LOCUS       sequence-168751-        6558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB2_p.V842I
FEATURES             Location/Qualifiers
     source          1..6558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(918..945)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1037..1123)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1172..1194
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1186..1203
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1187..1203
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1219..1318
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5087..5186)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5203..5222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5204..5222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5227..5243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5227..5243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5674..6465
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(6558,1..588)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      841 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
      901 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
      961 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     1021 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     1081 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     1141 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     1201 agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     1261 caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa agttggcacc
     1321 atggagctgg cggccttgtg ccgctggggg ctcctcctcg ccctcttgcc ccccggagcc
     1381 gcgagcaccc aagtgtgcac cggcacagac atgaagctgc ggctccctgc cagtcccgag
     1441 acccacctgg acatgctccg ccacctctac cagggctgcc aggtggtgca gggaaacctg
     1501 gaactcacct acctgcccac caatgccagc ctgtccttcc tgcaggatat ccaggaggtg
     1561 cagggctacg tgctcatcgc tcacaaccaa gtgaggcagg tcccactgca gaggctgcgg
     1621 attgtgcgag gcacccagct ctttgaggac aactatgccc tggccgtgct agacaatgga
     1681 gacccgctga acaataccac ccctgtcaca ggggcctccc caggaggcct gcgggagctg
     1741 cagcttcgaa gcctcacaga gatcttgaaa ggaggggtct tgatccagcg gaacccccag
     1801 ctctgctacc aggacacgat tttgtggaag gacatcttcc acaagaacaa ccagctggct
     1861 ctcacactga tagacaccaa ccgctctcgg gcctgccacc cctgttctcc gatgtgtaag
     1921 ggctcccgct gctggggaga gagttctgag gattgtcaga gcctgacgcg cactgtctgt
     1981 gccggtggct gtgcccgctg caaggggcca ctgcccactg actgctgcca tgagcagtgt
     2041 gctgccggct gcacgggccc caagcactct gactgcctgg cctgcctcca cttcaaccac
     2101 agtggcatct gtgagctgca ctgcccagcc ctggtcacct acaacacaga cacgtttgag
     2161 tccatgccca atcccgaggg ccggtataca ttcggcgcca gctgtgtgac tgcctgtccc
     2221 tacaactacc tttctacgga cgtgggatcc tgcaccctcg tctgccccct gcacaaccaa
     2281 gaggtgacag cagaggatgg aacacagcgg tgtgagaagt gcagcaagcc ctgtgcccga
     2341 gtgtgctatg gtctgggcat ggagcacttg cgagaggtga gggcagttac cagtgccaat
     2401 atccaggagt ttgctggctg caagaagatc tttgggagcc tggcatttct gccggagagc
     2461 tttgatgggg acccagcctc caacactgcc ccgctccagc cagagcagct ccaagtgttt
     2521 gagactctgg aagagatcac aggttaccta tacatctcag catggccgga cagcctgcct
     2581 gacctcagcg tcttccagaa cctgcaagta atccggggac gaattctgca caatggcgcc
     2641 tactcgctga ccctgcaagg gctgggcatc agctggctgg ggctgcgctc actgagggaa
     2701 ctgggcagtg gactggccct catccaccat aacacccacc tctgcttcgt gcacacggtg
     2761 ccctgggacc agctctttcg gaacccgcac caagctctgc tccacactgc caaccggcca
     2821 gaggacgagt gtgtgggcga gggcctggcc tgccaccagc tgtgcgcccg agggcactgc
     2881 tggggtccag ggcccaccca gtgtgtcaac tgcagccagt tccttcgggg ccaggagtgc
     2941 gtggaggaat gccgagtact gcaggggctc cccagggagt atgtgaatgc caggcactgt
     3001 ttgccgtgcc accctgagtg tcagccccag aatggctcag tgacctgttt tggaccggag
     3061 gctgaccagt gtgtggcctg tgcccactat aaggaccctc ccttctgcgt ggcccgctgc
     3121 cccagcggtg tgaaacctga cctctcctac atgcccatct ggaagtttcc agatgaggag
