LOCUS       sequence-168771-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector for bi-cistronic expression of EGFP
            and the gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Lou E, Fujisawa S, Morozov A, Barlas A, Romin Y, Dogan Y, Gholami S,
            Moreira AL, Manova-Todorova K, Moore MA
  TITLE     Tunneling nanotubes provide a unique conduit for intercellular 
            transfer of cellular contents in human malignant pleural 
            mesothelioma.
  JOURNAL   PLoS One. 2012;7(3):e33093. doi: 10.1371/journal.pone.0033093. Epub 
            2012 Mar 9.
  PUBMED    22427958
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e33093. doi"; pages: " 
            10.1371/journal.pone.0033093. Epub 2012 Mar 9"
COMMENT     Sequence Label: pUltra
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..90
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             112..165
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    209..797
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(262..282)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(680..691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctcggcatgg acgagctgta caagggatct ggagcaacaa acttctcact actcaaacaa
       61 gcaggtgacg tggaggagaa tcccgggcct tctagaggat ccggctccgg cgagggcagg
      121 ggaagtctac taacatgcgg ggacgtggag gaaaatcccg gcccagctag cgtgtgatca
      181 gaattcgttc cggagtcgtc gactcgacaa tcaacctctg gattacaaaa tttgtgaaag
      241 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
      301 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
      361 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
      421 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
      481 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
      541 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
      601 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
      661 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
      721 gccggctctg cggcctcttc cgcatcttcg ccttcgccct cagacgagtc ggatctccct
      781 ttgggccgcc tccccgcctg gaattaattc gagctcggta cctttaagac caatgactta
      841 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctacg
      901 taactc
//
