LOCUS       sequence-168773-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 donor vector for genomic targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   DeKelver RC, Choi VM, Moehle EA, Paschon DE, Hockemeyer D, Meijsing 
            SH, Sancak Y, Cui X, Steine EJ, Miller JC, Tam P, Bartsevich VV, 
            Meng X, Rupniewski I, Gopalan SM, Sun HC, Pitz KJ, Rock JM, Zhang L,
            Davis GD, Rebar EJ, Cheeseman IM, Yamamoto KR, Sabatini DM, Jaenisch
            R, Gregory PD, Urnov FD
  TITLE     Functional genomics, proteomics, and regulatory DNA analysis in 
            isogenic settings using zinc finger nuclease-driven transgenesis 
            into a safe harbor locus in the human genome.
  JOURNAL   Genome Res. 2010 Aug;20(8):1133-42. doi: 10.1101/gr.106773.110. Epub
            2010 May 27.
  PUBMED    20508142
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.106773.110"; journalName: "Genome Res"; date: "2010-08";
            volume: "20"; issue: "8"; pages: "1133-42"
COMMENT     Sequence Label: AAVS1-Neo-M2rtTA
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(46..663,664..669,670..705,706..708,709..744,745..747,
                     748..783,784..792)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(826..845)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     880..899
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggcttgatcg aagctagtgg atcccccggg ctgcaggaat tcaccatgtc tagactggac
       61 aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg
      121 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
      181 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
      241 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
      301 ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
      361 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
      421 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
      481 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
      541 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
      601 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
      661 ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
      721 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
      781 ctccccgggt aactaagtaa ggatccagac atgataagat acattgatga gtttggacaa
      841 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      901 ttatttgtaa ccattataag
//
