LOCUS       sequence-168731-        6420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes blinded measles virus H glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther Methods."; date: "2012-04"; volume: "23(2)"; pages: "137-47. 
            Epub 2012 May 21"
COMMENT     Sequence Label: pCG-Hc∆18-AA
FEATURES             Location/Qualifiers
     source          1..6420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        321..524
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     475..495
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     521..545
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          644..1216
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1224..1243
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1271..1289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3142..3159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3358..3377)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3423..3478
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3477..3496)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3825..3960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4069..4086)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4240..4828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4320..4339)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4999..5790,5791..5859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5622..5641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5860..5964)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6032..6050
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6099..6121)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6256..6275
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaattcgagc tcgccccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
       61 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      121 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      181 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      241 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      301 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      361 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      421 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      481 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      541 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      601 ccagcctccg ggggatcgat cccccgatcc tgagaacttc agggtgagtt tggggaccct
      661 tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg gcaaagtttt
      721 cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct catgataatt
      781 ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt tcttttcatt
      841 ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta aattcacttt
      901 tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag gcaatcaggg
      961 tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta aatgataagg
     1021 tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg gtagaaacaa
     1081 ctacatcctg gtcatcatcc tgcctttctc tttatggtta caatgatata cactgtttga
     1141 gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg ttcatgcctt
     1201 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     1261 caaagaattg taatacgact cactataggg cgaattcgga tcccccggta gttaattaaa
     1321 aattagggtg caagatcatc gataatgggc agccggatcg tgatcaaccg ggagcacctg
     1381 atgatcgacc ggccctacgt gctgctggcc gtgctgttcg tgatgttcct gagcctgatc
     1441 ggcttgctag ccattgctgg aatccggctg cacagagccg ccatctacac cgccgagatc
     1501 cacaagagcc tgagcaccaa cctggacgtg accaacagca tcgagcatca ggtcaaggac
     1561 gtgctgaccc ccctgtttaa gatcatcggc gacgaagtgg gcctgcggac cccccagaga
     1621 ttcaccgacc tggtcaagtt catcagcgac aagatcaagt tcctgaaccc cgaccgggag
     1681 tacgacttcc gggacctgac ctggtgcatc aacccccccg agcggatcaa gctggactac
     1741 gaccagtact gcgccgatgt ggccgccgag gaactgatga atgcattggt gaactcaact
     1801 ctactggaga ccagaacaac caatcagttc ctagctgtct caaagggaaa ctgctcaggg
     1861 cccactacaa tcagaggtca attctcaaac atgtcgctgt ccctgttaga cttgtattta
     1921 ggtcgaggtt acaatgtgtc atctatagtc actatgacat cccagggaat gtatggggga
     1981 acttacctag tggaaaagcc taatctgagc agcaaaaggt cagagttgtc acaactgagc
     2041 atgtaccgag tgtttgaagt aggtgttatc agaaatccgg gtttgggggc tccggtgttc
     2101 catatgacaa actatcttga gcaaccagtc agtaatgatc tcagcaactg tatggtggct
     2161 ttgggggagc tcaaactcgc agccctttgt cacggggaag attctatcac aattccctat
     2221 cagggatcag ggaaaggtgt cagcttccag ctcgtcaagc taggtgtctg gaaatcccca
     2281 accgacatgc aatcctgggt ccccttatca acggatgatc cagtgataga caggctttac
     2341 ctctcatctc acagaggtgt tatcgctgac aaccaagcaa aatgggctgt cccgacaaca
     2401 cgaacagatg acaagttgcg aatggagaca tgcttccaac aggcgtgtaa gggtaaaatc
     2461 caagcactct gcgagaatcc cgagtgggca ccattgaagg ataacaggat tccttcatac
     2521 ggggtcttgt ctgttgatct gagtctgaca gttgagctta aaatcaaaat tgcttcggga
     2581 ttcgggccat tgatcacaca cggttcaggg atggacctat acaaatccaa ccacaacaat
     2641 gtgtattggc tgactatccc gccaatgaag aacctagcct taggtgtaat caacacattg
     2701 gagtggatac cgagattcaa ggttagtccc gcgctcttca atgtcccaat taaggaagca
     2761 ggcggagact gccatgcccc aacataccta cctgcggagg tggatggtga tgtcaaactc
     2821 agttccaatc tggtgattct acctggtcaa gatctccaat atgttttggc aacctacgat
     2881 acttccgcgg ttgaacatgc tgtggtttat tacgtttaca gcccaagccg ctcattttct
     2941 tacttttatc cttttaggtt gcctataaag ggggtcccca tcgaattaca agtggaatgc
     3001 ttcacatggg accaaaaact ctggtgccgt cacttctgtg tgcttgcgga ctcagaatct
     3061 ggtggacata tcactcactc tgggatggtg ggcatgggag tcagctgcac agtcacccgg
     3121 gaagcggccg caagaggatc gcatcaccat caccatcact gatagtttgt actagtgtga
     3181 aatagacatc agaattaaga aaaacgtagg gtccaagtgg ttccccgtta tggactcgct
     3241 atctgtcaac cagatcttat accctgaagt tcacctagat agcccgatag ttaccaataa
     3301 gatagtagcc atcctggagt atgctcgact gtttaaacct gcaggcatgc aagctgatct
     3361 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     3421 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     3481 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     3541 tttggcaaca tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc
     3601 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     3661 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     3721 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     3781 tccctcttct cttatgaact cgaggagctt tttgcaaaag cctaggcctc caaaaaagcc
     3841 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     3901 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     3961 gggcggagtt aggggcggga ctatggttgc tgactaattg agactgcatt aatgaatcgg
     4021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     4441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4681 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5821 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6001 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     6061 tcaagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6121 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     6181 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     6241 gttggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atcgacgctc
     6301 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     6361 cgccgccgca aggaatggtg ctggcttatc gaaattaatc gactcactat agggagaccc
//
