LOCUS       sequence-168827-        4089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing STK11 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_STK11_WT
FEATURES             Location/Qualifiers
     source          1..4089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1968..2067)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2084..2103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2085..2103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2108..2124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2108..2124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2555..3346
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3439..4027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3928..3947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gaggtggtgg acccgcagca gctgggcatg ttcacggagg gcgagctgat
      721 gtcggtgggt atggacacgt tcatccaccg catcgactcc accgaggtca tctaccagcc
      781 gcgccgcaag cgggccaagc tcatcggcaa gtacctgatg ggggacctgc tgggggaagg
      841 ctcttacggc aaggtgaagg aggtgctgga ctcggagacg ctgtgcagga gggccgtcaa
      901 gatcctcaag aagaagaagt tgcgaaggat ccccaacggg gaggccaacg tgaagaagga
      961 aattcaacta ctgaggaggt tacggcacaa aaatgtcatc cagctggtgg atgtgttata
     1021 caacgaagag aagcagaaaa tgtatatggt gatggagtac tgcgtgtgtg gcatgcagga
     1081 aatgctggac agcgtgccgg agaagcgttt cccagtgtgc caggcccacg ggtacttctg
     1141 tcagctgatt gacggcctgg agtacctgca tagccagggc attgtgcaca aggacatcaa
     1201 gccggggaac ctgctgctca ccaccggtgg caccctcaaa atctccgacc tgggcgtggc
     1261 cgaggcactg cacccgttcg cggcggacga cacctgccgg accagccagg gctccccggc
     1321 tttccagccg cccgagattg ccaacggcct ggacaccttc tccggcttca aggtggacat
     1381 ctggtcggct ggggtcaccc tctacaacat caccacgggt ctgtacccct tcgaagggga
     1441 caacatctac aagttgtttg agaacatcgg gaaggggagc tacgccatcc cgggcgactg
     1501 tggccccccg ctctctgacc tgctgaaagg gatgcttgag tacgaaccgg ccaagaggtt
     1561 ctccatccgg cagatccggc agcacagctg gttccggaag aaacatcctc cggctgaagc
     1621 accagtgccc atcccaccga gcccagacac caaggaccgg tggcgcagca tgactgtggt
     1681 gccgtacttg gaggacctgc acggcgcgga cgaggacgag gacctcttcg acatcgagga
     1741 tgacatcatc tacactcagg acttcacggt gcccggacag gtcccagaag aggaggccag
     1801 tcacaatgga cagcgccggg gcctccccaa ggccgtgtgt atgaacggca cagaggcggc
     1861 gcagctgagc accaaatcca gggcggaggg ccgggccccc aaccctgccc gcaaggcctg
     1921 ctccgccagc agcaagatcc gccggctgtc ggcctgcaag cagcagtgcc caactttctt
     1981 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2041 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2101 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2161 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     2221 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2281 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     2341 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     2401 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     2461 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2521 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     2581 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2641 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     2701 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2761 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2821 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2881 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2941 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3001 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3061 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3121 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3181 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3241 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3301 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     3361 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3481 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3541 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3601 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3661 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3781 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3961 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4021 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4081 tcacatgtt
//
