LOCUS       sequence-168828-        4155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TCF7L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TCF7L2_WT
FEATURES             Location/Qualifiers
     source          1..4155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2034..2133)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2150..2169)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2151..2169)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2174..2190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2174..2190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2621..3412
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3505..4093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3994..4013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg ccgcagctga acggcggtgg aggggatgac ctaggcgcca acgacgaact
      721 gatttccttc aaagacgagg gcgaacagga ggagaagagc tccgaaaact cctcggcaga
      781 gagggattta gctgatgtca aatcgtctct agtcaatgaa tcagaaacga atcaaaacag
      841 ctcctccgat tccgaggcgg aaagacggcc tccgcctcgc tccgaaagtt tccgagacaa
      901 atcccgggaa agtttggaag aagcggccaa gaggcaagat ggagggctct ttaaggggcc
      961 accgtatccc ggctacccct tcatcatgat ccccgacctg acgagcccct acctccccaa
     1021 cggatcgctc tcgcccaccg cccgaaccta tctccagatg aaatggccac tgcttgatgt
     1081 ccaggcaggg agcctccaga gtagacaagc cctcaaggat gcccggtccc catcaccggc
     1141 acacattgtc tctaacaaag tgccagtggt gcagcaccct caccatgtcc accccctcac
     1201 gcctcttatc acgtacagca atgaacactt cacgccggga aacccacctc cacacttacc
     1261 agccgacgta gaccccaaaa caggaatccc acggcctccg caccctccag atatatcccc
     1321 gtattaccca ctatcgcctg gcaccgtagg acaaatcccc catccgctag gatggcaagg
     1381 tcaaccagtg tacccaatca cgacaggagg attcagacac ccctacccca cagctctgac
     1441 cgtcaatgct tccatgtcca ggttccctcc ccatatggtc ccaccacatc atacgctaca
     1501 cacgacgggc attccgcatc cggccatagt cacaccaaca gtcaaacagg aatcgtccca
     1561 gagtgatgtc ggctcactcc atagttcaaa gcatcaggac tccaaaaagg aagaagaaaa
     1621 gaagaagccc cacataaaga aacctcttaa tgcattcatg ttgtatatga aggaaatgag
     1681 agcaaaggtc gtagctgagt gcacgttgaa agaaagcgcg gccatcaacc agatccttgg
     1741 gcggaggtgg catgcactgt ccagagaaga gcaagcgaaa tactacgagc tggcccggaa
     1801 ggagcgacag cttcatatgc aactgtaccc cggctggtcc gcgcgggata actatggaaa
     1861 gaagaagaag aggaaaaggg acaagcagcc gggagagacc aatgaacaca gcgaatgttt
     1921 cctaaatcct tgcctttcac ttcctccgat tacaggagaa aaaaaaagtg cgttcgctac
     1981 atacaaggtg aaggcagctg cctcagccca ccctcttcag atggaagctt actacccaac
     2041 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2101 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2161 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2221 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     2281 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
     2341 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     2401 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     2461 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     2521 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     2581 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc
     2641 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2701 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     2761 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     2821 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     2881 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     2941 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3001 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3061 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3121 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3181 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3241 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3301 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3361 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     3421 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     3481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     3601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     3661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     3721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4141 ttttgctcac atgtt
//
