LOCUS       sequence-168723-       14670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible expression of reprogramming factors (Klf4, Sox2, 
            Oct4,.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Merkl C, Saalfrank A, Riesen N, Kuhn R, Pertek A, Eser S, Hardt MS, 
            Kind A, Saur D, Wurst W, Iglesias A, Schnieke A
  TITLE     Efficient generation of rat induced pluripotent stem cells using a 
            non-viral inducible vector.
  JOURNAL   PLoS One. 2013;8(1):e55170. doi: 10.1371/journal.pone.0055170. Epub 
            2013 Jan 31.
  PUBMED    23383095
REFERENCE   2  (bases 1 to 14670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e55170. doi"; pages: " 
            10.1371/journal.pone.0055170. Epub 2013 Jan 31"
COMMENT     Sequence Label: pREPROII-attB
FEATURES             Location/Qualifiers
     source          1..14670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(114..147)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    199..232
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    complement(269..302)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(316..334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    407..631
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2087..2140)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     intron          3183..3412
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     protein_bind    join(3579..3597,3598..3620,3621..3639,3640..3662,3663..3681,
                     3682..3704,3705..3723,3724..3746,3747..3765,3766..3788,
                     3789..3807,3808..3830,3831..3849)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3882..3920
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4059..4288
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     regulatory      4365..4374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5427..5480
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    6908..7132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        7184..7563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7565..7840
                     /label=chicken beta-actin promoter
     intron          7841..8852
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8776..8798
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8860..8879
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(8941..9558,9559..9564,9565..9600,9601..9603,9604..9639,
                     9640..9642,9643..9678,9679..9687)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(10414..10433)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             10977..10997
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11100..11294
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     polyA_signal    11365..11499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11389..11408)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11443..11462
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(11773..11792)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(11883..11903)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11883..11901)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11922..11938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11922..11938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11935..11957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11946..11962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11970..11976,11977..11994,11995..12000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12015..12036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12153..12170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12324..12912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12404..12423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13083..13874,13875..13943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13706..13725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13944..14048)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14074..14529)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14211..14232)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14423..14442
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(14656..14670,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(14670,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcg
       61 cgatcgcatt accctgttat ccctagtcga caagcgatcg cggccgggtc gacataactt
      121 cgtatagcat acattatacg aagttatccg gccaaggccc gcggggccac tagttctaga
      181 gcgatccccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
      241 ggatcgccac cgcggtggag ctgtcgacat aacttcgtat agcatacatt atacgaagtt
      301 atagctccaa ttcgccctat agtgagtcgt attacgcgcg tcgacattac cctgttatcc
      361 ctaggcgcgc cgtcgacgcc ccagctggtt ctttccgcct cagaagccat agagcccacc
      421 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
      481 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
      541 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
      601 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgtcg agcggccggc
      661 cttaaaagtg cctcttcatg tgtaaggcaa ggtggtccga cctggaaaag gccctgtcgc
      721 acttctggca ctgaaagggc cggtgccctg tgtgtttgcg gtagtgcctg gtcagttcat
      781 ccgagcgggc gaatttccac ccacagccgt cccagtcaca gtggtaaggt ttctcgcctg
      841 tgtgagttcg caggtgtgcc ttgagatgag aactcttggt ataggttttg ccacagcctg
