LOCUS       sequence-168726-        4335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACVR2A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ACVR2A_WT
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1735..1834)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1851..1870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1852..1870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1875..1891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1875..1891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2322..3113
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3206..3794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3695..3714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3948..3965
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4124..4151)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4243..4329)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgggagct gctgcaaagt tggcgtttgc cgtctttctt atctcctgtt
      241 cttcaggtgc tatacttggt agatcagaaa ctcaggagtg tcttttcttt aatgctaatt
      301 gggaaaaaga cagaaccaat caaactggtg ttgaaccgtg ttatggtgac aaagataaac
      361 ggcggcattg ttttgctacc tggaagaata tttctggttc cattgaaata gtgaaacaag
      421 gttgttggct ggatgatatc aactgctatg acaggactga ttgtgtagaa aaaaaagaca
      481 gccctgaagt atatttttgt tgctgtgagg gcaatatgtg taatgaaaag ttttcttatt
      541 ttccagagat ggaagtcaca cagcccactt caaatccagt tacacctaag ccaccctatt
      601 acaacatcct gctctattcc ttggtgccac ttatgttaat tgcggggatt gtcatttgtg
      661 cattttgggt gtacaggcat cacaagatgg cctaccctcc tgtacttgtt ccaactcaag
      721 acccaggacc acccccacct tctccattac taggtttgaa accactgcag ttattagaag
      781 tgaaagcaag gggaagattt ggttgtgtct ggaaagccca gttgcttaac gaatatgtgg
      841 ctgtcaaaat atttccaata caggacaaac agtcatggca aaatgaatac gaagtctaca
      901 gtttgcctgg aatgaagcat gagaacatat tacagttcat tggtgcagaa aaacgaggca
      961 ccagtgttga tgtggatctt tggctgatca cagcatttca tgaaaagggt tcactatcag
     1021 actttcttaa ggctaatgtg gtctcttgga atgaactgtg tcatattgca gaaaccatgg
     1081 ctagaggatt ggcatattta catgaggata tacctggcct aaaagatggc cacaaacctg
     1141 ccatatctca cagggacatc aaaagtaaaa atgtgctgtt gaaaaacaac ctgacagctt
     1201 gcattgctga ctttgggttg gccttaaaat ttgaggctgg caagtctgca ggcgataccc
     1261 atggacaggt tggtacccgg aggtacatgg ctccagaggt attagagggt gctataaact
     1321 tccaaaggga tgcatttttg aggatagata tgtatgccat gggattagtc ctatgggaac
     1381 tggcttctcg ctgtactgct gcagatggac ctgtagatga atacatgttg ccatttgagg
     1441 aggaaattgg ccagcatcca tctcttgaag acatgcagga agttgttgtg cataaaaaaa
     1501 agaggcctgt tttaagagat tattggcaga aacatgctgg aatggcaatg ctctgtgaaa
     1561 ccattgaaga atgttgggat cacgacgcag aagccaggtt atcagctgga tgtgtaggtg
     1621 aaagaattac ccagatgcag agactaacaa atattattac cacagaggac attgtaacag
     1681 tggtcacaat ggtgacaaat gttgactttc ctcccaaaga atctagtcta tgattgccaa
     1741 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1801 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1861 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1921 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     1981 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2041 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2101 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2161 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2221 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2281 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2341 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2401 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2461 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2521 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2581 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2641 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2701 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2761 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2821 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2881 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2941 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3001 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3061 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3121 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3181 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3241 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3301 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3361 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3421 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3481 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3541 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3601 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3661 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3721 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3781 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3841 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3901 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3961 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4021 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4081 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4141 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4201 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4261 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4321 ttttatttga tgcct
//