     3181 ggcgcatgcc agccttgccc catcaactgc acccactcct gtgtggacct ggatgacaag
     3241 ggctgccccg ccgagcagag agccagccct ctgacgtcca tcatctctgc ggtggttggc
     3301 attctgctgg tcgtggtctt gggggtggtc tttgggatcc tcatcaagcg acggcagcag
     3361 aagatccgga agtacacgat gcggagactg ctgcaggaaa cggagctggt ggagccgctg
     3421 acacctagcg gagcgatgcc caaccaggcg cagatgcgga tcctgaaaga gacggagctg
     3481 aggaaggtga aggtgcttgg atctggcgct tttggcacag tctacaaggg catctggatc
     3541 cctgatgggg agaatgtgaa aattccagtg gccatcaaag tgttgaggga aaacacatcc
     3601 cccaaagcca acaaagaaat cttagacgaa gcatacgtga tggctggtgt gggctcccca
     3661 tatgtctccc gccttctggg catctgcctg acatccacgg tgcagctggt gacacagctt
     3721 atgccctatg gctgcctctt agaccatgtc cgggaaaacc gcggacgcct gggctcccag
     3781 gacctgctga actggtgtat gcagattgcc aaggggatga gctacctgga ggatgtgcgg
     3841 ctcatacaca gggacttggc cgctcggaac gtgctggtca agagtcccaa ccatgtcaaa
     3901 attacagact tcgggctggc tcggctgctg gacattgacg agacagagta ccatgcagat
     3961 gggggcaagg tgcccatcaa gtggatggcg ctggagtcca ttctccgccg gcggttcacc
     4021 caccagagtg atgtgtggag ttatggtgtg actgtgtggg agctgatgac ttttggggcc
     4081 aaaccttacg atgggatccc agcccgggag atccctgacc tgctggaaaa gggggagcgg
     4141 ctgccccagc cccccatctg caccattgat gtctacatga tcatggtcaa atgttggatg
     4201 attgactctg aatgtcggcc aagattccgg gagttggtgt ctgaattctc ccgcatggcc
     4261 agggaccccc agcgctttgt ggtcatccag aatgaggact tgggcccagc cagtcccttg
     4321 gacagcacct tctaccgctc actgctggag gacgatgaca tgggggacct ggtggatgct
     4381 gaggagtatc tggtacccca gcagggcttc ttctgtccag accctgcccc gggcgctggg
     4441 ggcatggtcc accacaggca ccgcagctca tctaccagga gtggcggtgg ggacctgaca
     4501 ctagggctgg agccctctga agaggaggcc cccaggtctc cactggcacc ctccgaaggg
     4561 gctggctccg atgtatttga tggtgacctg ggaatggggg cagccaaggg gctgcaaagc
     4621 ctccccacac atgaccccag ccctctacag cggtacagtg aggaccccac agtacccctg
     4681 ccctctgaga ctgatggcta cgttgccccc ctgacctgca gcccccagcc tgaatatgtg
     4741 aaccagccag atgttcggcc ccagccccct tcgccccgag agggccctct gcctgctgcc
     4801 cgacctgctg gtgccactct ggaaaggccc aagactctct ccccagggaa gaatggggtc
     4861 gtcaaagacg tttttgcctt tgggggtgcc gtggagaacc ccgagtactt gacaccccag
     4921 ggaggagctg cccctcagcc ccaccctcct cctgccttca gcccagcctt cgacaacctc
     4981 tattactggg accaggaccc accagagcgg ggggctccac ccagcacctt caaagggaca
     5041 cctacggcag agaacccaga gtacctgggt ctggacgtgc cagtgtgacc aactttcttg
     5101 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     5161 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     5221 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     5281 attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc
     5341 tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     5401 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     5461 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     5521 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     5581 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     5641 cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta
     5701 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     5761 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     5821 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     5881 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     5941 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     6001 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     6061 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     6121 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     6181 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     6241 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     6301 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     6361 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     6421 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca
     6481 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     6541 aagatcaaag gatcttct
//
