LOCUS       sequence-168712-        7072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sLucopt luciferase from the constitive Bacillus subtilis 
            promoter Pveg in Clostridium difficile.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7072)
  TITLE     Smits lab Constitutively secreted luciferase plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAP84
FEATURES             Location/Qualifiers
     source          1..7072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(343..714)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            747..856
                     /note="incP origin of transfer"
     primer_bind     complement(2045..2062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2216..2804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2296..2315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcctataa gttttaataa aactttaaat agaaaaaggc ttctctcatg agaagtcttt
       61 tttatttaaa ataaatataa aataaaatag aggctataaa tagcctctat tttatgtgag
      121 aaatccctaa ataaaaagat gccagtgtgc tggaattcgc ccttaggctg cgctagggac
      181 ctctttagct ccttggaagc tgtcagtagt aggggatcgg tcttgccttg ctcgtcggtg
      241 atgtacttca ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg
      301 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg
      361 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag
      421 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag
      481 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg
      541 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga
      601 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
      661 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt
      721 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag
      781 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
      841 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
      901 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
      961 gaccccgaag caggaagggc gaattctgca gatctgaccg gtctctgaaa atataaaaac
     1021 cacagattga tactaaaacc ttggttgtgt tgcttttcgg ggcttaaatc aaggaaaaat
     1081 ccttgtttta agcctttcaa aaagaaacac aaggtctttg tactaacctg tggttatgta
     1141 taaaattgta gattttaggg taacaaaaaa caccgtattt ctacgatgtt tttgcttaaa
     1201 tacttgtttt tagttacaga caaacctgaa gttaactatt tatcaattcc tgcaattcgt
     1261 ttacaaaacg gcaaatgtga aatccgtcac atactgcgtg atgaacttga attgccaaag
     1321 gaagtataat tttgttatct tctttataat atttccccat agtaaaaata ggaatcaaat
     1381 aatcatatcc tttctgcaaa ttcagattaa agccatcgaa ggttgaccac ggtatcatag
     1441 atacattaaa aatgttttcc ggagcatttg gctttccttc cattctatga ttgtttccat
     1501 accgttgcgt atcactttca taatctgcta aaaatgattt aaagtcagac ttacactcag
     1561 tccaaaggct ggaaaatgtt tcagtatcat tgtgaaatat tgtatagctt ggtatcatct
     1621 catcatatat ccccaattca ccatcttgat tgattgccgt cctaaactct gaatggcggt
     1681 ttacaatcat tgcaatataa taaagcattg caggatatag tttcattccc ttttccttta
     1741 tttgtgtgat atccacttta acggtcatgc tgtaggtaca aggtacactt gcaaagtagt
     1801 ggtcaaaata ctcttttctg ttccaactat ttttatcaat tttttcaaat accatctaag
     1861 ttccctctca aattcaagtt tatcgctcta atgaacaaag atattatacc acatttttgt
     1921 gaatttttca acttgcccac ttcgactgca ctcccgactt aataacttct tgaacacttg
     1981 ccgaaaaaga aaaactgccg ggtacgtacc cgggatcgat ccccgccgag cgctcttccg
     2041 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2101 actcaaaggc ggtaatacgg ttatccactg aatcagggga taacgcagga aagaacatgt
     2161 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2221 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2281 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2341 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2401 cgctttctca atgctcacgc tgtaggtatc acagttcggt gtaggtcgtt cgctccaagc
     2461 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2521 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2581 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2641 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2701 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2761 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2821 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2881 aatgcaagtt tctaactaac atacatcata gttactaaac tatggtaact ataattttat
     2941 taactatatt aagcactgat tagtactata actcaatata agcatatccc ctgtatcgta
     3001 actagagaac caaacgacgg aaaaagcgat atagataagt ttagatagtg caccaatttt
     3061 ttacttttct attttagaat ctagcatttc caatgcttac taatactatt tttgattttt
     3121 aacttgtttt aaaaaagcac ctactaatta ggattctagc tctatattct agttatacat
     3181 tctctatatt ctagctctat attctagtta tacattctct atattctagc tctatattct
     3241 agttatacat tctctatatt ctagctctat attctagtta tacattctct atattctagc
     3301 tctatattct agttatacat tctctatatt ctagctctat attctagtta tacattctct
