LOCUS       sequence-168787-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This can be used to make MBP-TEV-Cterm with SapI and PstI; and can 
            also make N-term-VMA with NdeI and SapI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Liu J, Chen Q, Rozovsky S
  TITLE     Utilizing Selenocysteine for Expressed Protein Ligation and 
            Bioconjugations.
  JOURNAL   J Am Chem Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991.
  PUBMED    28186733
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2017 Feb 10. doi: 10.1021/jacs.6b10991."
COMMENT     Sequence Label: MBP-TEV-VMA Model vector
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(175..198)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ttnnnagacc catccgccat taaaacattg gtacccttgg caaagcagga agagcaccac
       61 cgcacgtttc cacacgcgca cgggtcaggc cactcagacg gttccaatcc gggcggtaag
      121 cggtgcccat gctcttccgc actgaaaata caggttttca gagctcgaat tagtctgcgc
      181 gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg
      241 cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc
      301 gttttccata gtggcggcaa tacgcggatc tttcaccaac tcttcctcgt aagacttcag
      361 cgctacggca cccagcggtt tgtctttatt aaccgcttcc agaccttcat cagtcagcag
      421 atagttttcg aggaactctt ttgccagctc tttgttcgga ctggcggcgt taatacctgc
      481 gctcagcacg ccaacgaacg gtttggatgg ttgacccttg aaggtcggca gtaccgttac
      541 accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat
      601 cgctgtttcg cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat
      661 gtgtttgttt ttaatcaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc
      721 cacgcccacg tctttaatgt cgtacttgcc gttttcatac ttgaacgcat aacccccgtc
      781 agcagcaatc agcggccagg tgaagtacgg ntcttgcnng ttgaacatca gcgcgctctt
      841 acctttcgct ttcagttctt tatccagcgc ccgggatctc ttcccaggtt tttggcgggt
      901 tcggcagcag atctttgtta taaatcagcg ataacgcttc acagcgatcg ggtaagcnat
      961 cagcttgccg ttgtancgta cggcatcc
//
