LOCUS       sequence-168824-        3077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing EZH2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EZH2_WT
FEATURES             Location/Qualifiers
     source          1..3077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..117
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2371..2470)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2487..2506)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2488..2506)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2511..2527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2511..2527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatgg
      121 gccagactgg gaagaaatct gagaagggac cagtttgttg gcggaagcgt gtaaaatcag
      181 agtacatgcg actgagacag ctcaagaggt tcagacgagc tgatgaagta aagagtatgt
      241 ttagttccaa tcgtcagaaa attttggaaa gaacggaaat cttaaaccaa gaatggaaac
      301 agcgaaggat acagcctgtg cacatcctga cttctgtgag ctcattgcgc gggactaggg
      361 agtgttcggt gaccagtgac ttggattttc caacacaagt catcccatta aagactctga
      421 atgcagttgc ttcagtaccc ataatgtatt cttggtctcc cctacagcag aattttatgg
      481 tggaagatga aactgtttta cataacattc cttatatggg agatgaagtt ttagatcagg
      541 atggtacttt cattgaagaa ctaataaaaa attatgatgg gaaagtacac ggggatagag
      601 aatgtgggtt tataaatgat gaaatttttg tggagttggt gaatgccctt ggtcaatata
      661 atgatgatga cgatgatgat gatggagacg atcctgaaga aagagaagaa aagcagaaag
      721 atctggagga tcaccgagat gataaagaaa gccgcccacc tcggaaattt ccttctgata
      781 aaatttttga agccatttcc tcaatgtttc cagataaggg cacagcagaa gaactaaagg
      841 aaaaatataa agaactcacc gaacagcagc tcccaggcgc acttcctcct gaatgtaccc
      901 ccaacataga tggaccaaat gctaaatctg ttcagagaga gcaaagctta cactcctttc
      961 atacgctttt ctgtaggcga tgttttaaat atgactgctt cctacatcgt aagtgcaatt
     1021 attcttttca tgcaacaccc aacacttata agcggaagaa cacagaaaca gctctagaca
     1081 acaaaccttg tggaccacag tgttaccagc atttggaggg agcaaaggag tttgctgctg
     1141 ctctcaccgc tgagcggata aagaccccac caaaacgtcc aggaggccgc agaagaggac
     1201 ggcttcccaa taacagtagc aggcccagca cccccaccat taatgtgctg gaatcaaagg
     1261 atacagacag tgatagggaa gcagggactg aaacgggggg agagaacaat gataaagaag
     1321 aagaagagaa gaaagatgaa acttcgagct cctctgaagc aaattctcgg tgtcaaacac
     1381 caataaagat gaagccaaat attgaacctc ctgagaatgt ggagtggagt ggtgctgaag
     1441 cctcaatgtt tagagtcctc attggcactt actatgacaa tttctgtgcc attgctaggt
     1501 taattgggac caaaacatgt agacaggtgt atgagtttag agtcaaagaa tctagcatca
     1561 tagctccagc tcccgctgag gatgtggata ctcctccaag gaaaaagaag aggaaacacc
     1621 ggttgtgggc tgcacactgc agaaagatac agctgaaaaa ggacggctcc tctaaccatg
     1681 tttacaacta tcaaccctgt gatcatccac ggcagccttg tgacagttcg tgcccttgtg
     1741 tgatagcaca aaatttttgt gaaaagtttt gtcaatgtag ttcagagtgt caaaaccgct
     1801 ttccgggatg ccgctgcaaa gcacagtgca acaccaagca gtgcccgtgc tacctggctg
     1861 tccgagagtg tgaccctgac ctctgtctta cttgtggagc cgctgaccat tgggacagta
     1921 aaaatgtgtc ctgcaagaac tgcagtattc agcggggctc caaaaagcat ctattgctgg
     1981 caccatctga cgtggcaggc tgggggattt ttatcaaaga tcctgtgcag aaaaatgaat
     2041 tcatctcaga atactgtgga gagattattt ctcaagatga agctgacaga agagggaaag
     2101 tgtatgataa atacatgtgc agctttctgt tcaacttgaa caatgatttt gtggtggatg
     2161 caacccgcaa gggtaacaaa attcgttttg caaatcattc ggtaaatcca aactgctatg
     2221 caaaagttat gatggttaac ggtgatcaca ggataggtat ttttgccaag agagccatcc
     2281 agactggcga agagctgttt tttgattaca gatacagcca ggctgatgcc ctgaagtatg
     2341 tcggcatcga aagagaaatg gaaatccctt gcccaacttt cttgtacaaa gttggcatta
     2401 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2461 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     2521 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2581 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2641 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2701 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2761 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2821 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2881 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2941 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     3001 aggtagttgg cgtcatcgag cgcmatctcg aaccgacgtt gctggccgta catttgtacg
     3061 gctccgcagt ggatggc
//
