LOCUS       sequence-168832-        4977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SV2C, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SV2C_WT
FEATURES             Location/Qualifiers
     source          1..4977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     520..539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     773..790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(949..976)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1068..1154)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1203..1225
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1217..1234
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1218..1234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1250..1349
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3537..3636)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3653..3672)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3654..3672)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3677..3693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3677..3693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4124..4915
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       61 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
      181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      661 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      721 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      781 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      841 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      901 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
      961 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     1021 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     1081 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     1141 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     1201 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
     1261 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
     1321 aatgccaact ttgtacaaaa aagttggcac catggaagac tcttacaagg ataggacttc
     1381 actgatgaag ggtgccaagg acattgccag agaggtgaag aaacaaacag taaagaaggt
     1441 gaatcaagct gtggaccgag cccaggatga atacacccag aggtcctaca gtcggttcca
     1501 agatgaagaa gatgatgatg actactaccc ggctggagaa acctataatg gtgaggccaa
     1561 cgatgacgaa ggctcaagtg aagccactga ggggcatgat gaagatgatg agatctatga
     1621 gggggagtat cagggcatcc ccagtatgaa ccaagcgaag gacagcatcg tgtcagtggg
     1681 gcagcccaag ggcgatgagt acaaggaccg acgggagctg gaatcagaaa ggagagctga
     1741 cgaggaagag ttagcccagc agtatgagct gataatccaa gaatgcggtc atggtcgttt
     1801 tcagtgggcc cttttcttcg tcctgggcat ggctcttatg gcagacggtg tagaggtgtt
     1861 tgtcgttggc ttcgtgttac ccagtgctga gacagacctc tgcatcccaa attcaggatc
     1921 tggatggcta ggcagcatag tgtacctcgg gatgatggtg ggggcgttct tctggggagg
     1981 actggcagac aaagtgggaa ggaaacagtc tcttctgatt tgcatgtctg tcaacggatt
     2041 ctttgccttc ctttcttcat ttgtccaagg ttatggcttc tttctcttct gtcgcttact
     2101 ttctggattc gggattggag gagccatacc cactgtgttc tcgtactttg ctgaagtcct
     2161 ggcccgggaa aagcggggcg aacacttgag ctggctctgc atgttctgga tgatcggtgg
     2221 catctacgcc tctgccatgg cctgggccat catcccgcac tacgggtgga gcttcagcat
     2281 gggatcggcc taccagtttc acagttggcg tgtgtttgtc atcgtctgtg cactcccctg
     2341 tgtctcctcc gtggtggccc tcacattcat gcctgaaagc ccacgatcct tgttggaggt
     2401 tggaaaacat gatgaagctt ggatgattct gaagttaatt catgacacca acatgagagc
     2461 ccggggtcag cctgagaagg tcttcacggt aaacaaaata aaaactccta aacaaataga
     2521 tgagctgatt gaaattgaga gtgacacagg aacatggtat aggaggtgtt ttgttcggat
     2581 ccgcaccgag ctgtacggaa tttggttgac ttttatgaga tgtttcaact acccagtcag
     2641 ggataataca ataaagctta caattgtttg gttcaccctg tcctttgggt actatggatt
     2701 atccgtttgg ttccctgatg tcattaaacc tctgcagtcc gatgaatatg cattgctaac
     2761 cagaaatgtg gagagagata aatatgcaaa tttcactatt aactttacaa tggaaaatca
     2821 gattcatact ggaatggaat acgacaatgg cagattcata ggggtcaagt tcaaatctgt
     2881 aactttcaaa gactctgttt ttaagtcctg cacctttgag gatgtaactt cagtgaacac
     2941 ctacttcaag aactgcacat ttattgacac tgtttttgac aacacagatt ttgagccata
     3001 taaattcatt gacagtgaat ttaaaaactg ctcgtttttt cacaacaaga cgggatgtca
     3061 gattaccttt gatgatgact atagtgccta ctggatttat tttgtcaact ttctggggac
     3121 attggcagta ttgccaggga acattgtgtc tgctctgctg atggacagaa ttgggcgctt
     3181 aacaatgcta ggtggctcta tggtgctttc ggggatcagc tgtttcttcc tttggttcgg
     3241 caccagtgaa tccatgatga taggcatgct gtgtctgtac aatggattga ccatctcagc
     3301 ctggaactct cttgacgtgg tcactgtgga actgtacccc acagaccgga gggcaacagg
     3361 ctttggcttc ttaaatgcgc tatgcaaggc agcagccgtc ctgggaaact taatatttgg
     3421 ctctctggtc agcatcacca aatcaatccc catcctgctg gcttctactg tgctcgtgtg
     3481 tggaggactc gttgggctgt gcctgcctga cacacgaacc caggttctga tgtgattgcc
     3541 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     3601 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     3661 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3721 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     3781 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     3841 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     3901 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3961 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     4021 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     4081 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     4141 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     4201 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     4261 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     4321 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     4381 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     4441 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     4501 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     4561 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     4621 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     4681 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     4741 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     4801 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     4861 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     4921 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtc
//
