LOCUS       sequence-168743-        6003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CB_p.A1048V
FEATURES             Location/Qualifiers
     source          1..6003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             879..887
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2186..2197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3882..3981)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3998..4017)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3999..4017)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4022..4038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4022..4038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4469..5260
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5353..5941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5842..5861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtgcttca gtttcataat gcctcctgct atggcagaca tccttgacat
      721 ctgggcggtg gattcacaga tagcatctga tggctccata cctgtggatt tccttttgcc
      781 cactgggatt tatatccagt tggaggtacc tcgggaagct accatttctt atattaagca
      841 gatgttatgg aagcaagttc acaattaccc aatgttcaac ctccttatgg atattgactc
      901 ctatatgttt gcatgtgtga atcagactgc tgtatatgag gagcttgaag atgaaacacg
      961 aagactctgt gatgtcagac cttttcttcc agttctcaaa ttagtgacaa gaagttgtga
     1021 cccaggggaa aaattagact caaaaattgg agtccttata ggaaaaggtc tgcatgaatt
     1081 tgattccttg aaggatcctg aagtaaatga atttcgaaga aaaatgcgca aattcagcga
     1141 ggaaaaaatc ctgtcacttg tgggattgtc ttggatggac tggctaaaac aaacatatcc
     1201 accagagcat gaaccatcca tccctgaaaa cttagaagat aaactttatg ggggaaagct
     1261 catcgtagct gttcattttg aaaactgcca ggacgtgttt agctttcaag tgtctcctaa
     1321 tatgaatcct atcaaagtaa atgaattggc aatccaaaaa cgtttgacta ttcatgggaa
     1381 ggaagatgaa gttagcccct atgattatgt gttgcaagtc agcgggagag tagaatatgt
     1441 ttttggtgat catccactaa ttcagttcca gtatatccgg aactgtgtga tgaacagagc
     1501 cctgccccat tttatacttg tggaatgctg caagatcaag aaaatgtatg aacaagaaat
     1561 gattgccata gaggctgcca taaatcgaaa ttcatctaat cttcctcttc cattaccacc
     1621 aaagaaaaca cgaattattt ctcatgtttg ggaaaataac aaccctttcc aaattgtctt
     1681 ggttaaggga aataaactta acacagagga aactgtaaaa gttcatgtca gggctggtct
     1741 ttttcatggt actgagctcc tgtgtaaaac catcgtaagc tcagaggtat cagggaaaaa
     1801 tgatcatatt tggaatgaac cactggaatt tgatattaat atttgtgact taccaagaat
     1861 ggctcgatta tgttttgctg tttatgcagt tttggataaa gtaaaaacga agaaatcaac
     1921 gaaaactatt aatccctcta aatatcagac catcaggaaa gctggaaaag tgcattatcc
     1981 tgtagcgtgg gtaaatacga tggtttttga ctttaaagga caattgagaa ctggagacat
     2041 aatattacac agctggtctt catttcctga tgaactcgaa gaaatgttga atccaatggg
     2101 aactgttcaa acaaatccat atactgaaaa tgcaacagct ttgcatgtta aatttccaga
     2161 gaataaaaaa caaccttatt attaccctcc cttcgataag attattgaaa aggcagctga
     2221 gattgcaagc agtgatagtg ctaatgtgtc aagtcgaggt ggaaaaaagt ttcttcctgt
     2281 attgaaagaa atcttggaca gggatccctt gtctcaactg tgtgaaaatg aaatggatct
     2341 tatttggact ttgcgacaag actgccgaga gattttccca caatcactgc caaaattact
     2401 gctgtcaatc aagtggaata aacttgagga tgttgctcag cttcaggcgc tgcttcagat
     2461 ttggcctaaa ctgccccccc gggaggccct agagcttctg gatttcaact atccagacca
     2521 gtacgttcga gaatatgctg taggctgcct gcgacagatg agtgatgaag aactttctca
     2581 atatctttta caactggtgc aagtgttaaa atatgagcct tttcttgatt gtgccctctc
     2641 tagattccta ttagaaagag cacttggtaa tcggaggata gggcagtttc tattttggca
     2701 tcttaggtca gaagtgcaca ttcctgctgt ctcagtacaa tttggtgtca tccttgaagc
     2761 atactgccgg ggaagtgtgg ggcacatgaa agtgctttct aagcaggttg aagcactcaa
     2821 taagttaaaa actttaaata gtttaatcaa actgaatgcc gtgaagttaa acagagccaa
     2881 agggaaggag gccatgcata cctgtttaaa acagagtgct taccgggaag ccctctctga
     2941 cctgcagtca cccctgaacc catgtgttat cctctcagaa ctctatgttg aaaagtgcaa
     3001 atacatggat tccaaaatga agcctttgtg gctggtatac aataacaagg tatttggtga
     3061 ggattcagtt ggagtgattt ttaaaaatgg tgatgattta cgacaggata tgttgacact
     3121 ccaaatgttg cgcttgatgg atttactctg gaaagaagct ggtttggatc ttcggatgtt
     3181 gccttatggc tgtttagcaa caggagatcg ctctggcctc attgaagttg tgagcacctc
     3241 tgaaacaatt gctgacattc agctgaacag tagcaatgtg gctgctgcag cagccttcaa
     3301 caaagatgcc cttctgaact ggcttaaaga atacaactct ggggatgacc tggaccgagc
     3361 cattgaggaa tttacactgt cctgtgctgg ctactgtgta gcttcttatg tccttgggat
     3421 tggtgacaga catagtgaca acatcatggt caaaaaaact ggccagctct tccacattga
     3481 ctttggacat attcttggaa atttcaaatc taagtttggc attaaaaggg agcgagtgcc
     3541 ttttattctt acctatgatt tcatccatgt cattcaacaa ggaaaaacag gaaatacaga
     3601 aaagtttggc cggttccgcc agtgttgtga ggatgcatat ctgattttac gacggcatgg
     3661 gaatctcttc atcactctct ttgcgctgat gttgactgca gggcttcctg aactcacatc
     3721 agtcaaagat atacagtatc ttaaggactc tcttgcatta gggaagagtg aagaagaagc
     3781 actcaaacag tttaagcaaa aatttgatga ggtgctcagg gaaagctgga ctactaaagt
     3841 gaactggatg gcccacacag ttcggaaaga ctacagatct ttgccaactt tcttgtacaa
     3901 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     3961 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     4021 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     4081 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     4141 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     4201 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     4261 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     4321 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     4381 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     4441 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     4501 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     4561 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     4621 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     4681 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     4741 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     4801 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     4861 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     4921 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     4981 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     5041 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     5101 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     5161 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     5221 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc
     5281 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     5341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5521 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6001 gtt
//
