LOCUS       sequence-168784-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin K48C for cross linking studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: Ubiquitin K48C
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..293)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GCQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(297..317)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(333..356)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gncnnncgac tgagcctttc gttttatttg aagcttattt aattacctgc agggaattcg
       61 gatccttaac cacctcttag tcttaagaca agatgtaagg tcgactcctt ctgaatgttg
      121 taatcagaca gcgttctacc gtcctcgagc tggcaaccgg caaagatcaa tctttgttga
      181 tcaggtggaa ttccttcctt gtcttgaatt ttagccttaa cgttttcgat ggtatcggat
      241 ggttcaactt ccagagttat ggttttaccg gttaacgtct tgacgaagat ctgagcggac
      301 tgaaaataca ggttttcaga gctcgaatta gtctgcgcgt ctttcagggc ttcatcgaca
      361 gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac
      421 atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatagt ggcggcaata
      481 cgcggatctt tcaccaactc ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg
      541 tctttattaa ccgcttccag accttcatca gtcagcagat agttttcgag gaactctttt
      601 gccagctctt tgttcggact ggcggcgtta atacctgcgc tcagcacgcc aacgaacggt
      661 ttggntggnt gacccttgaa ggtcggcagt accgttacac cataattcac tttgctggtg
      721 tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc ttnattaaag
      781 gcagcttctg cgangganta atcgnngtct gcattcatgn gtttgtttta ncngtcacca
      841 gnnnntcana nccnctttcn cgcngcntat ccacgcccnc gtcttnntgn cgtantngnc
      901 nntttcatnc tgaacgnntn acccccgtca gcagcanncn ncgnnng
//
