LOCUS       sequence-16879-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a 3rd generation lentiviral transfer vector (slightly lower 
            titer, highest expression level) from Nolan lab's FELIX vector 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  TITLE     Nolan Lab Plasmids
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLionII
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     248..264
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     249..265
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             374..385
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     783..803
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcccggattc cgagacctca caggtaaaaa agattacaca gatactcgac agggttcaat
       61 ctcaaatatt tattgtatct gtgggagcct caagggagaa ctcaaaagtc ctcaacaaag
      121 agactcctcg aagtttcaca aagcactggt tacctaggag ctgatcaatc aggccttcag
      181 ggttccaata ctcatcccag tccacccttt tttctttttt aggaggttca tcttcagggg
      241 ccccccctcg aggtcgacgg tatcgatgcg gggaggcggc ccaaagggag atccgactcg
      301 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
      361 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
      421 aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg
      481 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
      541 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
      601 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
      661 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
      721 agaanatgaa agccatacgg gaagcaatag catgatacan aggcattaaa gcagcgtatc
      781 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
      841 aatcc
//
