LOCUS       sequence-168857-        8507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1
            G5 guide in combination with a user-specified guide. Cloning of 
            oligos for the second guide using BbsI sites. PY036-like plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, 
            Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y
  TITLE     Marker-free coselection for CRISPR-driven genome editing in human 
            cells.
  JOURNAL   Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265.
  PUBMED    28417998
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265."
COMMENT     Sequence Label: pY036_ATP1A1_G5_Array
FEATURES             Location/Qualifiers
     source          1..8507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        154..402
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     154..174
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     325..344
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        568..871
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        873..1150
                     /label=chicken beta-actin promoter
     intron          1151..1379
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1411..1420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1423..1443
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1468..5385
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      5105..5114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5386..5433
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5440..5520
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5548..5565)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5554..5761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5770..5910
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5770..5899
                     /label=AAV2 ITR
     rep_origin      5985..6440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6072..6091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6282..6303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6457..6476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6576..6598
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6636..6654)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6722..6826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6827..6895,6896..7687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7045..7064)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7858..8446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8347..8366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggcctct agagagggcc tatttcccat gattccttca
      181 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
      241 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      301 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      361 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgtaatttc tactcttgta
      421 gatgggtctt cgagaagacc taatttctac tcttgtagat tagtacacat cagatatctt
      481 ctcttttttc aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      541 tgtacgggcc agatatacgc gggtacccgt tacataactt acggtaaatg gcccgcctgg
      601 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      661 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      721 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      781 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      841 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
      901 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
      961 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg
     1021 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
     1081 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     1141 gcggcgggcg ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct
     1201 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1261 gcccttctcc tccgggctgt aattagctga gcaagaggta agggtttaag ggatggttgg
     1321 ttggtggggt attaatgttt aattacctgg agcacctgcc tgaaatcact ttttttcagg
     1381 ttggaccggc tagcgtttaa acttaagctt gccaccatgg ccccaaagaa gaagcggaag
     1441 gtcggtatcc acggagtccc agcagccaca cagttcgagg gctttaccaa cctgtatcag
     1501 gtgagcaaga cactgcggtt tgagctgatc ccacagggca agaccctgaa gcacatccag
     1561 gagcagggct tcatcgagga ggacaaggcc cgcaatgatc actacaagga gctgaagccc
     1621 atcatcgatc ggatctacaa gacctatgcc gaccagtgcc tgcagctggt gcagctggat
     1681 tgggagaacc tgagcgccgc catcgactcc tatagaaagg agaaaaccga ggagacaagg
     1741 aacgccctga tcgaggagca ggccacatat cgcaatgcca tccacgacta cttcatcggc
     1801 cggacagaca acctgaccga tgccatcaat aagagacacg ccgagatcta caagggcctg
     1861 ttcaaggccg agctgtttaa tggcaaggtg ctgaagcagc tgggcaccgt gaccacaacc
     1921 gagcacgaga acgccctgct gcggagcttc gacaagttta caacctactt ctccggcttt
     1981 tatgagaaca ggaagaacgt gttcagcgcc gaggatatca gcacagccat cccacaccgc
     2041 atcgtgcagg acaacttccc caagtttaag gagaattgtc acatcttcac acgcctgatc
     2101 accgccgtgc ccagcctgcg ggagcacttt gagaacgtga agaaggccat cggcatcttc
     2161 gtgagcacct ccatcgagga ggtgttttcc ttcccttttt ataaccagct gctgacacag
     2221 acccagatcg acctgtataa ccagctgctg ggaggaatct ctcgggaggc aggcaccgag
     2281 aagatcaagg gcctgaacga ggtgctgaat ctggccatcc agaagaatga tgagacagcc
     2341 cacatcatcg cctccctgcc acacagattc atccccctgt ttaagcagat cctgtccgat
     2401 aggaacaccc tgtctttcat cctggaggag tttaagagcg acgaggaagt gatccagtcc
     2461 ttctgcaagt acaagacact gctgagaaac gagaacgtgc tggagacagc cgaggccctg
     2521 tttaacgagc tgaacagcat cgacctgaca cacatcttca tcagccacaa gaagctggag
     2581 acaatcagca gcgccctgtg cgaccactgg gatacactga ggaatgccct gtatgagcgg
     2641 agaatctccg agctgacagg caagatcacc aagtctgcca aggagaaggt gcagcgcagc
     2701 ctgaagcacg aggatatcaa cctgcaggag atcatctctg ccgcaggcaa ggagctgagc
     2761 gaggccttca agcagaaaac cagcgagatc ctgtcccacg cacacgccgc cctggatcag
     2821 ccactgccta caaccctgaa gaagcaggag gagaaggaga tcctgaagtc tcagctggac
     2881 agcctgctgg gcctgtacca cctgctggac tggtttgccg tggatgagtc caacgaggtg
     2941 gaccccgagt tctctgcccg gctgaccggc atcaagctgg agatggagcc ttctctgagc
     3001 ttctacaaca aggccagaaa ttatgccacc aagaagccct actccgtgga gaagttcaag
