LOCUS       sequence-168816-        4119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HTR3B, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HTR3B_p.I118T
FEATURES             Location/Qualifiers
     source          1..4119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(932..959)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1051..1137)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1186..1208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1200..1217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1201..1217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1233..1332
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2662..2761)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2778..2797)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2779..2797)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2802..2818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2802..2818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3249..4040
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
      901 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      961 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     1021 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     1081 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     1141 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
     1201 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
     1261 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
     1321 aaaaagttgg caccatgttg tcaagtgtaa tggctcccct gtgggcctgc atcctggtgg
     1381 ctgcaggaat tctagccaca gatacacatc atccccagga ttctgctctg tatcatctca
     1441 gcaagcagct attacagaaa tatcataaag aagtgagacc tgtttacaac tggaccaagg
     1501 ccaccacagt ctacctggac ctgttcgtcc atgctatatt ggatgtggat gcagagaatc
     1561 aaatattaaa gacaagtgta tggtaccaag aggtctggaa tgatgaattt ttatcctgga
     1621 actccagcat gtttgatgag attagagaga tctccctacc tctaagtgcc atctgggccc
     1681 ccgataccat catcaatgag tttgtggaca ttgaaagata ccctgacctt ccctatgttt
     1741 atgtgaactc atctgggacc attgagaact ataagcccat ccaggtggtc tctgcgtgca
     1801 gtttagagac atatgctttt ccatttgatg tccagaattg cagcctgacc ttcaagagca
     1861 ttctgcatac agtggaagac gtagacctgg cctttctgag gagcccagaa gacattcagc
     1921 atgacaaaaa ggcgtttttg aatgacagtg agtgggaact tctatctgtg tcctccacat
     1981 acagcatcct gcagagcagc gctggaggat ttgcacagat tcagtttaat gtggtgatgc
     2041 gcaggcaccc cctggtctat gtcgtgagtc tgctgattcc tagcatcttt ctcatgctgg
     2101 tggacctggg gagcttctac ctgccaccca actgccgagc caggattgtg ttcaagacca
     2161 gtgtgctggt gggctacacc gtcttcaggg tcaacatgtc caaccaggtg ccacggagtg
     2221 tagggagcac ccctctgatt gggcacttct tcaccatctg catggccttc ttggttctca
     2281 gcttagctaa gtccatcgtg ttggtcaaat tcctccatga tgagcagcgt ggtggacagg
     2341 agcagccctt cttgtgcctt cgaggggaca ccgatgctga caggcctaga gtggaaccca
     2401 gggcccaacg tgctgtggta acagagtcct cgctgtatgg agagcacctg gcccagccag
     2461 gaaccctgaa ggaagtctgg tcgcagcttc aatctatcag caactacctc caaactcagg
     2521 accagacaga ccaacaggag gcagagtggc tggtcctcct gtcccgcttt gaccgactgc
     2581 tcttccaaag ctaccttttc atgctgggga tctacaccat cactctgtgc tccctctggg
     2641 cactgtgggg cggcgtgtga ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     2701 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     2761 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     2821 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     2881 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     2941 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     3001 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     3061 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     3121 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     3181 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     3241 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     3301 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     3361 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     3421 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     3481 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     3541 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3601 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3661 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3721 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3781 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3841 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3901 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3961 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     4021 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     4081 acgcgtcgtt ccactgagcg tcagaccccg tagaaaaga
//
