LOCUS       sequence-168795-        2684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli MutS expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Liu J, Hanne J, Britton BM, Bennett J, Kim D, Lee JB, Fishel R
  TITLE     Cascading MutS and MutL sliding clamps control DNA diffusion to 
            activate mismatch repair.
  JOURNAL   Nature. 2016 Nov 16;539(7630):583-587. doi: 10.1038/nature20562.
  PUBMED    27851738
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-11-16- 16"; volume: "539"; issue: "7630"; 
            pages: "583-587"
COMMENT     Sequence Label: pRAF-MutS
FEATURES             Location/Qualifiers
     source          1..2684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             599..610
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2603..2620
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgtttaactt taagaaggag atatacataa tatgagtgca atagaaaatt tcgacgccca
       61 tacgcccatg atgcagcagt atctcaggct gaaagcccag catcccgaga tcctgctgtt
      121 ttaccggatg ggtgattttt atgaactgtt ttatgacgac gcaaaacgcg cgtcgcaact
      181 gctggatatt tcactgacca aacgcggtgc ttcggcggga gagccgatcc cgatggcggg
      241 gattccctac catgcggtgg aaaactatct cgccaaactg gtgaatcagg gagagtccgt
      301 tgccatctgc gaacaaattg gcgatccggc gaccagcaaa ggtccggttg agcgcaaagt
      361 tgtgcgtatc gttacgccag gcaccatcag cgatgaagcc ctgttgcagg agcgtcagga
      421 caacctgctg gcggctatct ggcaggacag caaaggtttc ggctacgcga cgctggatat
      481 cagttccggg cgttttcgcc tgagcgaacc ggctgaccgc gaaacgatgg cggcagaact
      541 gcaacgcact aatcctgcgg aactgctgta tgcagaagat tttgctgaaa tgtcgttaat
      601 tgaaggccgt cgcggcctgc gccgtcgccc gctgtgggag tttgaaatcg acaccgcgcg
      661 ccagcagttg aatctgcaat ttgggacccg cgatctggtc ggttttggcg tcgagaacgc
      721 gccgcgcgga ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgtac
      781 gactctgccg catattcgtt ccatcaccat ggaacgtgag caggacagca tcattatgga
      841 tgccgcgacg cgtcgtaatc tggaaatcac ccagaacctg gcgggtggtg cggaaaatac
      901 gctggcttct gtgctcgact gcaccgtcac gccgatgggc agccgtatgc tgaaacgctg
      961 gctgcatatg ccagtgcgcg atacccgcgt gttgcttgag cgccagcaaa ctattggcgc
     1021 attgcaggat ttcaccgccg ggctacagcc ggtactgcgt caggtcggcg acctggaacg
     1081 tattctggca cgtctggctt tacgaactgc tcgcccacgc gatctggccc gtatgcgcca
     1141 cgctttccag caactgccgg agctgcgtgc gcagttagaa actgtcgata gtgcaccggt
     1201 acaggcgcta cgtgagaaga tgggcgagtt tgccgagctg cgcgatctgc tggagcgagc
     1261 aatcatcgac acaccgccgg tgctggtacg cgacggtggt gttatcgcat cgggctataa
     1321 cgaagagctg gatgagtggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct
     1381 ggaagtccgc gagcgtgaac gtaccggcct ggacacgctg aaagttggct ttaatgcggt
     1441 gcacggctac tacattcaaa tcagccgtgg gcaaagccat ctggcaccca tcaactacat
     1501 gcgtcgccag acgctgaaaa acgccgagcg ctacatcatt ccagagctaa aagagtacga
     1561 agataaagtt ctcacctcaa aaggcaaagc actggcactg gaaaaacagc tttatgaaga
     1621 gctgttcgac ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc
     1681 ggaactcgac gtgctggtta acctggcgga acgggcctat accctgaact acacctgccc
     1741 gaccttcatt gataaaccgg gcattcgcat taccgaaggt cgccatccgg tagttgaaca
     1801 agtactgaat gagccattta tcgccaaccc gctgaatctg tcgccgcagc gccgcatgtt
     1861 gatcatcacc ggtccgaaca tgggcggtaa aagtacctat atgcgccaga ccgcactgat
     1921 tgcgctgatg gcctacatcg gcagctatgt accggcacaa aaagtcgaga ttggacctat
     1981 cgatcgcatc tttacccgcg taggcgcggc agatgacctg gcgtccgggc gctcaacctt
     2041 tatggtggag atgactgaaa ccgccaatat tttacataac gccaccgaat acagtctggt
     2101 gttaatggat gagatcgggc gtggaacgtc cacctacgat ggtctgtcgc tggcgtgggc
     2161 gtgcgcggaa aatctggcga ataagattaa ggcattgacg ttatttgcta cccactattt
     2221 cgagctgacc cagttaccgg agaaaatgga aggcgtcgct aacgtgcatc tcgatgcact
     2281 ggagcacggc gacaccattg cctttatgca cagcgtgcag gatggcgcgg cgagcaaaag
     2341 ctacggcctg gcggttgcag ctctggcagg cgtgccaaaa gaggttatta agcgcgcacg
     2401 gcaaaagctg cgtgagctgg aaagcatttc gccgaacgcc gccgctacgc aagtggatgg
     2461 tacgcaaatg tctttgctgt cagtaccaga agaaacttcg cctgcggtcg aagctctgga
     2521 aaatcttgat ccgcgttcac tcaccccgga acaggcgctg gagtggattt atcgcttgaa
     2581 gagcctggtg ggagggggtg gccatcacca tcaccatcat agggggctct gcacaccgtc
     2641 gcgttaagga tccgaattcg agctccgtcg acaagcttgc ggcc
//
