LOCUS       sequence-168780-        1208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin K6C/G76C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: Ubiquitin K6C/G76C
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..11
                     /estimated_length=10
     CDS             complement(81..302)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVCTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     CDS             complement(306..326)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(342..365)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     gap             1130..1144
                     /estimated_length=15
     gap             1190..1199
                     /estimated_length=10
ORIGIN
        1 gnnnnnnnnn nctttcgact gancctttcg ttttatttga agcttattta attacctgca
       61 gggaattcgg atccttagca acctcttagt cttaagacaa gatgtaaggt cgactccttc
      121 tgaatgttgt aatcagacag cgttctaccg tcctcgagct gcttaccggc aaagatcaat
      181 ctttgttgat caggtggaat tccttccttg tcttgaattt tagccttaac gttttcgatg
      241 gtatcggatg gttcaacttc cagagttatg gttttaccgg ttaacgtgca gacgaagatc
      301 tgagcggact gaaaatacag gttttcagag ctcgaattag tctgcgcgtc tttcagggct
      361 tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac ggcataccag
      421 aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatagtg
      481 gcggcaatac gcggatcttt caccaactct tcctcgtaag acttcagcgc tacggcaccc
      541 agcggtttgt ctttattaac cgcttccaga ccttcatcag tcagcagata gttttcgagg
      601 aactcttttg ccagctcttt gttcggactg gcggcgttaa tacctgcgct cagcacgcca
      661 acgaacggtt tggatggttg acccttgaag gtcggcagta ccgttacacc ataattcact
      721 ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc tgtttcgcct
      781 ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tttgttttta
      841 atcnntcaac caggaaggtc agacccgctt tcgcgccagc gttatccacg cccacgtctt
      901 taatgtcgta cttgccgttt tcatacttga acgcataacc cccgtcagca gcaatcagcg
      961 gccaggtgaa gtacgnttct tgcaggttga acatcagcgc gctcttacct ttcgctttca
     1021 gtnntttatn cngcncnggg ntctcntccc aggtttttgg nngggnncgg cancanatnt
     1081 tngnnnnnan ncancgatna cgcttnnann agcnatngnn nancnnncan nnnnnnnnnn
     1141 nnnnacggnn nncnnnnann gnanannnnn nngnncnnga anncgcnttn nnnnnnnnng
     1201 ggggggng
//
