LOCUS       sequence-168692-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM
  TITLE     DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that 
            enhances cell motility through a GAP-dependent mechanism.
  JOURNAL   J Biol Chem. 2002 Mar 8. 277(10):7962-9.
  PUBMED    11773070
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Mar 8. 277(10):7962-9."
COMMENT     Sequence Label: pcDNA3 HA Arf6 ActQ67L
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             642..668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(743..761)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(744..761)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(781..798)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    787..898
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcatgg
      121 ggaaggtgct atccaaaatc ttcgggaaca aggaaatgcg gatcctcatg ttgggcctgg
      181 acgcggccgg caagacaaca atcctgtaca agttgaagct gggccagtcg gtgaccacca
      241 ttcccactgt gggtttcaac gtggagacgg tgacttacaa aaatgtcaag ttcaacgtat
      301 gggatgtggg cggtctagac aagatccggc cgctctggcg gcattactac actgggaccc
      361 aaggtctcat cttcgtagtg gactgcgccg accgcgaccg catcgatgag gctcgccagg
      421 agctgcaccg cattatcaat gaccgggaga tgagggacgc cataatcctc atcttcgcca
      481 acaagcagga cctgcccgat gccatgaaac cccacgagat ccaggagaaa ctgggcctga
      541 cccggattcg ggacaggaac tggtatgtgc agccctcctg tgccacctca ggggacggac
      601 tctatgaggg gctcacatgg ttaacctcta actacaaatc ttacccatac gatgttccag
      661 attacgctta atagtctaga cagctgagat ctgatatcca tcacactggc ggccgctcga
      721 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
      781 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      841 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      901 attgtctgag taggtgtcat tctattctg
//
