LOCUS       sequence-168812-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP2K1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP2K1_p.E203K
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      47..635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     536..555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     789..806
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(965..992)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1084..1170)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1219..1241
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1233..1250
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1234..1250
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1266..1365
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2551..2650)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2667..2686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2668..2686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2691..2707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2691..2707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3138..3929
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
       61 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      121 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      181 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      241 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      301 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      361 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      421 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      481 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      541 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      601 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      661 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      721 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      781 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      841 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      901 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
      961 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     1021 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     1081 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     1141 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     1201 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     1261 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
     1321 agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcaccatg cccaagaaga
     1381 agccgacgcc catccagctg aacccggccc ccgacggctc tgcagttaac gggaccagct
     1441 ctgcggagac caacttggag gccttgcaga agaagctgga ggagctagag cttgatgagc
     1501 agcagcgaaa gcgccttgag gcctttctta cccagaagca gaaggtggga gaactgaagg
     1561 atgacgactt tgagaagatc agtgagctgg gggctggcaa tggcggtgtg gtgttcaagg
     1621 tctcccacaa gccttctggc ctggtcatgg ccagaaagct aattcatctg gagatcaaac
     1681 ccgcaatccg gaaccagatc ataagggagc tgcaggttct gcatgagtgc aactctccgt
     1741 acatcgtggg cttctatggt gcgttctaca gcgatggcga gatcagtatc tgcatggagc
     1801 acatggatgg aggttctctg gatcaagtcc tgaagaaagc tggaagaatt cctgaacaaa
     1861 ttttaggaaa agttagcatt gctgtaataa aaggcctgac atatctgagg gagaagcaca
     1921 agatcatgca cagagatgtc aagccctcca acatcccagt caactcccgt gggaagatca
     1981 agctctgtga ctttggggtc agcgggcagc tcatcgactc catggccaac tccttcgtgg
     2041 gcacaaggtc ctacatgtcg ccagaaagac tccaggggac tcattactct gtgcagtcag
     2101 acatctggag catgggactg tctctggtag agatggcggt tgggaggtat cccatccctc
     2161 ctccagatgc caaggagctg gagctgatgt ttgggtgcca ggtggaagga gatgcggctg
     2221 agaccccacc caggccaagg acccccggga ggccccttag ctcatacgga atggacagcc
     2281 gacctcccat ggcaaatttt gagttgttgg attacatagt caacgagcct cctccaaaac
     2341 tgcccagtgg agtgttcagt ctggaatttc aagattttgt gaataaatgc ttaataaaaa
     2401 accccgcaga gagagcagat ttgaagcaac tcatggttca tgcttttatc aagagatctg
     2461 atgctgagga agtggatttt gcaggttggc tctgctccac catcggcctt aaccagccca
     2521 gcacaccaac ccatgctgct ggcgtctgat tgccaacttt cttgtacaaa gttggcatta
     2581 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2641 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     2701 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2761 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2821 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2881 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2941 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     3001 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     3061 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     3121 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     3181 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     3241 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     3301 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     3361 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     3421 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     3481 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3541 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     3601 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3661 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3721 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3781 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3841 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3901 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3961 acgtgagtta cgcgt
//