     3061 ctgaactttc agatgcctac actggcctct ggctgggacg tgaataagga gaagaacaat
     3121 ggcgccatcc tgtttgtgaa gaacggcctg tactatctgg gcatcatgcc aaagcagaag
     3181 ggcaggtata aggccctgag cttcgagccc acagagaaaa ccagcgaggg ctttgataag
     3241 atgtactatg actacttccc tgatgccgcc aagatgatcc caaagtgcag cacccagctg
     3301 aaggccgtga cagcccactt tcagacccac acaaccccca tcctgctgtc caacaatttc
     3361 atcgagcctc tggagatcac aaaggagatc tacgacctga acaatcctga gaaggagcca
     3421 aagaagtttc agacagccta cgccaagaaa accggcgacc agaagggcta cagagaggcc
     3481 ctgtgcaagt ggatcgactt cacaagggat tttctgtcca agtataccaa gacaacctct
     3541 atcgatctgt ctagcctgcg gccatcctct cagtataagg acctgggcga gtactatgcc
     3601 gagctgaatc ccctgctgta ccacatcagc ttccagagaa tcgccgagaa ggagatcatg
     3661 gatgccgtgg agacaggcaa gctgtacctg ttccagatct ataacaagga ctttgccaag
     3721 ggccaccacg gcaagcctaa tctgcacaca ctgtattgga ccggcctgtt ttctccagag
     3781 aacctggcca agacaagcat caagctgaat ggccaggccg agctgttcta ccgccctaag
     3841 tccaggatga agaggatggc acaccggctg ggagagaaga tgctgaacaa gaagctgaag
     3901 gatcagaaaa ccccaatccc cgacaccctg taccaggagc tgtacgacta tgtgaatcac
     3961 agactgtccc acgacctgtc tgatgaggcc agggccctgc tgcccaacgt gatcaccaag
     4021 gaggtgtctc acgagatcat caaggatagg cgctttacca gcgacaagtt ctttttccac
     4081 gtgcctatca cactgaacta tcaggccgcc aattccccat ctaagttcaa ccagagggtg
     4141 aatgcctacc tgaaggagca ccccgagaca cctatcatcg gcatcgatcg gggcgagaga
     4201 aacctgatct atatcacagt gatcgactcc accggcaaga tcctggagca gcggagcctg
     4261 aacaccatcc agcagtttga ttaccagaag aagctggaca acagggagaa ggagagggtg
     4321 gcagcaaggc aggcctggtc tgtggtgggc acaatcaagg atctgaagca gggctatctg
     4381 agccaggtca tccacgagat cgtggacctg atgatccact accaggccgt ggtggtgctg
     4441 gagaacctga atttcggctt taagagcaag aggaccggca tcgccgagaa ggccgtgtac
     4501 cagcagttcg agaagatgct gatcgataag ctgaattgcc tggtgctgaa ggactatcca
     4561 gcagagaaag tgggaggcgt gctgaaccca taccagctga cagaccagtt cacctccttt
     4621 gccaagatgg gcacccagtc tggcttcctg ttttacgtgc ctgccccata tacatctaag
     4681 atcgatcccc tgaccggctt cgtggacccc ttcgtgtgga aaaccatcaa gaatcacgag
     4741 agccgcaagc acttcctgga gggcttcgac tttctgcact acgacgtgaa aaccggcgac
     4801 ttcatcctgc actttaagat gaacagaaat ctgtccttcc agaggggcct gcccggcttt
     4861 atgcctgcat gggatatcgt gttcgagaag aacgagacac agtttgacgc caagggcacc
     4921 cctttcatcg ccggcaagag aatcgtgcca gtgatcgaga atcacagatt caccggcaga
     4981 taccgggacc tgtatcctgc caacgagctg atcgccctgc tggaggagaa gggcatcgtg
     5041 ttcagggatg gctccaacat cctgccaaag ctgctggaga atgacgattc tcacgccatc
     5101 gacaccatgg tggccctgat ccgcagcgtg ctgcagatgc ggaactccaa tgccgccaca
     5161 ggcgaggact atatcaacag ccccgtgcgc gatctgaatg gcgtgtgctt cgactcccgg
     5221 tttcagaacc cagagtggcc catggacgcc gatgccaatg gcgcctacca catcgccctg
     5281 aagggccagc tgctgctgaa tcacctgaag gagagcaagg atctgaagct gcagaacggc
     5341 atctccaatc aggactggct ggcctacatc caggagctgc gcaacaaaag gccggcggcc
     5401 acgaaaaagg ccggccaggc aaaaaagaaa aagggatcct acccatacga tgttccagat
     5461 tacgcttatc cctacgacgt gcctgattat gcatacccat atgatgtccc cgactatgcc
     5521 taagaattcc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5581 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5641 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5701 gtggggtggg gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg
     5761 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     5821 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     5881 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     5941 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     6001 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6061 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6121 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6181 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6241 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6301 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     6361 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6421 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     6481 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6541 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6601 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     6661 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6721 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6781 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6841 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6901 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6961 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7021 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7081 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7141 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7201 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7261 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7321 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7381 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7441 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7501 tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc
     7561 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7621 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7681 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7741 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7801 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7861 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7921 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7981 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8041 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8101 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8161 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8221 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8281 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8341 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8401 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8461 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
