LOCUS       sequence-168783-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin K6C for cross linking studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: Ubiquitin K6C
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..295)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVCTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(299..319)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(335..358)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 cngtctttcg actgancctt tcgttttatt tgaagcttat ttaattacct gcagggaatt
       61 cggatcctta accacctctt agtcttaaga caagatgtaa ggtcgactcc ttctgaatgt
      121 tgtaatcaga cagcgttcta ccgtcctcga gctgcttacc ggcaaagatc aatctttgtt
      181 gatcaggtgg aattccttcc ttgtcttgaa ttttagcctt aacgttttcg atggtatcgg
      241 atggttcaac ttccagagtt atggttttac cggttaacgt gcagacgaag atctgagcgg
      301 actgaaaata caggttttca gagctcgaat tagtctgcgc gtctttcagg gcttcatcga
      361 cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac cagaaagcgg
      421 acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccata gtggcggcaa
      481 tacgcggatc tttcaccaac tcttcctcgt aagacttcag cgctacggca cccagcggtt
      541 tgtctttatt aaccgcttcc agaccttcat cagtcagcag atagttttcg aggaactctt
      601 ttgccagctc tttgttcgga ctggcggcgt taatacctgc gctcagcacg ccaacgaacg
      661 gnttggntgg ttgacccttg aaggtcggca gtaccgttac accataattc actttgctgg
      721 ngtcnatgtt ggaccatgcc cacgggccgt tganggtcat cgctgtttcg cctttntnaa
      781 ggcagcttct gcnatggagt atcgngtctg cattcatgng tttgttttan cngtcaacnn
      841 nanncananc cgcttcnnnn cagcntatcc ncgcccacgt cttatgncnn actgcntttc
      901 ntacttgang cataccccgt cagcagcatc annngnncng nna
//
