LOCUS       sequence-168868-        4620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAM116A ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FAM116A_WT
FEATURES             Location/Qualifiers
     source          1..4620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2020..2119)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2136..2155)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2137..2155)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2160..2176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2160..2176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2607..3398
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3491..4079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3980..3999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4233..4250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4409..4436)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4528..4614)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggctttg aggggccctg cgggcttggg gcccggctct cgaaggccgt
      241 tggacgaagc ggtggcaggg gccgagggcc gcgaggcgcc ggcccttgtg gcggcgggag
      301 gcgcgccaga ggacgatgaa gaggacgatg gccgtggccg gggcctgctg cgctgggaca
      361 gcttctccgc ctggctgcac tgcgtgtgtg tggtgggctt cgacctggag ctgggccagg
      421 ccgtggaggt aatttatcct cagcattcca aacttactga cagagaaaaa accaatattt
      481 gctatttgtc ttttccagat tcaaattcag gttgtcttgg agatacccag ttttgtttta
      541 gatttcgaca gtcttctggg aggagggtgt cgctgcattg tctcctggat caatttgaca
      601 aagatttacc agtttactta aagaaggatc ctgcttattt ttatggatat gtgtatttcc
      661 gacaagttcg agataaaact ctaaaaagag gctactttca gaagtccttg gttttgatca
      721 gcaaactacc ttatattcat ttttttcaca ctgtgctcaa acagatagca ccagagtatt
      781 ttgaaaagaa tgaaccttat ttggaagcag cttgtaatga tgttgatcga tggcctgccc
      841 cagtgccagg gaaaacatta cacctgccaa tcatgggggt ggtaatgaag gtacggattc
      901 ccacatgtca tgacaagcct gggacaactc aaatagtgca gttaactcag caggtggaca
      961 caaatatatc tgttatttta cctactgttc atgaggtgga tattttcagg tgtttctgcc
     1021 cagttttcct tcatagtcag atgctctggg agctggtgct gttgggggag ccccttgtgg
     1081 ttatggcgcc atcaccatcg gaatcatcag agactgtatt ggcacttgtt aactgtattt
     1141 ctccattaaa gtacttcagt gatttccgac cttatttcac tattcatgat agtgaattca
     1201 aagaatatac tacccgtacg caagctccgc cctcagttat attaggagta accaaccctt
     1261 tttttgctaa gacactccag cactggccac acattattcg aataggagac cttaaaccta
     1321 caggtgaaat tcctaagcag gttaaagtga aaaaactgaa gaatctaaag actctggatt
     1381 ccaaacctgg agtttatact tcatataagc catatttaaa tagagatgaa gagatcataa
     1441 aacaattaca gaagggtgta caacagaaac gtccttctga ggctcaaagt gttattcttc
     1501 gacgctattt tttggaactg acacaaagtt tcatcattcc attagaaaga tatgtggcaa
     1561 gcttgatgcc tttgcagaaa agtatttccc catggaagag tccacctcaa ttaagacagt
     1621 ttcttccaga agaatttatg aaaacacttg agaaaacagg acctcagcta acctctagaa
     1681 taaaaggcga ttggattgga ctttaccggc atttcctaaa gtctccaaat tttgatggct
     1741 ggtttaagac ccggaggaag gaaatgaccc aaaaattgga ggcactccat ctagaagctc
     1801 tttgtgaaga ggacttactt ctctggatcc agaaacacac agaagtagaa acagtagacc
     1861 ttgtcttgaa gctgaaaaat aagctgttgc aggctgatcg agagcactta cctgtgaaac
     1921 ctgacactat ggaaaagtta cggacacaca tagatgccat tatcttagca ttgccagagg
     1981 acttgcaagg catactgctc aaaacgggca tgacatgatt gccaactttc ttgtacaaag
     2041 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2101 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2161 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2221 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     2281 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2341 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2401 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2461 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2521 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2581 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2641 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2701 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2761 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2821 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2881 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2941 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     3001 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     3061 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     3121 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     3181 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3241 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3301 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3361 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3421 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3481 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3541 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3601 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3661 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3721 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3781 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3841 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3901 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3961 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4021 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4081 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4141 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4201 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4261 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4321 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4381 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4441 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4501 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4561 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
//
