LOCUS       sequence-168808-        5139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.G34E
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     520..539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     773..790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(949..976)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1068..1154)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1203..1225
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1217..1234
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1218..1234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1250..1349
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1352..3697
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSEIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3699..3798)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3815..3834)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3816..3834)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3839..3855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3839..3855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4286..5077
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       61 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      661 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      721 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      781 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      841 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      901 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
      961 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     1021 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     1081 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     1141 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     1201 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
     1261 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
     1321 aatgtcaact ttgtacaaaa aagttggcac catggctact caagctgatt tgatggagtt
     1381 ggacatggcc atggaaccag acagaaaagc ggctgttagt cactggcagc aacagtctta
     1441 cctggactct gaaatccatt ctggtgccac taccacagct ccttctctga gtggtaaagg
     1501 caatcctgag gaagaggatg tggatacctc ccaagtcctg tatgagtggg aacagggatt
     1561 ttctcagtcc ttcactcaag aacaagtagc tgatattgat ggacagtatg caatgactcg
     1621 agctcagagg gtacgagctg ctatgttccc tgagacatta gatgagggca tgcagatccc
     1681 atctacacag tttgatgctg ctcatcccac taatgtccag cgtttggctg aaccatcaca
     1741 gatgctgaaa catgcagttg taaacttgat taactatcaa gatgatgcag aacttgccac
     1801 acgtgcaatc cctgaactga caaaactgct aaatgacgag gaccaggtgg tggttaataa
     1861 ggctgcagtt atggtccatc agctttctaa aaaggaagct tccagacacg ctatcatgcg
     1921 ttctcctcag atggtgtctg ctattgtacg taccatgcag aatacaaatg atgtagaaac
     1981 agctcgttgt accgctggga ccttgcataa cctttcccat catcgtgagg gcttactggc
     2041 catctttaag tctggaggca ttcctgccct ggtgaaaatg cttggttcac cagtggattc
     2101 tgtgttgttt tatgccatta caactctcca caacctttta ttacatcaag aaggagctaa
     2161 aatggcagtg cgtttagctg gtgggctgca gaaaatggtt gccttgctca acaaaacaaa
     2221 tgttaaattc ttggctatta cgacagactg ccttcaaatt ttagcttatg gcaaccaaga
     2281 aagcaagctc atcatactgg ctagtggtgg accccaagct ttagtaaata taatgaggac
     2341 ctatacttac gaaaaactac tgtggaccac aagcagagtg ctgaaggtgc tatctgtctg
     2401 ctctagtaat aagccggcta ttgtagaagc tggtggaatg caagctttag gacttcacct
     2461 gacagatcca agtcaacgtc ttgttcagaa ctgtctttgg actctcagga atctttcaga
     2521 tgctgcaact aaacaggaag ggatggaagg tctccttggg actcttgttc agcttctggg
     2581 ttcagatgat ataaatgtgg tcacctgtgc agctggaatt ctttctaacc tcacttgcaa
     2641 taattataag aacaagatga tggtctgcca agtgggtggt atagaggctc ttgtgcgtac
     2701 tgtccttcgg gctggtgaca gggaagacat cactgagcct gccatctgtg ctcttcgtca
     2761 tctgaccagc cgacaccaag aagcagagat ggcccagaat gcagttcgcc ttcactatgg
     2821 actaccagtt gtggttaagc tcttacaccc accatcccac tggcctctga taaaggctac
     2881 tgttggattg attcgaaatc ttgccctttg tcccgcaaat catgcacctt tgcgtgagca
     2941 gggtgccatt ccacgactag ttcagttgct tgttcgtgca catcaggata cccagcgccg
     3001 tacgtccatg ggtgggacac agcagcaatt tgtggagggg gtccgcatgg aagaaatagt
     3061 tgaaggttgt accggagccc ttcacatcct agctcgggat gttcacaacc gaattgttat
     3121 cagaggacta aataccattc cattgtttgt gcagctgctt tattctccca ttgaaaacat
     3181 ccaaagagta gctgcagggg tcctctgtga acttgctcag gacaaggaag ctgcagaagc
     3241 tattgaagct gagggagcca cagctcctct gacagagtta cttcactcta ggaatgaagg
     3301 tgtggcgaca tatgcagctg ctgttttgtt ccgaatgtct gaggacaagc cacaagatta
     3361 caagaaacgg ctttcagttg agctgaccag ctctctcttc agaacagagc caatggcttg
     3421 gaatgagact gctgatcttg gacttgatat tggtgcccag ggagaacccc ttggatatcg
     3481 ccaggatgat cctagctatc gttcttttca ctctggtgga tatggccagg atgccttggg
     3541 tatggacccc atgatggaac atgagatggg tggccaccac cctggtgctg actatccagt
     3601 tgatgggctg ccagatctgg ggcatgccca ggacctcatg gatgggctgc ctccaggtga
     3661 cagcaatcag ctggcctggt ttgatactga cctgtaattg ccaactttct tgtacaaagt
     3721 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     3781 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3841 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3901 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     3961 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     4021 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     4081 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     4141 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     4201 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     4261 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     4321 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     4381 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     4441 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     4501 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     4561 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     4621 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     4681 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     4741 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     4801 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     4861 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     4921 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     4981 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     5041 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     5101 atcccttaac gtgagttacg cgtcgttcca ctgagcgtc
//
