LOCUS       sequence-168772-        5337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STAT6 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STAT6_p.D46N
FEATURES             Location/Qualifiers
     source          1..5337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3216..3315)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3332..3351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3333..3351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3356..3372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3356..3372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3803..4594
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4687..5275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5176..5195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgtctctgt ggggtctggt ctccaagatg cccccagaaa aagtgcagcg
      721 gctctatgtc gactttcccc aacacctgcg gcatcttctg ggtgactggc tggagagcca
      781 gccctgggag ttcctggtcg gctccaacgc cttctgctgc aacttggcta gtgccctact
      841 ttcagacact gtccagcacc ttcaggcctc ggtgggagag cagggggagg ggagcaccat
      901 cttgcaacac atcagcaccc ttgagagcat atatcagagg gaccccctga agctggtggc
      961 cactttcaga caaatacttc aaggagagaa aaaagctgtt atggaacagt tccgccactt
     1021 gccaatgcct ttccactgga agcaggaaga actcaagttt aagacaggct tgcggaggct
     1081 gcagcaccga gtaggggaga tccaccttct ccgagaagcc ctgcagaagg gggctgaggc
     1141 tggccaagtg tctctgcaca gcttgataga aactcctgct aatgggactg ggccaagtga
     1201 ggccctggcc atgctactgc aggagaccac tggagagcta gaggcagcca aagccctagt
     1261 gctgaagagg atccagattt ggaaacggca gcagcagctg gcagggaatg gcgcaccgtt
     1321 tgaggagagc ctggccccac tccaggagag gtgtgaaagc ctggtggaca tttattccca
     1381 gctacagcag gaggtagggg cggctggtgg ggagcttgag cccaagaccc gggcatcgct
     1441 gactggccgg ctggatgaag tcctgagaac cctcgtcacc agttgcttcc tggtggagaa
     1501 gcagcccccc caggtactga agactcagac caagttccag gctggagttc gattcctgtt
     1561 gggcttgagg ttcctggggg ccccagccaa gcctccgctg gtcagggccg acatggtgac
     1621 agagaagcag gcgcgggagc tgagtgtgcc tcagggtcct ggggctggag cagaaagcac
     1681 tggagaaatc atcaacaaca ctgtgccctt ggagaacagc attcctggga actgctgctc
     1741 tgccctgttc aagaacctgc ttctcaagaa gatcaagcgg tgtgagcgga agggcactga
     1801 gtctgtcaca gaggagaagt gcgctgtgct cttctctgcc agcttcacac ttggccccgg
     1861 caaactcccc atccagctcc aggccctgtc tctgcccctg gtggtcatcg tccatggcaa
     1921 ccaagacaac aatgccaaag ccactatcct gtgggacaat gccttctctg agatggaccg
     1981 cgtgcccttt gtggtggctg agcgggtgcc ctgggagaag atgtgtgaaa ctctgaacct
     2041 gaagttcatg gctgaggtgg ggaccaaccg ggggctgctc ccagagcact tcctcttcct
     2101 ggcccagaag atcttcaatg acaacagcct cagtatggag gccttccagc accgttctgt
     2161 gtcctggtcg cagttcaaca aggagatcct gctgggccgt ggcttcacct tttggcagtg
     2221 gtttgatggt gtcctggacc tcaccaaacg ctgtctccgg agctactggt ctgaccggct
     2281 gatcattggc ttcatcagca aacagtacgt tactagcctt cttctcaatg agcccgacgg
     2341 aacctttctc ctccgcttca gcgactcaga gattgggggc atcaccattg cccatgtcat
     2401 ccggggccag gatggctctc cacagataga gaacatccag ccattctctg ccaaagacct
     2461 gtccattcgc tcactggggg accgaatccg ggatcttgct cagctcaaaa atctctatcc
     2521 caagaagccc aaggatgagg ctttccggag ccactacaag cctgaacaga tgggtaagga
     2581 tggcaggggt tatgtcccag ctaccatcaa gatgaccgtg gaaagggacc aaccacttcc
     2641 taccccagag ctccagatgc ctaccatggt gccttcttat gaccttggaa tggcccctga
     2701 ttcctccatg agcatgcagc ttggcccaga tatggtgccc caggtgtacc caccacactc
     2761 tcactccatc cccccgtatc aaggcctctc cccagaagaa tcagtcaacg tgttgtcagc
     2821 cttccaggag cctcacctgc agatgccccc cagcctgggc cagatgagcc tgccctttga
     2881 ccagcctcac ccccagggcc tgctgccgtg ccagcctcag gagcatgctg tgtccagccc
     2941 tgaccccctg ctctgctcag atgtgaccat ggtggaagac agctgcctga gccagccagt
     3001 gacagcgttt cctcagggca cttggattgg tgaagacata ttccctcctc tgctgcctcc
     3061 cactgaacag gacctcacta agcttctcct ggaggggcaa ggggagtcgg ggggagggtc
     3121 cttgggggca cagcccctcc tgcagccctc ccactatggg caatctggga tctcaatgtc
     3181 ccacatggac ctaagggcca accccagttg gtgattgcca actttcttgt acaaagttgg
     3241 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     3301 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     3361 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3421 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     3481 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3541 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3601 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3661 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3721 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3781 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3841 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3901 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3961 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     4021 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     4081 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     4141 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     4201 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     4261 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     4321 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     4381 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     4441 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     4501 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4561 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4621 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4801 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5041 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
