LOCUS       sequence-16878-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a 3rd generation lentiviral transfer vector (highest titer) 
            from Nolan lab's FELIX vector system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     Nolan Lab Plasmids
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTiger
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     249..265
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     250..266
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             375..386
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     784..804
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggcccggatt ccgagacctc acaggtaaaa aagattacac agatactcga cagggttcaa
       61 tctcaaatat ttattgtatc tgtgggagcc tcaagggaga actcaaaagt cctcaacaaa
      121 gagactcctc gaagtttcac aaagcactgg ttacctagga gctgatcaat caggccttca
      181 gggttccaat actcatccca gtccaccctt ttttcttttt taggaggttc atcttcaggg
      241 gccccccctc gaggtcgacg gtatcgatgc ggggaggcgg cccaaaggga gatccgactc
      301 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
      361 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
      421 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
      481 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
      541 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
      601 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
      661 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
      721 gagaanatga aagccatacg ggaagcaata gcatgataca naggcattaa agcagcgtat
      781 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
      841 taatcc
//
