LOCUS       sequence-168853-        8467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to 
            target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, 
            Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y
  TITLE     Marker-free coselection for CRISPR-driven genome editing in human 
            cells.
  JOURNAL   Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265.
  PUBMED    28417998
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265."
COMMENT     Sequence Label: pY036_ATP1A1_G5
FEATURES             Location/Qualifiers
     source          1..8467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        154..402
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     154..174
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     325..344
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        528..831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        833..1110
                     /label=chicken beta-actin promoter
     intron          1111..1339
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1371..1380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1383..1403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1428..5345
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      5065..5074
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5346..5393
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5400..5480
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5508..5525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5514..5721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5730..5870
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5730..5859
                     /label=AAV2 ITR
     rep_origin      5945..6400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6032..6051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6242..6263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6417..6436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6536..6558
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6596..6614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6682..6786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6787..6855,6856..7647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7005..7024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7818..8406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8307..8326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggcctct agagagggcc tatttcccat gattccttca
      181 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
      241 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      301 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      361 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgtaatttc tactcttgta
      421 gattagtaca catcagatat cttttttttc aattgcatga agaatctgct tagggttagg
      481 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gggtacccgt tacataactt
      541 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      601 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      661 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      721 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      781 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
      841 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      901 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
      961 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     1021 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     1081 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt
     1141 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     1201 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta
     1261 agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc
     1321 tgaaatcact ttttttcagg ttggaccggc tagcgtttaa acttaagctt gccaccatgg
     1381 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccaca cagttcgagg
     1441 gctttaccaa cctgtatcag gtgagcaaga cactgcggtt tgagctgatc ccacagggca
     1501 agaccctgaa gcacatccag gagcagggct tcatcgagga ggacaaggcc cgcaatgatc
     1561 actacaagga gctgaagccc atcatcgatc ggatctacaa gacctatgcc gaccagtgcc
     1621 tgcagctggt gcagctggat tgggagaacc tgagcgccgc catcgactcc tatagaaagg
     1681 agaaaaccga ggagacaagg aacgccctga tcgaggagca ggccacatat cgcaatgcca
     1741 tccacgacta cttcatcggc cggacagaca acctgaccga tgccatcaat aagagacacg
     1801 ccgagatcta caagggcctg ttcaaggccg agctgtttaa tggcaaggtg ctgaagcagc
     1861 tgggcaccgt gaccacaacc gagcacgaga acgccctgct gcggagcttc gacaagttta
     1921 caacctactt ctccggcttt tatgagaaca ggaagaacgt gttcagcgcc gaggatatca
     1981 gcacagccat cccacaccgc atcgtgcagg acaacttccc caagtttaag gagaattgtc
     2041 acatcttcac acgcctgatc accgccgtgc ccagcctgcg ggagcacttt gagaacgtga
     2101 agaaggccat cggcatcttc gtgagcacct ccatcgagga ggtgttttcc ttcccttttt
     2161 ataaccagct gctgacacag acccagatcg acctgtataa ccagctgctg ggaggaatct
     2221 ctcgggaggc aggcaccgag aagatcaagg gcctgaacga ggtgctgaat ctggccatcc
     2281 agaagaatga tgagacagcc cacatcatcg cctccctgcc acacagattc atccccctgt
     2341 ttaagcagat cctgtccgat aggaacaccc tgtctttcat cctggaggag tttaagagcg
     2401 acgaggaagt gatccagtcc ttctgcaagt acaagacact gctgagaaac gagaacgtgc
     2461 tggagacagc cgaggccctg tttaacgagc tgaacagcat cgacctgaca cacatcttca
     2521 tcagccacaa gaagctggag acaatcagca gcgccctgtg cgaccactgg gatacactga
     2581 ggaatgccct gtatgagcgg agaatctccg agctgacagg caagatcacc aagtctgcca
     2641 aggagaaggt gcagcgcagc ctgaagcacg aggatatcaa cctgcaggag atcatctctg
     2701 ccgcaggcaa ggagctgagc gaggccttca agcagaaaac cagcgagatc ctgtcccacg
     2761 cacacgccgc cctggatcag ccactgccta caaccctgaa gaagcaggag gagaaggaga