     3361 atattctagc tctatattct agttatacat tctctaaaaa acatatatat atatactaca
     3421 tatatatgta caattatttt accaaaagaa atttttaaaa cggcttaatt tcaatacttt
     3481 gatgccattt tttttttgca attctcgtac aattatttta ccaaaagaaa tttttattaa
     3541 ttagtttttt tgcataaaaa taggactaga aaatcaattc taatcctatc aaaaatctac
     3601 ttgtttaatc tatatgtata gtacataaat cctctatcat ttattttagt ttcccataca
     3661 tctaaaattt tagtatctaa taaagattct atcatatttg aaattctttg ctgtgttact
     3721 cctaaatctt ttgctaattc ctcttgatta attcttaata tatttccttt tattttatca
     3781 tctttgacca ataaatcttg cttatatctc atatgagtat ataatcttaa ttcttcagtt
     3841 gaaattattc cccaaattac tgccatggta acaaagtaac taatagtata ttgtttatct
     3901 atattacatc taacttcttt tacattgtat gtatctttta ttcctagctt tgtaacacct
     3961 tttgtttttg taatataatc attatgttga agttctttta atgtttctct taatgtcttt
     4021 tcactcataa caatgttttt aactttcttc ttacgtttat aggttatcag ctccattata
     4081 tcgtctatgt ttaattctct atctttattg ttaagtaaca cattatagat aaacaattga
     4141 ttaccagtca ttattttaac ccctttccta ttcttatatt tcaaatcctt tgagtgctta
     4201 tgtggcatat tgaacaaagt atcttcatca ctatatataa aatctgattc tattttattc
     4261 caacaatcac tattaaactc acaacctgta caaccaaaat taaccgtttt aagcttctca
     4321 tacatatact tgacttgata ttctagctca ctatttgaaa gactattttg tgatatatag
     4381 ttccattctc tagcaacgga tattacttga cttaaacttt tattcctcaa tcttaaagtc
     4441 acaactattt tttgcaaatc taggttttta tgtccatctt tactaccatt ttttaaaata
     4501 tctactatac aatttggtag cttactatct aatatatatt ttgtattagt attacctact
     4561 gtttttacat ctttgcaata ttttttagct aacttttcta tgtcatatct atatatattt
     4621 ttgtcttggt gtattatttt tacttgttta gtagtatttt taatattata tgtataaggt
     4681 actcttaata cttgagtaga taaatttgct tgtttatctg cacccaattt atttattaat
     4741 gtatttgtaa cttctgatac taacttaatg ttattagttt tattaattag cacataaaca
     4801 tggaatccat ttccactatc aactaggata tgataatgta atcctatctt agtaaataaa
     4861 ttaattatat ctttataatt aaaaccttgt cctaattctt ttttgtcaaa atcaaatcct
     4921 aatgcatatc tatttattaa gttttcattg gttcctgctt ttccatcagt tgtacatagg
     4981 ttaaaaaatg tattttcata ataaatattt tttgcgaagg taaattctac tatatcatct
     5041 atatttttaa aaaaagttgt tttactatat tctttaaatt cttctccgag ttctaattgt
     5101 ttactattac ttttattatt ttgaaatact ctaacatatt cattatcaat aagattattt
     5161 ccttgtccta taccatctct aaatactgcc attagaaatt ttttcaactt atattttgaa
     5221 tctaattgtt ccatatattt attatctcct tatctagttt ttttaagaga taacactaca
     5281 ttaaatatat acttattgac taatcaactt ttacaactat tgaataattt tagcttagta
     5341 tgtataataa tattgttaag atgctaaaat tgatagcaat attattatat aactacagat
     5401 acttaatcag ttagaccgat attggagttg gtagctccat aaactcaatt ggtagttgag
     5461 ttgtcattgt ctagtagtat tatctcattt tgttgttatc ttaaaatatt attttaaaaa
     5521 tactctgctt ggtcggctta gggtattttt tttgtttaat tccaatattg gttcaaactc
     5581 ctgtgtaata cttttgacac ttccatctgt attgaattga cagttgatat ggtatctcat
     5641 tattggcttg ttcttatgtt tttctatgta tttatttttt actttcaaat aatttgtatt
     5701 tccattttta gttatgttta acacactatc cttattactc aataaaatta ttttttgttt
     5761 taacatatga tttatagaat ctaaaaattg atattgactt ataatagtac tttctacctc
     5821 tctagctttt tctagtgttt caaattcttc aaactctgct atattgctat agtatagctt
     5881 atcattaata atattatctg acaactttaa ttccatttta taaaacaatt ccaagttatg
     5941 tataaaattt ttaatgttac aacatataga cttattattt gttcttatat ggtatctcca
     6001 tttactactt ctagctctca tgttatatgc acaactactc ccaacataaa catatattgc
     6061 ttgttctaac tcatattttt gtaaaatttc aacatcatat ccagtcaaat cgtactctct
     6121 cttatataca aatggttttt ctaatttttt gttcaataaa tctcctccta tttcttactt
     6181 aatttagcta aaaatctatt tatatttcac ctcctttttg actttaagcc tacgaatacc
     6241 atatttatga ttataacatc ttctattttt cccaaatcct tacatctccc ctaattttta
     6301 gacttaaggg cgaattctgc atcaagctag cataaaaata agaagcctgc atttgcaggc
     6361 ttcttatttt tatggtaccc ttattaacgt tgatataatt taaattttat ttgacaaaaa
     6421 tgggctcgtg ttgtacaata aatgtagatc tcctgcagta aaggagaaaa ttttatgaga
     6481 ccaagtaaaa aattattaat agctataata tcaatattct taataagttc agttccagta
     6541 agtgcacatg cagatagtgt ttttacactt gaagattttg tgggtgattg gagacaaact
     6601 gctggatata atttagatca agttttagaa cagggtggag tgagttctct ttttcaaaat
     6661 cttggtgtct cagttacacc aatacaaaga atagttttaa gtggagaaaa tggattaaag
     6721 atcgatatac atgtaataat tccttatgag ggactatcag gggaccaaat gggacaaatt
     6781 gagaaaatat tcaaagttgt atatccagta gacgatcatc actttaaagt aatattacat
     6841 tatggaactt tagtaattga tggtgtaaca ccaaatatga tagattattt tggtagacca
     6901 tacgaaggga ttgcagtttt tgatggaaag aaaataaccg taacaggcac tttgtggaat
     6961 ggaaataaaa taatagatga aaggttaatt aatcctgatg gatctttatt gtttagagta
     7021 actataaacg gtgttacagg ttggagactt tgtgaacgta ttctagcata gg
//