      901 catagtcaca agtgtgggtg gctgttcttt tccggggcca cgaccttctt cccctctttg
      961 gcttgggctc ctctggcagg caggaacccg gtggcatgag ctcttgataa tggagagagg
     1021 ggacttgtga ctgcatctgg tctggcagga aaggagggta gttgggcccc ggatggggat
     1081 ggaatcctgg gggaagaggc aggccagggt gacagtccct gctgttcagc agttcctcgg
     1141 gactcagtgt aggggtagtc ctggtgggga gctgccgccc cagggggaag tcgtgtgtgt
     1201 tgggccggtg gccgttgctg agctggggtc cagcgctcaa atgggcctct agggaccggc
     1261 tgaccgtgca ggacgggacc gcctcttgct taatcttggg gcacatgcgc ggcgggccac
     1321 cgctgtaggg cgccactacc acggggtggc tgccgtctgg gcttcctttg ctaacactga
     1381 tgaccgaagg gctgctgtac tcgctgccag gggtggtcag agacgccttc agcacaaact
     1441 tgcccatcag cccgccacct ggcggctgag gctgctgtgg cggaatgtat actgggtcca
     1501 actccggccg caggagctca gccacgaagc cgcccgaggg gctcacgtca ttgatgtccg
     1561 ccaggttgaa gggggccgtg ggaggtggcg cagattctcg gctgtagagg agccctccac
     1621 ctgtgttgct ggcagccacg cccgggtcac ccccggcccg gatcggatag ctgaagctgc
     1681 aggtggaggg cgcgctggca gggccgctgc tcgctgggga agacgaggat gaagctgacg
     1741 ccgaggtggt cacggtggcg gccaccgatt cctggtgggt tagcgagttg gaaaggataa
     1801 agtctaggtc caggaggtcg ttgaactcct cggtctccct ccgggctagg agggccgggt
     1861 tgttactgct gcaagctgca ccagctccgc cactctccag gtctgtggcc accgtcgccg
     1921 ccaggtcgta ggggcggccg ggaagtgggg gaagtcgctt catgtgagag agttcctcac
     1981 gccaacggtt agtcggggca cctgctggac gcagtgtctt ctcccttccc gccgggccgg
     2041 acgcgaacgt ggagaaggac gggagcagag cgtcgctgac agccataggg ccgggattct
     2101 cctccacgtc accgcatgtt agaagacttc ctctgccctc catgtgcgac aggggcagtg
     2161 tgccgttaat ggccgtgccg ggcaccgggc cgctctggta gtgctgggcc atgtgcagtc
     2221 tactgggcgc agcgggctcc ggcacctcgg cgccggggag gtacatgctg atcatgtccc
     2281 ggaggtcccc ggcctggcag ggcgccctgg agtgggagga agaggtaacc acgggggggc
     2341 tggagctggc ctcggacttg accacagagc ccatggagcc cagcgccata ccgggggtgc
     2401 cctgctgcga gtaggacatg ctgtaggtgg gcgagccgtt catgtaggtc tgcgagctgg
     2461 tcatggagtt gtactgcagg gcgctgacgt cgtagcggtg catcggttgc atctgtgccg
     2521 cgccgtgagc gttgaggccc gggtgctgcg ggtagcccag ctgctcctgc atcatgctgt
     2581 agctgccgtt gctccagccg ttcatgtgcg cgtagctgtc catgcgctgg ttcacgcccg
     2641 cacccaggcc ggcgcccacc ccaaccccgc tcgccatgct gttcccgccg ggggccagca
     2701 agcctccggg aagcgtgtac ttatccttct tcatgagcgt cttggttttc cgccgcggcc
     2761 ggtatttata atccgggtgc tccttcatgt gcagagcgcg cagccgcttg gcctcgtcga
     2821 tgaacggccg cttctcggtc tcggacaaaa gtttccactc cgcgcccagg cgcttgctga
     2881 tctccgagtt gtgcatcttg gggttctcct gggccatctt acgccgctgc ccccgggacc
     2941 ataccatgaa ggcgttcatg ggcctcttga cgcggtccgg gctgttcttc tggttgccgc
     3001 cggtcgccgc cgccgtggcg ttgcctcctc cgccgccgcc ccccgaagct tgctgcgggc
     3061 ccggcggctt cagctccgtc tccatcatgt tatacatcat ggtggcggcg gccggccgcc
     3121 agtgtgatgg atggatatcc gcaggatccg cttgggctgc agttggacct gggagtggac
     3181 acctgtggag agaaaggcaa agtggatgtc attgtcactc aagtgtatgg ccagatctca
     3241 agcctgccac acctcaagct tgacaacaaa aagattgtct tttctgacca gatggacgcg
     3301 gccaccctca aaggcatcac cgcgggccag gtgaatatca aatcctcctc gtttttggaa
     3361 actgacaatc ttagcgcaga agtcatgccc gcttttgaga gggagtactc accccaacag
     3421 ctggccctcg cagacagcga attgtcgacg aattcggggc cgcggaggct ggatcggtcc
     3481 cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
     3541 actaaacgag ctctgcttat ataggtcgag tttaccactc cctatcagtg atagagaaaa
     3601 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     3661 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     3721 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     3781 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     3841 tgatagagaa aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg
     3901 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     3961 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cccgaattct
     4021 taattaaaat tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc
     4081 gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac
     4141 ctggcccgcg gtgaggcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt
     4201 tttgttgtca agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat
     4261 gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact gcagcccaag
     4321 cggatcctgc ggatatccat ccatcacact ggcggccggc cgccgccacc atggctggac
     4381 acctggcttc agacttcgcc ttctcacccc caccaggtgg gggtgatggg tcagcagggc
     4441 tggagccggg ctgggtggat cctcgaacct ggctaagctt ccaagggcct ccaggtgggc
     4501 ctggaatcgg accaggctca gaggtattgg ggatctcccc atgtccgccc gcatacgagt
     4561 tctgcggagg gatggcatac tgtggacctc aggttggact gggcctagtc ccccaagttg
     4621 gcgtggagac tttgcagcct gagggccagg caggagcacg agtggaaagc aactcagagg
     4681 gaacctcctc tgagccctgt gccgaccgcc ccaatgccgt gaagttggag aaggtggaac
     4741 caactcccga ggagtcccag gacatgaaag ccctgcagaa ggagctagaa cagtttgcca
     4801 agctgctgaa gcagaagagg atcaccttgg ggtacaccca ggccgacgtg gggctcaccc
     4861 tgggcgttct ctttggaaag gtgttcagcc agaccaccat ctgtcgcttc gaggccttgc
     4921 agctcagcct taagaacatg tgtaagctgc ggcccctgct ggagaagtgg gtggaggaag
     4981 ccgacaacaa tgagaacctt caggagatat gcaaatcgga gaccctggtg caggcccgga
     5041 agagaaagcg aactagcatt gagaaccgtg tgaggtggag tctggagacc atgtttctga