     2821 tcctgaagtc tcagctggac agcctgctgg gcctgtacca cctgctggac tggtttgccg
     2881 tggatgagtc caacgaggtg gaccccgagt tctctgcccg gctgaccggc atcaagctgg
     2941 agatggagcc ttctctgagc ttctacaaca aggccagaaa ttatgccacc aagaagccct
     3001 actccgtgga gaagttcaag ctgaactttc agatgcctac actggcctct ggctgggacg
     3061 tgaataagga gaagaacaat ggcgccatcc tgtttgtgaa gaacggcctg tactatctgg
     3121 gcatcatgcc aaagcagaag ggcaggtata aggccctgag cttcgagccc acagagaaaa
     3181 ccagcgaggg ctttgataag atgtactatg actacttccc tgatgccgcc aagatgatcc
     3241 caaagtgcag cacccagctg aaggccgtga cagcccactt tcagacccac acaaccccca
     3301 tcctgctgtc caacaatttc atcgagcctc tggagatcac aaaggagatc tacgacctga
     3361 acaatcctga gaaggagcca aagaagtttc agacagccta cgccaagaaa accggcgacc
     3421 agaagggcta cagagaggcc ctgtgcaagt ggatcgactt cacaagggat tttctgtcca
     3481 agtataccaa gacaacctct atcgatctgt ctagcctgcg gccatcctct cagtataagg
     3541 acctgggcga gtactatgcc gagctgaatc ccctgctgta ccacatcagc ttccagagaa
     3601 tcgccgagaa ggagatcatg gatgccgtgg agacaggcaa gctgtacctg ttccagatct
     3661 ataacaagga ctttgccaag ggccaccacg gcaagcctaa tctgcacaca ctgtattgga
     3721 ccggcctgtt ttctccagag aacctggcca agacaagcat caagctgaat ggccaggccg
     3781 agctgttcta ccgccctaag tccaggatga agaggatggc acaccggctg ggagagaaga
     3841 tgctgaacaa gaagctgaag gatcagaaaa ccccaatccc cgacaccctg taccaggagc
     3901 tgtacgacta tgtgaatcac agactgtccc acgacctgtc tgatgaggcc agggccctgc
     3961 tgcccaacgt gatcaccaag gaggtgtctc acgagatcat caaggatagg cgctttacca
     4021 gcgacaagtt ctttttccac gtgcctatca cactgaacta tcaggccgcc aattccccat
     4081 ctaagttcaa ccagagggtg aatgcctacc tgaaggagca ccccgagaca cctatcatcg
     4141 gcatcgatcg gggcgagaga aacctgatct atatcacagt gatcgactcc accggcaaga
     4201 tcctggagca gcggagcctg aacaccatcc agcagtttga ttaccagaag aagctggaca
     4261 acagggagaa ggagagggtg gcagcaaggc aggcctggtc tgtggtgggc acaatcaagg
     4321 atctgaagca gggctatctg agccaggtca tccacgagat cgtggacctg atgatccact
     4381 accaggccgt ggtggtgctg gagaacctga atttcggctt taagagcaag aggaccggca
     4441 tcgccgagaa ggccgtgtac cagcagttcg agaagatgct gatcgataag ctgaattgcc
     4501 tggtgctgaa ggactatcca gcagagaaag tgggaggcgt gctgaaccca taccagctga
     4561 cagaccagtt cacctccttt gccaagatgg gcacccagtc tggcttcctg ttttacgtgc
     4621 ctgccccata tacatctaag atcgatcccc tgaccggctt cgtggacccc ttcgtgtgga
     4681 aaaccatcaa gaatcacgag agccgcaagc acttcctgga gggcttcgac tttctgcact
     4741 acgacgtgaa aaccggcgac ttcatcctgc actttaagat gaacagaaat ctgtccttcc
     4801 agaggggcct gcccggcttt atgcctgcat gggatatcgt gttcgagaag aacgagacac
     4861 agtttgacgc caagggcacc cctttcatcg ccggcaagag aatcgtgcca gtgatcgaga
     4921 atcacagatt caccggcaga taccgggacc tgtatcctgc caacgagctg atcgccctgc
     4981 tggaggagaa gggcatcgtg ttcagggatg gctccaacat cctgccaaag ctgctggaga
     5041 atgacgattc tcacgccatc gacaccatgg tggccctgat ccgcagcgtg ctgcagatgc
     5101 ggaactccaa tgccgccaca ggcgaggact atatcaacag ccccgtgcgc gatctgaatg
     5161 gcgtgtgctt cgactcccgg tttcagaacc cagagtggcc catggacgcc gatgccaatg
     5221 gcgcctacca catcgccctg aagggccagc tgctgctgaa tcacctgaag gagagcaagg
     5281 atctgaagct gcagaacggc atctccaatc aggactggct ggcctacatc caggagctgc
     5341 gcaacaaaag gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct
     5401 acccatacga tgttccagat tacgcttatc cctacgacgt gcctgattat gcatacccat
     5461 atgatgtccc cgactatgcc taagaattcc tagagctcgc tgatcagcct cgactgtgcc
     5521 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5581 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5641 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5701 gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc
     5761 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     5821 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     5881 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     5941 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6001 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6061 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6121 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     6181 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6241 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     6301 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6361 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     6421 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     6481 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6541 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     6601 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     6661 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6721 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6781 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6841 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6901 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6961 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7021 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7081 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7141 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7201 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7261 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7321 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7381 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7441 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7501 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7561 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7621 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8461 cacatgt
//
