LOCUS       sequence-168779-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin G76C for cross linking studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: Ubiquitin G76C
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..293)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     CDS             complement(297..317)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(333..356)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     gap             762..772
                     /estimated_length=11
     gap             777..792
                     /estimated_length=16
     gap             813..825
                     /estimated_length=13
     gap             846..856
                     /estimated_length=11
     gap             894..909
                     /estimated_length=16
     gap             926..937
                     /estimated_length=12
ORIGIN
        1 gnctttcgac tgagcctttc gttttatttg aagcttattt aattacctgc agggaattcg
       61 gatccttagc aacctcttag tcttaagaca agatgtaagg tcgactcctt ctgaatgttg
      121 taatcagaca gcgttctacc gtcctcgagc tgcttaccgg caaagatcaa tctttgttga
      181 tcaggtggaa ttccttcctt gtcttgaatt ttagccttaa cgttttcgat ggtatcggat
      241 ggttcaactt ccagagttat ggttttaccg gttaacgtct tgacgaagat ctgagcggac
      301 tgaaaataca ggttttcaga gctcgaatta gtctgcgcgt ctttcagggc ttcatcgaca
      361 gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac
      421 atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatagt ggcggcaata
      481 cgcggatctt tcaccaactc ttcctcgtaa gacttcagcg ctacggnncc cagcggtttg
      541 tctttattaa ccgcttccag accttcatca gtcagcanat agttttcgan nnncntttgc
      601 cagctnnttg ttcnnactnn nnnntnaata cntgnnntnn nnnnnnnacc naacggnngn
      661 nnnnaccntg angnnngnnn nnnacgntnc nncnnnatnn nttgnngnnn cnanntgnnc
      721 nngccnnnnn cntngannnn ancncnnntn ncntnntnan gnnnnnnnnn nnanngnnnn
      781 nnnnnnnnnn nntnnnnggn nnnnntnnac ngnnnnnnnn nnnnnaccnt tnnnnnnnnt
      841 nnnccnnnnn nnnnnnggna annnnnntnn nnnnnnanan nnncnnnnnn aaannnnnnn
      901 nnnnnnnnnt nnnnnnaann nnnntnnnnn nnnnnnncnn g
//