     5101 agtgcccgaa gccctcccta cagcagatca ctcacatcgc caatcagctt gggctagaga
     5161 aggatgtggt tcgagtatgg ttctgtaacc ggcgccagaa gggcaaaaga tcaagtattg
     5221 agtattccca acgagaagag tatgaggcta cagggacacc tttcccaggg ggggctgtat
     5281 cctttcctct gcccccaggt ccccactttg gcaccccagg ctatggaagc ccccacttca
     5341 ccacactcta ctcagtccct tttcctgagg gcgaggcctt tccctctgtt cccgtcactg
     5401 ctctgggctc tcccatgcat tcaaacgagg gcagaggaag tcttctaaca tgcggtgacg
     5461 tggaggagaa tcccggccct atgcccctca acgtgaactt caccaacagg aactatgacc
     5521 tcgactacga ctccgtacag ccctatttca tctgcgacga ggaagagaat ttctatcacc
     5581 agcaacagca gagcgagctg cagccgcccg cgcccagtga ggatatctgg aagaaattcg
     5641 agctgcttcc caccccgccc ctgtccccga gccgccgctc cgggctctgc tctccatcct
     5701 atgttgcggt cgctacgtcc ttctccccaa gggaagacga tgacggcggc ggtggcaact
     5761 tctccaccgc cgatcagctg gagatgatga ccgagttact tggaggagac atggtgaacc
     5821 agagcttcat ctgcgatcct gacgacgaga ccttcatcaa gaacatcatc atccaggact
     5881 gtatgtggag cggtttctca gccgctgcca agctggtctc ggagaagctg gcctcctacc
     5941 aggctgcgcg caaagacagc accagcctga gccccgcccg cgggcacagc gtctgctcca
     6001 cctccagcct gtacctgcag gacctcaccg ccgccgcgtc cgagtgcatt gacccctcag
     6061 tggtctttcc ctacccgctc aacgacagca gctcgcccaa atcctgtacc tcgtccgatt
     6121 ccacggcctt ctctccttcc tcggactcgc tgctgtcctc cgagtcctcc ccacgggcca
     6181 gccctgagcc cctagtgctg catgaggaga caccgcccac caccagcagc gactctgaag
     6241 aagagcaaga agatgaggaa gaaattgatg tggtgtctgt ggagaagagg caaacccctg
     6301 ccaagaggtc ggagtcgggc tcatctccat cccgaggcca cagcaaacct ccgcacagcc
     6361 cactggtcct caagaggtgc cacgtctcca ctcaccagca caactacgcc gcacccccct
     6421 ccacaaggaa ggactatcca gctgccaaga gggccaagtt ggacagtggc agggtcctga
     6481 agcagatcag caacaaccgc aagtgctcca gccccaggtc ctcagacacg gaggaaaacg
     6541 acaagaggcg gacacacaac gtcttggaac gtcagaggag gaacgagctg aagcgcagct
     6601 tttttgccct gcgtgaccag atccctgaat tggaaaacaa cgaaaaggcc cccaaggtag
     6661 tgatcctcaa aaaagccacc gcctacatcc tgtccattca agcagacgag cacaagctca
     6721 cctctgaaaa ggacttattg aggaaacgac gagaacagtt gaaacacaaa ctcgaacagc
     6781 ttcgaaactc tggtgcataa taaacgtcga gcagcacgag gtggctgtgg ccagatactg
     6841 cgacctccct agcaaactgg ggcacaaact taattgaggc cggccgctcg acgctgatca
     6901 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6961 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7021 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7081 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7141 gcggaaagaa ccagctgggg cttaattaag gcgcgccgga ttcgacattg attattgact
     7201 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7261 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7321 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7381 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7441 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7501 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7561 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     7621 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
     7681 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     7741 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     7801 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc
     7861 gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     7921 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     7981 ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg
     8041 ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     8101 ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     8161 tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     8221 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     8281 tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac
     8341 ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc
     8401 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     8461 tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     8521 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     8581 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     8641 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     8701 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     8761 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     8821 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     8881 tgtctcatca ttttggcaaa gaattcgtcg acgggatcct ggccagctag cgaattcacc
     8941 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc
     9001 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     9061 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     9121 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     9181 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     9241 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     9301 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     9361 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     9421 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     9481 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     9541 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     9601 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     9661 tttgaccttg acatgctccc cgggtaacta ttaaaacagc tctggggttg tacccacccc
     9721 agaggcccac gtggcggcta gtactccggt attgcggtac ccttgtacgc ctgttttata
