LOCUS       sequence-168863-        6192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing JAK2 , part of the Target 
            Accelerator Plasmid Collection. *Please note this is not a true WT 
            JAK2 sequence and contains a V617F mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_JAK2_WT
FEATURES             Location/Qualifiers
     source          1..6192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4071..4170)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4187..4206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4188..4206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4211..4227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4211..4227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4658..5449
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5542..6130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6031..6050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgggaatgg cctgccttac gatgacagaa atggagggaa catccacctc
      721 ttctatatat cagaatggtg atatttctgg aaatgccaat tctatgaagc aaatagatcc
      781 agttcttcag gtgtatcttt accattccct tgggaaatct gaggcagatt atctgacctt
      841 tccatctggg gagtatgttg cagaagaaat ctgtattgct gcttctaaag cttgtggtat
      901 cacacctgtg tatcataata tgtttgcttt aatgagtgaa acagaaagga tctggtatcc
      961 acccaaccat gtcttccata tagatgagtc aaccaggcat aatgtactct acagaataag
     1021 attttacttt cctcgttggt attgcagtgg cagcaacaga gcctatcggc atggaatatc
     1081 tcgaggtgct gaagctcctc ttcttgatga ctttgtcatg tcttacctct ttgctcagtg
     1141 gcggcatgat tttgtgcacg gatggataaa agtacctgtg actcatgaaa cacaggaaga
     1201 atgtcttggg atggcagtgt tagatatgat gagaatagcc aaagaaaacg atcaaacccc
     1261 actggccatc tataactcta tcagctacaa gacattctta ccaaaatgta ttcgagcaaa
     1321 gatccaagac tatcatattt tgacaaggaa gcgaataagg tacagatttc gcagatttat
     1381 tcagcaattc agccaatgca aagccactgc cagaaacttg aaacttaagt atcttataaa
     1441 tctggaaact ctgcagtctg ccttctacac agagaaattt gaagtaaaag aacctggaag
     1501 tggtccttca ggtgaggaga tttttgcaac cattataata actggaaacg gtggaattca
     1561 gtggtcaaga gggaaacata aagaaagtga gacactgaca gaacaggatt tacagttata
     1621 ttgcgatttt cctaatatta ttgatgtcag tattaagcaa gcaaaccaag agggttcaaa
     1681 tgaaagccga gttgtaacta tccataagca agatggtaaa aatctggaaa ttgaacttag
     1741 ctcattaagg gaagctttgt ctttcgtgtc attaattgat ggatattata gattaactgc
     1801 agatgcacat cattacctct gtaaagaagt agcacctcca gccgtgcttg aaaatataca
     1861 aagcaactgt catggcccaa tttcgatgga ttttgccatt agtaaactga agaaagcagg
     1921 taatcagact ggactgtatg tacttcgatg cagtcctaag gactttaata aatatttttt
     1981 gacttttgct gtcgagcgag aaaatgtcat tgaatataaa cactgtttga ttacaaaaaa
     2041 tgagaatgaa gagtacaacc tcagtgggac aaagaagaac ttcagcagtc ttaaagatct
     2101 tttgaattgt taccagatgg aaactgttcg ctcagacaat ataattttcc agtttactaa
     2161 atgctgtccc ccaaagccaa aagataaatc aaaccttcta gtcttcagaa cgaatggtgt
     2221 ttctgatgta ccaacctcac caacattaca gaggcctact catatgaacc aaatggtgtt
     2281 tcacaaaatc agaaatgaag atttgatatt taatgaaagc cttggccaag gcacttttac
     2341 aaagattttt aaaggcgtac gaagagaagt aggagactac ggtcaactgc atgaaacaga
     2401 agttctttta aaagttctgg ataaagcaca cagaaactat tcagagtctt tctttgaagc
     2461 agcaagtatg atgagcaagc tttctcacaa gcatttggtt ttaaattatg gagtatgttt
     2521 ctgtggagac gagaatattc tggttcagga gtttgtaaaa tttggatcac tagatacata
     2581 tctgaaaaag aataaaaatt gtataaatat attatggaaa cttgaagttg ctaaacagtt
     2641 ggcatgggcc atgcattttc tagaagaaaa cacccttatt catgggaatg tatgtgccaa
     2701 aaatattctg cttatcagag aagaagacag gaagacagga aatcctcctt tcatcaaact
     2761 tagtgatcct ggcattagta ttacagtttt gccaaaggac attcttcagg agagaatacc
     2821 atgggtacca cctgaatgca ttgaaaatcc taaaaattta aatttggcaa cagacaaatg
     2881 gagttttggt accactttgt gggaaatctg cagtggagga gataaacctc taagtgctct
     2941 ggattctcaa agaaagctac aattttatga agataggcat cagcttcctg caccaaagtg
     3001 ggcagaatta gcaaacctta taaataattg tatggattat gaaccagatt tcaggccttc
     3061 tttcagagcc atcatacgag atcttaacag tttgtttact ccagattatg aactattaac
     3121 agaaaatgac atgttaccaa atatgaggat aggtgcccta gggttttctg gtgcctttga
     3181 agaccgggat cctacacagt ttgaagagag acatttgaaa tttctacagc aacttggcaa
     3241 gggtaatttt gggagtgtgg agatgtgccg gtatgaccct ctacaggaca acactgggga
     3301 ggtggtcgct gtaaaaaagc ttcagcatag tactgaagag cacctaagag actttgaaag
     3361 ggaaattgaa atcctgaaat ccctacagca tgacaacatt gtaaagtaca agggagtgtg
     3421 ctacagtgct ggtcggcgta atctaaaatt aattatggaa tatttaccat atggaagttt
     3481 acgagactat cttcaaaaac ataaagaacg gatagatcac ataaaacttc tgcagtacac
     3541 atctcagata tgcaagggta tggagtatct tggtacaaaa aggtatatcc acagggatct
     3601 ggcaacgaga aatatattgg tggagaacga gaacagagtt aaaattggag attttgggtt
     3661 aaccaaagtc ttgccacaag acaaagaata ctataaagta aaagaacctg gtgaaagtcc
     3721 catattctgg tatgctccag aatcactgac agagagcaag ttttctgtgg cctcagatgt
     3781 ttggagcttt ggagtggttc tgtatgaact tttcacatac attgagaaga gtaaaagtcc
     3841 accagcggaa tttatgcgta tgattggcaa tgacaaacaa ggacagatga tcgtgttcca
     3901 tttgatagaa cttttgaaga ataatggaag attaccaaga ccagatggat gcccagatga
     3961 gatctatatg atcatgacag aatgctggaa caataatgta aatcaacgcc cctcctttag
     4021 ggatctagct cttcgagtgg atcaaataag ggataacatg gctggatgat tgccaacttt
     4081 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     4141 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     4201 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     4261 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     4321 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     4381 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     4441 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     4501 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     4561 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     4621 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     4681 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     4741 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     4801 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     4861 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     4921 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     4981 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     5041 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     5101 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     5161 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     5221 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     5281 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     5341 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     5401 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     5461 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     5521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6181 tgctcacatg tt
//
