaggatg atatgggagt ataatgttgt gatcattgta atggcctgcc gagaatttga
     1081 gatgggaagg aaaaaatgtg agcgctattg gcctttgtat ggagaagacc ccataacgtt
     1141 tgcaccattt aaaatttctt gtgaggatga acaagcaaga acagactact tcatcaggac
     1201 actcttactt gaatttcaaa atgaatctcg taggctgtat cagtttcatt atgtgaactg
     1261 gccagaccat gatgttcctt catcatttga ttctattctg gacatgataa gcttaatgag
     1321 gaaatatcaa gaacatgaag atgttcctat ttgtattcat tgcagtgcag gctgtggaag
     1381 aacaggtgcc atttgtgcca tagattatac gtggaattta ctaaaagctg ggaaaatacc
     1441 agaggaattt aatgtattta atttaataca agaaatgaga acacaaaggc attctgcagt
     1501 acaaacaaag gagcaatatg aacttgttca tagagctatt gcccaactgt ttgaaaaaca
     1561 gctacaacta tatgaaattc atggagctca gaaaattgct gatggagtga atgaaattaa
     1621 cactgaaaac atgatcagct ccatagagcc tgaaaaacaa gattctcctc ctccaaaacc
     1681 accaaggacc cgcagttgcc ttgttgaagg ggatgctaaa gaagaaatac tgcagccacc
     1741 ggaacctcat ccagtgccac ccatcttgac accttctccc ccttcagctt ttccaacagt
     1801 cactactgtg tggcaggaca atgatagata ccatccaaag ccagtgttgc atatggtttc
     1861 atcagaacaa cattcagcag acctcaacag aaactatagt aaatcaacag aacttccagg
     1921 gaaaaatgaa tcaacaattg aacagataga taaaaaattg gaacgaaatt taagttttga
     1981 gattaagaag gtccctctcc aagagggacc aaaaagtttt gatgggaaca cacttttgaa
     2041 taggggacat gcaattaaaa ttaaatctgc ttcaccttgt atagctgata aaatctctaa
     2101 gccacaggaa ttaagttcag atctaaatgt cggtgatact tcccagaatt cttgtgtgga
     2161 ctgcagtgta acacaatcaa acaaagtttc agttactcca ccagaagaat cccagaattc
     2221 agacacacct ccaaggccag accgcttgcc tcttgatgag aaaggacatg taacgtggtc
     2281 atttcatgga cctgaaaatg ccatacccat acctgattta tctgaaggca attcctcaga
     2341 tatcaactat caaactagga aaactgtgag tttaacacca agtcctacaa cacaagttga
     2401 aacacctgat cttgtggatc atgataacac ttcaccactc ttcagaacac ccctcagttt
     2461 tactaatcca cttcactctg atgactcaga ctcagatgaa agaaactctg atggtgctgt
     2521 gacccagaat aaaactaata tttcaacagc aagtgccaca gtttctgctg ccactagtac
     2581 tgaaagcatt tctactagga aagtattgcc aatgtccatt gctagacata atatagcagg
     2641 aacaacacat tcaggtgctg aaaaagatgt tgatgttagt gaagattcac ctcctcccct
     2701 acctgaaaga actcctgaat cgtttgtgtt agcaagtgaa cataatacac ctgtaagatc
     2761 ggaatggagt gaacttcaaa gtcaggaacg atctgaacaa aaaaagtctg aaggcttgat
     2821 aacctctgaa aatgagaaat gtgatcatcc agcgggaggt attcactatg aaatgtgcat
     2881 agaatgtcca cctactttca gtgacaagag agaacaaata tcagaaaatc caacagaagc
     2941 cacagatatt ggttttggta atcgatgtgg aaaacccaaa ggaccaagag atccaccttc
     3001 agaatggaca tgcccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     3061 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     3121 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     3181 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     3241 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     3301 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     3361 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     3421 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     3481 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     3541 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     3601 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     3661 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     3721 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     3781 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     3841 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3901 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3961 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     4021 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     4081 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     4141 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     4201 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     4261 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     4321 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     4381 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     4441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4501 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4561 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4621 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4681 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4801 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4861 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5041 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5101 cctggccttt tgctggcctt ttgctcacat gtt
//
