LOCUS       sequence-168728-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA protease chain with V1-MESA ectodomain, 45 extracellular 
            linkers, a flag tag, and Tev protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V1-MESA-45F-Tev
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgcca ccatggcttg gaccagctta attcttagct
      121 tgcttgccct gtgcagcggt gcatcatctg acatacagat gactcagtca cctagcagtc
      181 tgtctgcttc agtcggtgat agggtaacta tcacctgtcg ggcatctcag gatgtgtcca
      241 cggcagttgc ctggtaccag cagaagcccg gcaaggcgcc taagttgctt atctattcag
      301 cttcattttt gtatagtggg gtgcctagcc ggtttagcgg aagtggttct ggcacagact
      361 tcactttgac aatcagttcc ctccaaccgg aggatttcgc tacctactac tgtcagcagg
      421 ggtacggaaa cccctttacg tttggacagg gcactaaagt ggagattaag aggggaggcg
      481 gaggctccgg tgggggaggc tctggtggtg gaggtagcga ggttcagctg gtggaaagtg
      541 gtggcggact agtacagcca ggcggctccc tgcggctgag ttgcgccgcg agtggcttta
      601 ctatatctga ctattggatc cattgggtca ggcaagcccc tgggaaagga ttggagtggg
      661 ttgctggtat tacccccgca ggtgggtata cttattatgc agatagcgtg aaagggaggt
      721 tcaccatctc tgcagacaca agcaagaaca cggcctacct acagatgaac tctctgaggg
      781 cagaggacac tgccgtctac tattgcgccc gatttgtgtt ttttctaccc tacgccatgg
      841 actattgggg acaggggaca ttggtaggcg ccgggacagg agggtctggg ggtggcactg
      901 gcggatctgg aggagactac aaggacgatg acgacaacgg cggatcgggg gggtctggtg
      961 gtagtggcgg ttcgggggga ggtaccggtc tggtcgt
//
