LOCUS       sequence-168782-        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin K63C/G76C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: Ubiquitin K63C/G76C
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..290)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQCESTLHLVLRLRG"
     CDS             complement(294..314)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(330..353)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     gap             1118..1133
                     /estimated_length=16
     gap             1154..1167
                     /estimated_length=14
ORIGIN
        1 tttcgactga ncctttcgtt ttatttgaag cttatttaat tacctgcagg gaattcggat
       61 ccttagcaac ctcttagtct taagacaaga tgtaaggtcg actcgcactg aatgttgtaa
      121 tcagacagcg ttctaccgtc ctcgagctgc ttaccggcaa agatcaatct ttgttgatca
      181 ggtggaattc cttccttgtc ttgaatttta gccttaacgt tttcgatggt atcggatggt
      241 tcaacttcca gagttatggt tttaccggtt aacgtcttga cgaagatctg agcggactga
      301 aaatacaggt tttcagagct cgaattagtc tgcgcgtctt tcagggcttc atcgacagtc
      361 tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa agcggacatc
      421 tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt ccatagtggc ggcaatacgc
      481 ggatctttca ccaactcttc ctcgtaagac ttcagcgcta cggcacccag cggtttgtct
      541 ttattaaccg cttccagacc ttcatcagtc agcagatagt tttcgaggaa ctcttttgcc
      601 agctctttgt tcggactggc ggcgttaata cctgcgctca gcacgccaac gaacggtttg
      661 gatggttgac ccttgaaggt cggcagtacc gttacaccat aattcacttt gctggtgtcg
      721 atgttggacc atgcccacgg gccgttgatg gtcatcgctg tttcgccttt attaaaggca
      781 gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat cangtcaacc
      841 aggaanggtc agacccgctt tcgcgccagc gttatccacg cccacgtctt taatgtcgta
      901 cttgccgttt tcatacttga acgcataacc cccgtcagca gcaatcagcg ggccaggtga
      961 agtacggtnc ttgcagttga acatcagcgc gctcttacct ttcgctttca ntncnttnnt
     1021 ccagcncngg gnnctcntcc cnnnttttng ngnnnnnnng canatcttnn ntannaaanc
     1081 agcgannnnc ntncancann nntcgggnaa nnnannannn nnnnnnnnnn nnncnnnann
     1141 nnnnnncngn naannnnnnn nnnnnnntnn nntccnnngn nnnnncnntt nnt
//
