LOCUS       sequence-168854-        4155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GABRA6 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GABRA6_WT
FEATURES             Location/Qualifiers
     source          1..4155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1555..1654)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1671..1690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1672..1690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1695..1711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1695..1711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2142..2933
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3026..3614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3515..3534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3768..3785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3944..3971)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4063..4149)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcgtcg tctctgccct ggctgtgcat tattctgtgg ctagaaaatg
      241 ccctagggaa actcgaagtt gaaggcaact tctactcaga aaacgtcagt cggatcctgg
      301 acaacttgct tgaaggctat gacaatcggc tgcggccggg atttggaggt gctgtcactg
      361 aagtcaaaac agacatttat gtgaccagtt ttgggcccgt gtcagatgtg gagatggagt
      421 atacgatgga tgtttttttc cgccagacct ggactgatga gaggttgaag tttggggggc
      481 caactgagat tctgagtctg aataatttga tggtcagtaa aatctggacg cctgacacct
      541 ttttcagaaa tggtaaaaag tccattgctc acaacatgac aactcctaat aaactcttca
      601 gaataatgca gaatggaacc attttataca ccatgaggct taccatcaat gctgactgtc
      661 ccatgaggct ggttaacttt cctatggatg ggcatgcttg tccactcaag tttgggagct
      721 atgcttatcc caaaagtgaa atcatatata cgtggaaaaa aggaccactt tactcagtag
      781 aagtcccaga agaatcttca agccttctcc agtatgatct gattggacaa acagtatcta
      841 gtgagacaat taaatctaac acaggtgaat acgttataat gacagtttac ttccacttgc
      901 aaaggaagat gggctacttc atgatacaga tatacactcc ttgcattatg acagtcattc
      961 tttcccaggt gtctttctgg attaataagg agtccgtccc agcaagaact gtttttggga
     1021 tcaccactgt tttaactatg accactttga gcatcagtgc ccggcactct ttgccaaaag
     1081 tgtcatatgc cactgccatg gattggttca tagctgtttg ctttgcattc gtcttctctg
     1141 ctcttatcga gttcgcagct gtcaactact ttaccaatct tcagacacag aaggccaaaa
     1201 ggaaggcaca gtttgcagcc ccacccacag tgacaatatc aaaagctact gaacctttgg
     1261 aagctgagat tgttttgcat cctgactcca aatatcatct gaagaaaagg atcacttctc
     1321 tgtctttgcc aatagtttca tcttccgagg ccaataaagt gctcacgaga gcgcccatct
     1381 tacaatcaac acctgtcaca cccccaccac tctcgccagc ctttggaggc accagtaaaa
     1441 tagaccagta ttctcgaatt ctcttcccag ttgcatttgc aggattcaac cttgtgtact
     1501 gggtagttta tctttccaaa gatacaatgg aagtgagtag cagtgttgaa tgattgccaa
     1561 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1621 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1681 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1741 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     1801 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1861 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1921 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1981 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2041 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2101 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2161 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2221 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2281 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2341 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2401 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2461 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2521 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2581 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2641 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2701 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2761 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2821 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2881 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2941 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3001 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3061 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3901 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3961 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4021 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4081 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4141 ttttatttga tgcct
//