     9781 ctcccttccc gtaacttaga cgcacaaaac caagttcaat agaagggggt acaaaccagt
     9841 accaccacga acaagcactt ctgtttcccc ggtgatgtcg tatagactgc ttgcgtggtt
     9901 gaaagcgacg gatccgttat ccgcttatgt acttcgagaa gcccagtacc acctcggaat
     9961 cttcgatgcg ttgcgctcag cactcaaccc cagagtgtag cttaggctga tgagtctgga
    10021 catccctcac cggtgacggt ggtccaggct gcgttggcgg cctacctatg gctaacgcca
    10081 tgggacgcta gttgtgaaca aggtgtgaag agcctattga gctacataag aatcctccgg
    10141 cccctgaatg cggctaatcc caacctcgga gcaggtggtc acaaaccagt gattggcctg
    10201 tcgtaacgcg caagtccgtg gcggaaccga ctactttggg tgtccgtgtt tccttttatt
    10261 ttattgtggc tgcttatggt gacaatcaca gattgttatc ataaagcgaa ttggattccc
    10321 gggtaactaa gtaaggatcc atgtctagat tagataaaag taaagtgatt aacagcgcat
    10381 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
    10441 ttggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggcc cttatgaaca
    10501 tgctttcaga ggcaatactg gcgaagcatc acacccgttc agcaccgtta ccgactgaga
    10561 gttggcagca gtttctccag gaaaatgctc tgagtttccg taaagcatta ctggtccatc
    10621 gtgatggagc ccgattgcat atagggacct ctcctacgcc cccccagttt gaacaagcag
    10681 aggcgcaact acgctgtcta tgcgatgcag ggttttcggt cgaggaggct cttttcattc
    10741 tgcaatctat cagccatttt acgttgggtg cagtattaga ggagcaagca acaaaccaga
    10801 tagaaaataa tcatgtgata gacgctgcac caccattatt acaagaggca tttaatattc
    10861 aggcgagaac ctctgctgaa atggccttcc atttcgggct gaaatcatta atatttggat
    10921 tttctgcaca gttagatgaa aaaaagcata cacccattga ggatggtaat aaatcgccaa
    10981 aaaagaagag aaaggtcgac ggcggtggtg ctttgtctcc tcagcactct gctgtcactc
    11041 aaggaagtat catcaagaac aaggagggca tggatgctaa gtcactaact gcctggtccc
    11101 ggacactggt gaccttcaag gatgtatttg tggacttcac cagggaggag tggaagctgc
    11161 tggacactgc tcagcagatc gtgtacagaa atgtgatgct ggagaactat aagaacctgg
    11221 tttccttggg ttatcagctt actaagccag atgtgatcct ccggttggag aagggagaag
    11281 agccctggct ggtggagaga gaaattcacc aagagaccca tcctgattca gagactgcat
    11341 ttgaaatcaa atcatcagtt taaagatcca gacatgataa gatacattga tgagtttgga
    11401 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
    11461 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
    11521 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
    11581 aaatgtggta tggctgatta tgatcctgca agcctcgtcg tcctggccgg accacgctat
    11641 ctgtgcaagg tccccggccc cggacgcgcg agcttggcga gattttcagg agctaaggaa
    11701 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
    11761 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
    11821 ttgcggccgc taactataac ggtcctaagg tagcgagcga tcgcggtacc cagcttttgt
    11881 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
    11941 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12001 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12061 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12121 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    12181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    12241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    12301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    12361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    12421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    12481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    12601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    12781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    12841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    12901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    12961 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    13021 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    13081 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    13141 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    13201 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    13261 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    13321 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    13381 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    13441 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    13501 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13561 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    13621 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13681 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    13741 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    13801 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    13861 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    13921 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    13981 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    14041 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
    14101 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
    14161 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
    14221 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
    14281 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
    14341 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    14401 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    14461 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    14521 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    14581 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    14641 taacgccagg gttttcccag tcacgacgtt
//
