LOCUS       sequence-168730-        6102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CG , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CG_WT
FEATURES             Location/Qualifiers
     source          1..6102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3098..3109
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3502..3601)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3618..3637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3619..3637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3642..3658)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3642..3658)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4089..4880
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4973..5561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5462..5481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5715..5732
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5891..5918)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6010..6096)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggggctg gagaactata aacagcccgt ggtgctgaga gaggacaact
      241 gccgaaggcg ccggaggatg aagccgcgca gtgctgcggc cagcctgtcc tccatggagc
      301 tcatccccat cgagttcgtg ctgcccacca gccagcgcaa atgcaagagc cccgaaacgg
      361 cgctgctgca cgtggccggc cacggcaacg tggagcagat gaaggcccag gtgtggctgc
      421 gagcgctgga gaccagcgtg gcggcggact tctaccaccg gctgggaccg catcacttcc
      481 tcctgctcta tcagaagaag gggcagtggt acgagatcta cgacaagtac caggtggtgc
      541 agactctgga ctgcctgcgc tactggaagg ccacgcaccg gagcccgggc cagatccacc
      601 tggtgcagcg gcacccgccc tccgaggagt cccaagcctt ccagcggcag ctcacggcgc
      661 tgattggcta tgacgtcact gacgtcagca acgtgcacga cgatgagctg gagttcacgc
      721 gccgtggctt ggtgaccccg cgcatggcgg aggtggccag ccgcgacccc aagctctacg
      781 ccatgcaccc gtgggtgacg tccaagcccc tcccggagta cctgtggaag aagattgcca
      841 acaactgcat cttcatcgtc attcaccgca gcaccaccag ccagaccatt aaggtctcac
      901 ccgacgacac ccccggcgcc atcctgcaga gcttcttcac caagatggcc aagaagaaat
      961 ctctgatgga tattcccgaa agccaaagcg aacaggattt tgtgctgcgc gtctgtggcc
     1021 gggatgagta cctggtgggc gaaacgccca tcaaaaactt ccagtgggtg aggcactgcc
     1081 tcaagaacgg agaagagatt cacgtggtac tggacacgcc tccagacccg gccctagacg
     1141 aggtgaggaa ggaagagtgg ccgctggtgg acgactgcac gggagtcacc ggctaccatg
     1201 agcagcttac catccacggc aaggaccacg agagtgtgtt caccgtgtcc ctgtgggact
     1261 gcgaccgcaa gttcagggtc aagatcagag gcattgatat ccccgtcctg cctcggaaca
     1321 ccgacctcac agtttttgta gaggcaaaca tccagcatgg gcaacaagtc ctttgccaaa
     1381 ggagaaccag ccccaaaccc ttcacagagg aggtgctgtg gaatgtgtgg cttgagttca
     1441 gtatcaaaat caaagacttg cccaaagggg ctctactgaa cctccagatc tactgcggta
     1501 aagctccagc actgtccagc aaggcctctg cagagtcccc cagttctgag tccaagggca
     1561 aagttcagct tctctattat gtgaacctgc tgctgataga ccaccgtttc ctcctgcgcc
     1621 gtggagaata cgtcctccac atgtggcaga tatctgggaa gggagaagac caaggaagct
     1681 tcaatgctga caaactcacg tctgcaacta acccagacaa ggagaactca atgtccatct
     1741 ccattcttct ggacaattac tgccacccga tagccctgcc taagcatcag cccacccctg
     1801 acccggaagg ggaccgggtt cgagcagaaa tgcccaacca gcttcgcaag caattggagg
     1861 cgatcatagc cactgatcca cttaaccctc tcacagcaga ggacaaagaa ttgctctggc
     1921 attttagata cgaaagcctt aagcacccaa aagcatatcc taagctattt agttcagtga
     1981 aatggggaca gcaagaaatt gtggccaaaa cataccaatt gttggccaga agggaagtct
     2041 gggatcaaag tgctttggat gttgggttaa caatgcagct cctggactgc aacttctcag
     2101 atgaaaatgt aagagccatt gcagttcaga aactggagag cttggaggac gatgatgttc
     2161 tgcattacct tctacaattg gtccaggctg tgaaatttga accataccat gatagtgccc
     2221 ttgccagatt tctgctgaag cgtggtttaa gaaacaaaag aattggtcac tttttgtttt
     2281 ggttcttgag aagtgagata gcccagtcca gacactatca gcagaggttc gctgtgattc
     2341 tggaagccta tctgaggggc tgtggcacag ccatgctgca cgactttacc caacaagtcc
     2401 aagtaatcga gatgttacaa aaagtcaccc ttgatattaa atcgctctct gctgaaaagt
     2461 atgacgtcag ttcccaagtt atttcacaac ttaaacaaaa gcttgaaaac ctgcagaatt
     2521 ctcaactccc cgaaagcttt agagttccat atgatcctgg actgaaagca ggagcgctgg
     2581 caattgaaaa atgtaaagta atggcctcca agaaaaaacc actatggctt gagtttaaat
     2641 gtgccgatcc tacagcccta tcaaatgaaa caattggaat tatctttaaa catggtgatg
     2701 atctgcgcca agacatgctt attttacaga ttctacgaat catggagtct atttgggaga
     2761 ctgaatcttt ggatctatgc ctcctgccat atggttgcat ttcaactggt gacaaaatag
     2821 gaatgatcga gattgtgaaa gacgccacga caattgccaa aattcagcaa agcacagtgg
     2881 gcaacacggg agcatttaaa gatgaagtcc tgaatcactg gctcaaagaa aaatccccta
     2941 ctgaagaaaa gtttcaggca gcagtggaga gatttgttta ttcctgtgca ggctactgtg
     3001 tggcaacctt tgttcttgga ataggcgaca gacacaatga caatattatg atcaccgaga
     3061 caggaaacct atttcatatt gacttcgggc acattcttgg gaattacaaa agtttcctgg
     3121 gcattaataa agagagagtg ccatttgtgc taacccctga cttcctcttt gtgatgggaa
     3181 cttctggaaa gaagacaagc ccacacttcc agaaatttca ggacatctgt gttaaggctt
     3241 atctagccct tcgtcatcac acaaacctac tgatcatcct gttctccatg atgctgatga
     3301 caggaatgcc ccagttaaca agcaaagaag acattgaata tatccgggat gccctcacag
     3361 tggggaaaaa tgaggaggat gctaaaaagt attttcttga tcagatcgaa gtttgcagag
     3421 acaaaggatg gactgtgcag tttaattggt ttctacatct tgttcttggc atcaaacaag
     3481 gagagaaaca ttcagcctga ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     3541 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     3601 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     3661 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     3721 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     3781 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     3841 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     3901 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     3961 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     4021 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     4081 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     4141 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     4201 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     4261 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     4321 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     4381 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     4441 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     4501 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     4561 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     4621 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     4681 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     4741 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     4801 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     4861 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     4921 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4981 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5101 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5221 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5281 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5341 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5401 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5461 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5521 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5581 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5641 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5701 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5761 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5821 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     5881 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     5941 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     6001 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     6061 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
