LOCUS       sequence-168725-        4098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FBXO15 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FBXO15_WT
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1498..1597)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1614..1633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1615..1633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1638..1654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1638..1654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2085..2876
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2969..3557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3458..3477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3711..3728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3887..3914)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4006..4092)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccttca gaaatcttgc tgaagatatt ttcctacttg gatgctgtga
      241 gccttctgtg tactggatgt gtgagcaggc gcttttatca tctagccaat gacaatttta
      301 tttggatcgg aatctactca actgcttttt cacctgcaag atcaaattgg aaatttaatt
      361 cagtagagaa gatagctatg tctatgagct ttctgtcagt tcaggataaa gaagctggtt
      421 attggaagaa agaatatatc acaaaacaaa tagcatctgt aaaagccgca ctagctgaca
      481 ttctcaaacc tgtcaaccct tacacaggcc ttccagttaa gaccaaagag gccctcagaa
      541 tatttggttt aggttgggca attatactga aagaaaaagg tggaaaagaa tatatcatgg
      601 agcatgttga tctttccata aatgacacat cagttactgt tatatggtat ggcaaaaaat
      661 ggccatgcct agcatcattg tcaaccttag atttatgtgg catgacacca gtttttaccg
      721 actggtataa aactcccacc aaacatagac tccgatggca ttctttaatt gcaaagtaca
      781 atctgagtca tttgaccata tctaccatga ttggctgtga cagactcatt cggatcttct
      841 gcctgcaccc tggcctcctg gtgggagtgt ggaagaagga ggaagaactg gcttttgtta
      901 tggcaaatct tcattttcat caccttgtgg agaggagcac attaggctcg gctactatcc
      961 cctatgaact gcctccacat agcccctttt tggatgatag ccccgagtat ggactgcacg
     1021 gctaccaact ccatgttgat ctgcacagcg gtggggtttt ctacctatgt ggtacatttc
     1081 gcaatctctt caccaagaga ggaaatattg aaaatggaca tgtgaagctc attgttatac
     1141 atttaaaaaa taacagagaa cacctacctc ttattggaaa agttggcctc tcgtggaaaa
     1201 ctgatatttt tgatggctgt ataaagagtt gttccatgat ggacgtaact cttttggatg
     1261 aacatgggaa acccttttgg tgtttcagtt ccccggtgtg cctgagatcg cctgccacac
     1321 cctctgacag ctctagcttc ttgggacaga catacaacgt ggactacgtt gatgcggaag
     1381 gaagagtgca cgtggagctg gtgtggatca gagagaccga agaatacctt attgtcaacc
     1441 tggtccttta tcttagtatc gcaaaaatca accattggtt tgggacagaa tattgattgc
     1501 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1561 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1621 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1681 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     1741 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1801 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1861 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1921 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1981 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2041 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2101 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2161 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2221 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2281 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2341 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2401 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2461 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2521 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2581 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2641 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2701 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2761 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2821 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2881 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3721 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3781 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3841 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3901 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3961 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4021 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4081 tcgttttatt tgatgcct
//
