LOCUS       sequence-168785-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin K63C for cross linking studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: Ubiquitin K63C
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..293)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQCESTLHLVLRLRGG"
     CDS             complement(297..317)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(333..356)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     gap             979..988
                     /estimated_length=10
ORIGIN
        1 gtctttcgac tgagcctttc gttttatttg aagcttattt aattacctgc agggaattcg
       61 gatccttaac cacctcttag tcttaagaca agatgtaagg tcgactcgca ctgaatgttg
      121 taatcagaca gcgttctacc gtcctcgagc tgcttaccgg caaagatcaa tctttgttga
      181 tcaggtggaa ttccttcctt gtcttgaatt ttagccttaa cgttttcgat ggtatcggat
      241 ggttcaactt ccagagttat ggttttaccg gttaacgtct tgacgaagat ctgagcggac
      301 tgaaaataca ggttttcaga gctcgaatta gtctgcgcgt ctttcagggc ttcatcgaca
      361 gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac
      421 atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatagt ggcggcaata
      481 cgcggatctt tcaccaactc ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg
      541 tctttattaa ccgcttccag accttcatca gtcagcagat agttttcgag gaactctttt
      601 gccagctctt tgttcggact ggcggcgtta atacctgcgc tcagcacgcc aacgaacggt
      661 ttggatggtt gacccttgaa ggtcggcagt accgttacac cataattcac tttgctggtg
      721 tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc tttattaaaa
      781 ggcagcttct gcgatggagt aatcggngtc tgcattcatg ngtttgtttt taatcnnnca
      841 accnggaann cagacccgct ttcgccncng cgttatccac nncccacgtn ntangncnna
      901 ctgncnnttt cnnnannnan nncannaccc cgtcagcagn ancnncgnnn nggngantan
      961 gnnnngnngn nnacntcann nnnnnnnnan cntnnnntnn nnnt
//
