LOCUS       sequence-168755-        4605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MTM1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MTM1_p.R253Q
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     752..769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(928..955)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1047..1133)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1182..1204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1196..1213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1197..1213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1229..1328
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3144..3243)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3260..3279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3261..3279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3284..3300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3284..3300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3731..4522
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      781 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      841 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      901 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      961 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     1021 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     1081 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
     1141 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
     1201 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
     1261 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
     1321 agttggcacc atggcttctg catcaacttc taaatataat tcacactcct tggagaatga
     1381 gtctattaag aggacgtctc gagatggagt caatcgagat ctcactgagg ctgttcctcg
     1441 acttccagga gaaacactaa tcactgacaa agaagttatt tacatatgtc ctttcaatgg
     1501 ccccattaag ggaagagttt acatcacaaa ttatcgtctt tatttaagaa gtttggaaac
     1561 ggattcttct ctaatacttg atgttcctct gggtgtgatc tcgagaattg aaaaaatggg
     1621 aggcgcgaca agtagaggag aaaattccta tggtctagat attacttgta aagacatgag
     1681 aaacctgagg ttcgctttga aacaggaagg ccacagcaga agagatatgt ttgagatcct
     1741 cacgagatac gcgtttcccc tggctcacag tctgccatta tttgcatttt taaatgaaga
     1801 aaagtttaac gtggatggat ggacagttta caatccagtg gaagaataca ggaggcaggg
     1861 cttgcccaat caccattgga gaataacttt tattaataag tgctatgagc tctgtgacac
     1921 ttaccctgct cttttggtgg ttccgtatcg tgcctcagat gatgacctcc ggagagttgc
     1981 aacttttagg tcccgaaatc gaattccagt gctgtcatgg attcatccag aaaataagac
     2041 ggtcattgtg cgttgcagtc agcctcttgt cggtatgagt gggaaacaaa ataaagatga
     2101 tgagaaatat ctcgatgtta tcagggagac taataaacaa atttctaaac tcaccattta
     2161 tgatgcaaga cccagcgtaa atgcagtggc caacaaggca acaggaggag gatatgaaag
     2221 tgatgatgca tatcataacg ccgaactttt cttcttagac attcataata ttcatgttat
     2281 gcgggaatct ttaaaaaaag tgaaggacat tgtttatcct aatgtagaag aatctcattg
     2341 gttgtccagt ttggagtcta ctcattggtt agaacatatc aagctcgttt tgacaggagc
     2401 cattcaagta gcagacaaag tttcttcagg gaagagttca gtgcttgtgc attgcagtga
     2461 cggatgggac aggactgctc agctgacatc cttggccatg ctgatgttgg atagcttcta
     2521 taggagcatt gaagggttcg aaatactggt acaaaaaaaa tggataagtt ttggacataa
     2581 atttgcatct cgaataggtc atggtgataa aaaccacacc gatgctgacc gttctcctat
     2641 ttttctccag tttattgatt gtgtgtggca aatgtcaaaa cagttcccta cagcttttga
     2701 attcaatgaa caatttttga ttataatttt ggatcatctg tatagttgcc gatttggtac
     2761 tttcttattc aactgtgaat ctgctcgaga aagacagaag gttacagaaa ggactgtttc
     2821 tttatggtca ctgataaaca gtaataaaga aaaattcaaa aaccccttct atactaaaga
     2881 aatcaatcga gttttatatc cagttgccag tatgcgtcac ttggaactct gggtgaatta
     2941 ctacattaga tggaacccca ggatcaagca acaacagccg aatccagtgg agcagcgtta
     3001 catggagctc ttagccttac gcgacgaata cataaagcgg cttgaggaac tgcagctcgc
     3061 caactctgcc aagctttctg atcccccaac ttcaccttcc agtccttcgc aaatgatgcc
     3121 ccatgtgcaa actcacttct agttgccaac tttcttgtac aaagttggca ttataagaaa
     3181 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     3241 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     3301 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3361 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     3421 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3481 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3541 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3601 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3661 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3721 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3781 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3841 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3901 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3961 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     4021 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4081 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     4141 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     4201 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     4261 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     4321 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4381 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4441 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4501 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4561 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaa
//
