LOCUS       sequence-168695-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pCMV-GAL4
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             98..538
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagtta agcttggtac cgagctcgga tcccaacatg aagctactgt cttctatcga
      121 acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg
      181 cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc
      241 gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt
      301 tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga
      361 tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac
      421 agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag
      481 tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgat
      541 tgactcggca gctcatcatg ataactccac aattccgttg gattttatgc ccagggatgc
      601 tcttcatgga tttgattggt ctgaagagga tgacatgtcg gatggcttgc ccttcctgaa
      661 aacggacccc aacaataatg ggttctttgg cgacggttct ctcttatgta ttcttcgatc
      721 tattggcttt aaaccggaaa attacacgaa ctctaacgtt aacaggctcc cgaccatgat
      781 tacggataga tacacgttgg cttctagatc cacaacatcc cgtttacttc aaagttatct
      841 caataatttt cacccctact gccctatcgt gcactcaccg acgctaatga tgttgtataa
      901 taaccagat
//
